Experiment set16IT036 for Pseudomonas fluorescens FW300-N2E2

Compare to:

Nitrate reduction in presence of wild-type Pseudomonas N2-E3; replicate B

200 most important genes:

  gene name fitness t score description  
Pf6N2E2_477 -6.9 -4.8 Branched-chain amino acid aminotransferase (EC 2.6.1.42) compare
Pf6N2E2_4473 -5.8 -8.0 HlyD family secretion protein compare
Pf6N2E2_3842 -5.8 -4.0 Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19) compare
Pf6N2E2_4826 -5.8 -5.7 Indole-3-glycerol phosphate synthase (EC 4.1.1.48) compare
Pf6N2E2_3782 -5.7 -3.9 Phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19) compare
Pf6N2E2_736 -5.7 -3.9 hypothetical protein compare
Pf6N2E2_3839 -5.7 -3.9 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) (from data) compare
Pf6N2E2_725 -5.6 -5.5 Nitrate/nitrite response regulator protein compare
Pf6N2E2_4825 -5.5 -6.6 Anthranilate phosphoribosyltransferase (EC 2.4.2.18) compare
Pf6N2E2_3253 -5.4 -3.7 ATP phosphoribosyltransferase (EC 2.4.2.17) (from data) compare
Pf6N2E2_5239 -5.4 -9.7 Putative sulfate permease conserved
Pf6N2E2_5177 -5.3 -9.5 Ketol-acid reductoisomerase (EC 1.1.1.86) compare
Pf6N2E2_64 -5.2 -4.6 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33) compare
Pf6N2E2_5394 -5.2 -5.1 Molybdenum cofactor biosynthesis protein MoaE compare
Pf6N2E2_432 -5.1 -6.1 Molybdenum ABC transporter, periplasmic molybdenum-binding protein ModA (TC 3.A.1.8.1) compare
Pf6N2E2_3630 -5.1 -6.0 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf6N2E2_3610 -5.0 -3.5 ATP phosphoribosyltransferase regulatory subunit (EC 2.4.2.17) compare
Pf6N2E2_3751 -5.0 -12.1 Glutamate synthase [NADPH] small chain (EC 1.4.1.13) compare
Pf6N2E2_3783 -5.0 -4.9 phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31) (from data) compare
Pf6N2E2_4564 -5.0 -9.5 Dihydroxy-acid dehydratase (EC 4.2.1.9) compare
Pf6N2E2_2323 -5.0 -3.4 Fumarate and nitrate reduction regulatory protein compare
Pf6N2E2_4472 -4.9 -16.6 ABC-type multidrug transport system, permease component compare
Pf6N2E2_4597 -4.8 -6.6 Homoserine O-acetyltransferase (EC 2.3.1.31) compare
Pf6N2E2_723 -4.8 -5.3 FIG138517: Putative lipid carrier protein compare
Pf6N2E2_3645 -4.7 -13.3 Type I secretion outer membrane protein, TolC precursor compare
Pf6N2E2_66 -4.6 -9.3 3-isopropylmalate dehydrogenase (EC 1.1.1.85) compare
Pf6N2E2_726 -4.6 -6.2 Nitrate/nitrite sensor protein (EC 2.7.3.-) compare
Pf6N2E2_4596 -4.5 -9.2 Homoserine O-acetyltransferase (EC 2.3.1.31) compare
Pf6N2E2_3487 -4.5 -10.5 Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4) compare
Pf6N2E2_729 -4.5 -15.0 Respiratory nitrate reductase alpha chain (EC 1.7.99.4) conserved
Pf6N2E2_3932 -4.5 -7.3 D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95) compare
Pf6N2E2_5223 -4.5 -6.8 Molybdopterin biosynthesis protein MoeB compare
