Experiment set16IT038 for Pseudomonas fluorescens FW300-N2E2

Compare to:

Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate C

200 most important genes:

  gene name fitness t score description  
Pf6N2E2_3842 -5.8 -4.0 Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19) compare
Pf6N2E2_71 -5.8 -4.0 Phosphoribosylanthranilate isomerase (EC 5.3.1.24) compare
Pf6N2E2_3751 -5.1 -11.4 Glutamate synthase [NADPH] small chain (EC 1.4.1.13) compare
Pf6N2E2_2323 -5.1 -3.5 Fumarate and nitrate reduction regulatory protein compare
Pf6N2E2_3782 -5.1 -4.9 Phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19) compare
Pf6N2E2_3253 -5.0 -4.9 ATP phosphoribosyltransferase (EC 2.4.2.17) (from data) compare
Pf6N2E2_4826 -4.9 -8.1 Indole-3-glycerol phosphate synthase (EC 4.1.1.48) compare
Pf6N2E2_4473 -4.9 -11.8 HlyD family secretion protein compare
Pf6N2E2_4597 -4.9 -6.7 Homoserine O-acetyltransferase (EC 2.3.1.31) compare
Pf6N2E2_64 -4.9 -5.5 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33) compare
Pf6N2E2_4564 -4.8 -9.2 Dihydroxy-acid dehydratase (EC 4.2.1.9) compare
Pf6N2E2_4472 -4.8 -16.8 ABC-type multidrug transport system, permease component compare
Pf6N2E2_736 -4.8 -4.6 hypothetical protein compare
Pf6N2E2_3645 -4.7 -13.2 Type I secretion outer membrane protein, TolC precursor compare
Pf6N2E2_3839 -4.6 -5.4 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) (from data) compare
Pf6N2E2_77 -4.6 -3.1 O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48) compare
Pf6N2E2_4825 -4.5 -9.7 Anthranilate phosphoribosyltransferase (EC 2.4.2.18) compare
Pf6N2E2_5239 -4.5 -14.5 Putative sulfate permease conserved
Pf6N2E2_3610 -4.5 -4.3 ATP phosphoribosyltransferase regulatory subunit (EC 2.4.2.17) compare
Pf6N2E2_4596 -4.4 -9.8 Homoserine O-acetyltransferase (EC 2.3.1.31) compare
Pf6N2E2_63 -4.3 -4.9 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33) compare
Pf6N2E2_5177 -4.3 -11.6 Ketol-acid reductoisomerase (EC 1.1.1.86) compare
Pf6N2E2_3752 -4.1 -21.2 Glutamate synthase [NADPH] large chain (EC 1.4.1.13) compare
Pf6N2E2_5392 -4.1 -2.8 Molybdenum cofactor biosynthesis protein MoaC compare
Pf6N2E2_2277 -4.1 -2.8 NADPH-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.13) compare
Pf6N2E2_66 -4.1 -10.2 3-isopropylmalate dehydrogenase (EC 1.1.1.85) compare
Pf6N2E2_1294 -4.0 -3.8 Carbon starvation protein A compare
Pf6N2E2_735 -4.0 -14.1 Ribonucleotide reductase of class III (anaerobic), large subunit (EC 1.17.4.2) conserved
Pf6N2E2_5728 -3.9 -6.0 Phosphoribosylglycinamide formyltransferase (EC 2.1.2.2) compare
Pf6N2E2_5223 -3.8 -6.3 Molybdopterin biosynthesis protein MoeB compare
Pf6N2E2_477 -3.8 -7.9 Branched-chain amino acid aminotransferase (EC 2.6.1.42) compare
Pf6N2E2_2218 -3.7 -2.6 ATP-dependent Clp protease proteolytic subunit (EC 3.4.21.92) compare
Pf6N2E2_1296 -3.7 -7.1 DNA-binding response regulator, LuxR family compare
Pf6N2E2_5258 -3.7 -9.7 Sulfate adenylyltransferase subunit 1 (EC 2.7.7.4) / Adenylylsulfate kinase (EC 2.7.1.25) compare
Pf6N2E2_3783 -3.7 -6.5 phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31) (from data) compare
Pf6N2E2_5394 -3.6 -4.2 Molybdenum cofactor biosynthesis protein MoaE compare
Pf6N2E2_3841 -3.5 -4.8 Imidazole glycerol phosphate synthase amidotransferase subunit (EC 2.4.2.-) compare
