Experiment set16IT047 for Bacteroides thetaiotaomicron VPI-5482

D-Trehalose dihydrate 20mM carbon source

Group: carbon source
Media: Varel_Bryant_medium + D-Trehalose dihydrate (20 mM)
Culturing: Btheta_ML6a, 24-well transparent microplate, Anaerobic, at 37 (C), shaken=0 rpm
By: Valentine on 6/30/20
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 2 genes in this experiment

For carbon source D-Trehalose dihydrate in Bacteroides thetaiotaomicron VPI-5482

For carbon source D-Trehalose dihydrate across organisms

SEED Subsystems

Subsystem	#Specific
Inositol catabolism	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Streptomycin biosynthesis
• Inositol phosphate metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
myo-inositol degradation II	5	1	1
myo-inositol degradation I	7	1	1
myo-, chiro- and scyllo-inositol degradation	10	1	1
streptomycin biosynthesis	18	3	1