Experiment set16IT042 for Bacteroides thetaiotaomicron VPI-5482
Agro_defined_trehalose 1x carbon source
Group: carbon sourceMedia: Varel_Bryant_medium + 1x Agro_defined_trehalose
Culturing: Btheta_ML6a, 24-well transparent microplate, Anaerobic, at 37 (C), shaken=0 rpm
By: Valentine on 6/30/20
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)
Agro_defined_trehalose 1x includes: 0.9 g/L Urea, 0.15 g/L Magnesium Sulfate Heptahydrate, 0.025 g/L Calcium chloride dihydrate, 0.01 g/L Iron (II) sulfate heptahydrate, 0.16 g/L Citric Acid, 5.4 g/L Potassium phosphate monobasic, 10.8 g/L Sodium phosphate dibasic heptahydrate, 20 g/L D-Trehalose dihydrate
Specific Phenotypes
For 2 genes in this experiment
For carbon source Agro_defined_trehalose in Bacteroides thetaiotaomicron VPI-5482
For carbon source Agro_defined_trehalose across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Inositol catabolism | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| myo-inositol degradation II | 5 | 1 | 1 |
| myo-inositol degradation I | 7 | 1 | 1 |
| myo-, chiro- and scyllo-inositol degradation | 10 | 1 | 1 |
| streptomycin biosynthesis | 18 | 3 | 1 |