Pf6N2E2_4047 -3.5 -4.8 Tryptophan synthase alpha chain (EC 4.2.1.20) compare
Pf6N2E2_732 -3.4 -8.2 Respiratory nitrate reductase gamma chain (EC 1.7.99.4) compare
Pf6N2E2_3630 -3.4 -6.8 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf6N2E2_3252 -3.4 -9.3 Histidinol dehydrogenase (EC 1.1.1.23) compare
Pf6N2E2_4797 -3.4 -2.2 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase (EC 2.7.6.3) compare
Pf6N2E2_3932 -3.4 -4.5 D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95) compare
Pf6N2E2_729 -3.3 -16.8 Respiratory nitrate reductase alpha chain (EC 1.7.99.4) conserved
Pf6N2E2_5242 -3.3 -2.2 Malate:quinone oxidoreductase (EC 1.1.5.4) compare
Pf6N2E2_1032 -3.3 -1.6 Alkanesulfonates ABC transporter ATP-binding protein / Sulfonate ABC transporter, ATP-binding subunit SsuB compare
Pf6N2E2_737 -3.2 -2.2 Ribonucleotide reductase of class III (anaerobic), activating protein (EC 1.97.1.4) compare
Pf6N2E2_725 -3.2 -6.7 Nitrate/nitrite response regulator protein compare
Pf6N2E2_4351 -3.2 -12.9 Predicted signal transduction protein compare
Pf6N2E2_5666 -3.0 -12.7 Arginine N-succinyltransferase, alpha subunit (EC 2.3.1.109) compare
Pf6N2E2_4048 -3.0 -6.1 Tryptophan synthase beta chain (EC 4.2.1.20) compare
Pf6N2E2_2217 -2.9 -3.9 ATP-dependent Clp protease ATP-binding subunit ClpX compare
Pf6N2E2_4471 -2.9 -7.6 ABC-type multidrug transport system, permease component compare
Pf6N2E2_5175 -2.9 -5.4 Acetolactate synthase large subunit (EC 2.2.1.6) compare
Pf6N2E2_5176 -2.9 -3.4 Acetolactate synthase small subunit (EC 2.2.1.6) compare
Pf6N2E2_5894 -2.7 -8.2 Putative threonine efflux protein compare
Pf6N2E2_4181 -2.7 -1.3 Organic hydroperoxide resistance protein compare
Pf6N2E2_3074 -2.6 -1.4 FIG00960973: hypothetical protein compare
Pf6N2E2_4372 -2.6 -3.9 FIG00460773: hypothetical protein compare
Pf6N2E2_3300 -2.5 -2.3 Predicted D-glucarate or D-galactorate regulator, GntR family compare
Pf6N2E2_5257 -2.5 -7.2 Sulfate adenylyltransferase subunit 2 (EC 2.7.7.4) compare
Pf6N2E2_292 -2.5 -9.2 ATP-dependent Clp protease ATP-binding subunit ClpA compare
Pf6N2E2_731 -2.5 -6.2 Respiratory nitrate reductase delta chain (EC 1.7.99.4) compare
Pf6N2E2_4638 -2.4 -1.7 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20) compare
Pf6N2E2_730 -2.4 -10.2 Respiratory nitrate reductase beta chain (EC 1.7.99.4) compare
Pf6N2E2_3170 -2.4 -6.7 2-isopropylmalate synthase (EC 2.3.3.13) compare
Pf6N2E2_728 -2.3 -4.5 Nitrate/nitrite transporter compare
Pf6N2E2_5899 -2.3 -8.3 Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system compare
Pf6N2E2_718 -2.2 -4.7 Pyridoxamine 5'-phosphate oxidase (EC 1.4.3.5) compare
Pf6N2E2_3264 -2.2 -6.5 Uncharacterized protein YrbK clustered with lipopolysaccharide transporters compare
Pf6N2E2_3255 -2.2 -4.4 YrbA protein compare
Pf6N2E2_2519 -2.1 -1.5 Biosynthetic Aromatic amino acid aminotransferase beta (EC 2.6.1.57) compare
Pf6N2E2_2507 -2.1 -7.2 Undecaprenyl-phosphate N-acetylglucosaminyl 1-phosphate transferase (EC 2.7.8.-) compare
Pf6N2E2_432 -2.1 -7.1 Molybdenum ABC transporter, periplasmic molybdenum-binding protein ModA (TC 3.A.1.8.1) compare
Pf6N2E2_431 -2.1 -7.0 Molybdenum transport system permease protein ModB (TC 3.A.1.8.1) compare
Pf6N2E2_5111 -2.1 -3.2 FKBP-type peptidyl-prolyl cis-trans isomerase SlpA (EC 5.2.1.8) compare
Pf6N2E2_2424 -2.1 -2.6 Molybdopterin-guanine dinucleotide biosynthesis protein MobA compare
Pf6N2E2_4187 -2.1 -7.7 Lipid A biosynthesis lauroyl acyltransferase (EC 2.3.1.-) compare
Pf6N2E2_2189 -2.1 -3.9 Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4) compare
Pf6N2E2_3487 -2.0 -7.1 Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4) compare
Pf6N2E2_2073 -2.0 -6.0 Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2) compare
Pf6N2E2_5080 -2.0 -7.8 Putative metal chaperone, involved in Zn homeostasis, GTPase of COG0523 family compare
Pf6N2E2_4813 -2.0 -12.8 Probable two-component sensor, near polyamine transporter compare
Pf6N2E2_2465 -2.0 -5.5 Cys regulon transcriptional activator CysB compare
Pf6N2E2_3658 -2.0 -4.6 Lipid A core - O-antigen ligase and related enzymes compare
Pf6N2E2_2422 -1.9 -8.3 Molybdopterin biosynthesis protein MoeA compare
Pf6N2E2_3659 -1.9 -2.5 Protein fixF compare
Pf6N2E2_1072 -1.8 -5.8 LmbE-like protein compare
Pf6N2E2_1211 -1.8 -2.7 Lactoylglutathione lyase compare
Pf6N2E2_5520 -1.8 -5.9 Sensory box histidine kinase compare
Pf6N2E2_2389 -1.8 -8.3 Phosphoenolpyruvate synthase (EC 2.7.9.2) compare
Pf6N2E2_1295 -1.8 -5.3 Sensor histidine kinase compare
Pf6N2E2_4762 -1.8 -1.6 Biotin synthesis protein BioH compare
Pf6N2E2_3984 -1.7 -10.9 T1SS associated transglutaminase-like cysteine proteinase LapP compare
Pf6N2E2_722 -1.7 -5.6 FIG139928: Putative protease compare
Pf6N2E2_2574 -1.6 -1.5 Flagellar synthesis regulator FleN compare
Pf6N2E2_2506 -1.6 -8.8 nucleotide sugar epimerase/dehydratase WbpM compare
Pf6N2E2_2508 -1.6 -3.4 hypothetical protein compare
Pf6N2E2_4803 -1.6 -1.1 Bis(5'-nucleosyl)-tetraphosphatase, symmetrical (EC 3.6.1.41) compare
Pf6N2E2_2681 -1.6 -1.1 3-phosphoglycerate kinase compare
Pf6N2E2_3661 -1.6 -2.3 hypothetical protein compare
Pf6N2E2_3807 -1.6 -4.9 Histidine utilization repressor compare
Pf6N2E2_4454 -1.6 -5.4 Exopolyphosphatase (EC 3.6.1.11) compare
Pf6N2E2_4743 -1.6 -1.7 hypothetical protein compare
Pf6N2E2_1293 -1.6 -3.0 COG2879, Hypothetical small protein yjiX compare
Pf6N2E2_3940 -1.6 -8.4 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf6N2E2_726 -1.6 -3.4 Nitrate/nitrite sensor protein (EC 2.7.3.-) compare
Pf6N2E2_5339 -1.6 -10.4 ubiquinol cytochrome C oxidoreductase, cytochrome C1 subunit compare
Pf6N2E2_721 -1.6 -6.1 FIG139552: Putative protease compare
Pf6N2E2_723 -1.5 -2.3 FIG138517: Putative lipid carrier protein compare
Pf6N2E2_1932 -1.5 -2.3 Transcriptional regulator, IclR family compare
Pf6N2E2_2822 -1.5 -7.2 Transcription repressor of multidrug efflux pump acrAB operon, TetR (AcrR) family compare
Pf6N2E2_2513 -1.5 -1.5 UDP-glucose dehydrogenase (EC 1.1.1.22) compare
Pf6N2E2_5338 -1.5 -11.3 Ubiquinol--cytochrome c reductase, cytochrome B subunit (EC 1.10.2.2) compare
Pf6N2E2_2510 -1.5 -2.0 Lipid carrier : UDP-N-acetylgalactosaminyltransferase (EC 2.4.1.-) / Alpha-1,3-N-acetylgalactosamine transferase PglA (EC 2.4.1.-); Putative glycosyltransferase compare
Pf6N2E2_3060 -1.5 -3.4 Outer membrane protein H precursor compare
Pf6N2E2_760 -1.5 -9.7 Na+/H+ antiporter NhaB compare
Pf6N2E2_4921 -1.5 -3.7 3,4-dihydroxy-2-butanone 4-phosphate synthase (EC 4.1.99.12) / GTP cyclohydrolase II (EC 3.5.4.25) compare
Pf6N2E2_1411 -1.4 -5.6 Coproporphyrinogen III oxidase, oxygen-independent (EC 1.3.99.22) compare
Pf6N2E2_4273 -1.4 -5.8 ATP-dependent DNA helicase UvrD/PcrA compare
Pf6N2E2_291 -1.4 -3.7 ATP-dependent Clp protease adaptor protein ClpS compare
Pf6N2E2_5299 -1.4 -6.2 Benzoate transport protein compare
Pf6N2E2_204 -1.4 -1.7 hypothetical protein compare
Pf6N2E2_3786 -1.4 -6.2 Twin-arginine translocation protein TatC compare
Pf6N2E2_2572 -1.4 -3.4 Chemotaxis regulator - transmits chemoreceptor signals to flagelllar motor components CheY compare
Pf6N2E2_5328 -1.3 -4.8 FIG00958649: hypothetical protein compare
Pf6N2E2_658 -1.3 -7.6 sensor histidine kinase compare
Pf6N2E2_5665 -1.3 -6.2 Succinylornithine transaminase (EC 2.6.1.81) compare
Pf6N2E2_4818 -1.3 -5.1 Spermidine Putrescine ABC transporter permease component potC (TC_3.A.1.11.1) compare
Pf6N2E2_1617 -1.3 -1.2 COG2199: FOG: GGDEF domain compare
Pf6N2E2_3521 -1.3 -7.4 Sensor histidine kinase/response regulator compare
Pf6N2E2_3251 -1.3 -7.2 histidinol-phosphate aminotransferase (EC 2.6.1.9) (from data) compare
Pf6N2E2_2340 -1.3 -2.9 Transcriptional regulator, GntR family compare
Pf6N2E2_4817 -1.3 -9.9 Spermidine Putrescine ABC transporter permease component PotB (TC 3.A.1.11.1) compare
Pf6N2E2_2825 -1.2 -4.2 RND efflux system, outer membrane lipoprotein CmeC compare
Pf6N2E2_2674 -1.2 -1.0 FIG00953856: hypothetical protein compare
Pf6N2E2_4607 -1.2 -3.3 Uracil phosphoribosyltransferase (EC 2.4.2.9) / Pyrimidine operon regulatory protein PyrR compare
Pf6N2E2_3346 -1.2 -4.5 Quinolinate phosphoribosyltransferase [decarboxylating] (EC 2.4.2.19) compare
Pf6N2E2_1162 -1.2 -1.6 Transmembrane amino acid efflux protein compare
Pf6N2E2_2384 -1.2 -0.8 CmpX compare
Pf6N2E2_3039 -1.2 -6.1 Protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77) compare
Pf6N2E2_727 -1.2 -4.8 Nitrate/nitrite transporter compare
Pf6N2E2_5519 -1.2 -4.0 ATP:Cob(I)alamin adenosyltransferase (EC 2.5.1.17) compare
Pf6N2E2_5157 -1.2 -6.3 Two-component sensor CbrA: intrcellular carbon:nitrogen balance compare
Pf6N2E2_4612 -1.2 -2.4 Glutathione synthetase (EC 6.3.2.3) compare
Pf6N2E2_4097 -1.2 -8.2 FIG00953365: hypothetical protein compare
Pf6N2E2_289 -1.2 -7.8 Isocitrate dehydrogenase [NADP] (EC 1.1.1.42) compare
Pf6N2E2_2201 -1.2 -3.3 Transmembrane regulator protein PrtR compare
Pf6N2E2_4204 -1.2 -1.1 rRNA small subunit 7-methylguanosine (m7G) methyltransferase GidB compare
Pf6N2E2_2088 -1.2 -1.5 SOS-response repressor and protease LexA (EC 3.4.21.88) compare
Pf6N2E2_3248 -1.1 -1.6 Probable transmembrane protein compare
Pf6N2E2_156 -1.1 -1.7 Carbon storage regulator compare
Pf6N2E2_3611 -1.1 -1.4 HflC protein compare
Pf6N2E2_5593 -1.1 -3.2 Leucyl-tRNA synthetase compare
Pf6N2E2_2752 -1.1 -1.8 Exported zinc metalloprotease YfgC precursor compare
Pf6N2E2_5553 -1.1 -5.9 Quinolinate synthetase (EC 2.5.1.72) compare
Pf6N2E2_5014 -1.1 -0.5 Apolipoprotein N-acyltransferase (EC 2.3.1.-) / Copper homeostasis protein CutE compare
Pf6N2E2_4773 -1.1 -1.6 Coenzyme PQQ synthesis protein D compare
Pf6N2E2_2074 -1.1 -1.5 required for sulfate utilization, putative electron transport protein for sulfite reductase (from data) compare
Pf6N2E2_2180 -1.1 -4.0 Arginine N-succinyltransferase, alpha subunit (EC 2.3.1.109) compare
Pf6N2E2_938 -1.1 -5.2 hypothetical protein compare
Pf6N2E2_4049 -1.1 -6.7 Glycine cleavage system transcriptional activator compare
Pf6N2E2_5679 -1.1 -3.9 hypothetical protein compare
Pf6N2E2_1136 -1.1 -1.3 Transcriptional regulator, TetR family compare
Pf6N2E2_4414 -1.1 -1.1 FIG00953808: hypothetical protein compare
Pf6N2E2_649 -1.1 -2.2 General secretion pathway protein L compare
Pf6N2E2_2181 -1.1 -1.4 Arginine N-succinyltransferase (EC 2.3.1.109) compare
Pf6N2E2_1876 -1.1 -1.2 O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) compare
Pf6N2E2_1412 -1.1 -2.2 hypothetical protein compare
Pf6N2E2_510 -1.1 -6.7 Predicted transcriptional regulator of the myo-inositol catabolic operon compare
Pf6N2E2_704 -1.0 -2.9 Cytochrome c551/c552 compare
Pf6N2E2_3282 -1.0 -1.7 FIG005080: Possible exported protein compare
Pf6N2E2_5337 -1.0 -8.0 Ubiquinol-cytochrome C reductase iron-sulfur subunit (EC 1.10.2.2) compare
Pf6N2E2_40 -1.0 -2.2 Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1) compare
Pf6N2E2_2253 -1.0 -1.3 hypothetical protein compare
Pf6N2E2_3666 -1.0 -0.7 InaA protein compare
Pf6N2E2_2213 -1.0 -3.7 Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1) compare
Pf6N2E2_2266 -1.0 -3.2 Copper-sensing two-component system response regulator CusR compare
Pf6N2E2_2065 -1.0 -1.3 Cobalt-precorrin-4 C11-methyltransferase (EC 2.1.1.133) compare
Pf6N2E2_1691 -1.0 -1.3 hypothetical protein compare
Pf6N2E2_2186 -1.0 -3.8 Isovaleryl-CoA dehydrogenase (EC 1.3.8.4) compare
Pf6N2E2_1506 -1.0 -1.9 Putative alkanesulfonate metabolism utilization regulator compare
Pf6N2E2_4298 -1.0 -2.4 Uncharacterized protein ImpB compare
Pf6N2E2_3257 -1.0 -1.9 Uncharacterized ABC transporter, auxiliary component YrbC compare
Pf6N2E2_2117 -1.0 -7.3 1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18) compare
Pf6N2E2_3340 -1.0 -6.9 Fructose repressor FruR, LacI family compare
Pf6N2E2_2823 -1.0 -3.7 Multidrug efflux RND membrane fusion protein MexC compare
Pf6N2E2_1116 -1.0 -1.0 Inosine-5'-monophosphate dehydrogenase (EC 1.1.1.205) compare
Pf6N2E2_5156 -1.0 -0.7 Two-component sensor CbrB: intrcellular carbon:nitrogen balance compare
Pf6N2E2_1534 -1.0 -1.3 Probable transmembrane protein compare
Pf6N2E2_1705 -0.9 -1.7 Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1) compare
Pf6N2E2_3698 -0.9 -2.8 Nicotinamidase family protein YcaC compare
Pf6N2E2_3373 -0.9 -2.6 Outer membrane lipoprotein SmpA, a component of the essential YaeT outer-membrane protein assembly complex compare
Pf6N2E2_2927 -0.9 -2.3 Putative signal peptide protein compare
Pf6N2E2_5884 -0.9 -5.6 Sensor histidine kinase compare
Pf6N2E2_4083 -0.9 -2.3 hypothetical protein compare
Pf6N2E2_2824 -0.9 -5.7 RND efflux system, inner membrane transporter CmeB compare
Pf6N2E2_4206 -0.9 -1.3 Chromosome (plasmid) partitioning protein ParB compare
Pf6N2E2_3151 -0.9 -3.7 Glutamate synthase [NADPH] small chain (EC 1.4.1.13) compare
Pf6N2E2_1490 -0.9 -1.9 hypothetical protein compare


Specific Phenotypes

For 24 genes in this experiment

For denitrifying Sodium pyruvate in Pseudomonas fluorescens FW300-N2E2

For denitrifying Sodium pyruvate across organisms