Pf6N2E2_732 -4.5 -6.1 Respiratory nitrate reductase gamma chain (EC 1.7.99.4) compare
Pf6N2E2_722 -4.5 -6.8 FIG139928: Putative protease compare
Pf6N2E2_3752 -4.4 -21.7 Glutamate synthase [NADPH] large chain (EC 1.4.1.13) compare
Pf6N2E2_730 -4.4 -8.5 Respiratory nitrate reductase beta chain (EC 1.7.99.4) compare
Pf6N2E2_3252 -4.4 -7.9 Histidinol dehydrogenase (EC 1.1.1.23) compare
Pf6N2E2_718 -4.3 -5.0 Pyridoxamine 5'-phosphate oxidase (EC 1.4.3.5) compare
Pf6N2E2_728 -4.2 -4.1 Nitrate/nitrite transporter compare
Pf6N2E2_5258 -4.1 -9.2 Sulfate adenylyltransferase subunit 1 (EC 2.7.7.4) / Adenylylsulfate kinase (EC 2.7.1.25) compare
Pf6N2E2_77 -4.1 -4.0 O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48) compare
Pf6N2E2_4047 -4.0 -3.9 Tryptophan synthase alpha chain (EC 4.2.1.20) compare
Pf6N2E2_5392 -4.0 -2.8 Molybdenum cofactor biosynthesis protein MoaC compare
Pf6N2E2_731 -4.0 -6.2 Respiratory nitrate reductase delta chain (EC 1.7.99.4) compare
Pf6N2E2_5309 -4.0 -8.0 Permease of the drug/metabolite transporter (DMT) superfamily compare
Pf6N2E2_735 -3.9 -14.9 Ribonucleotide reductase of class III (anaerobic), large subunit (EC 1.17.4.2) conserved
Pf6N2E2_4351 -3.9 -14.9 Predicted signal transduction protein compare
Pf6N2E2_71 -3.9 -7.4 Phosphoribosylanthranilate isomerase (EC 5.3.1.24) compare
Pf6N2E2_711 -3.9 -8.9 Nitric oxide reductase activation protein NorD conserved
Pf6N2E2_1296 -3.9 -6.9 DNA-binding response regulator, LuxR family compare
Pf6N2E2_3841 -3.7 -4.4 Imidazole glycerol phosphate synthase amidotransferase subunit (EC 2.4.2.-) compare
Pf6N2E2_4048 -3.6 -6.4 Tryptophan synthase beta chain (EC 4.2.1.20) compare
Pf6N2E2_4471 -3.5 -9.3 ABC-type multidrug transport system, permease component compare
Pf6N2E2_1293 -3.5 -4.1 COG2879, Hypothetical small protein yjiX compare
Pf6N2E2_3300 -3.5 -3.3 Predicted D-glucarate or D-galactorate regulator, GntR family compare
Pf6N2E2_2074 -3.4 -2.4 required for sulfate utilization, putative electron transport protein for sulfite reductase (from data) compare
Pf6N2E2_721 -3.4 -9.0 FIG139552: Putative protease compare
Pf6N2E2_4921 -3.4 -4.6 3,4-dihydroxy-2-butanone 4-phosphate synthase (EC 4.1.99.12) / GTP cyclohydrolase II (EC 3.5.4.25) compare
Pf6N2E2_760 -3.4 -15.2 Na+/H+ antiporter NhaB compare
Pf6N2E2_63 -3.4 -3.3 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33) compare
Pf6N2E2_2277 -3.3 -3.2 NADPH-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.13) compare
Pf6N2E2_2218 -3.3 -3.2 ATP-dependent Clp protease proteolytic subunit (EC 3.4.21.92) compare
Pf6N2E2_2217 -3.3 -2.2 ATP-dependent Clp protease ATP-binding subunit ClpX compare
Pf6N2E2_5242 -3.2 -2.2 Malate:quinone oxidoreductase (EC 1.1.5.4) compare
Pf6N2E2_5257 -3.2 -9.5 Sulfate adenylyltransferase subunit 2 (EC 2.7.7.4) compare
Pf6N2E2_3255 -3.2 -4.3 YrbA protein compare
Pf6N2E2_2465 -3.1 -5.9 Cys regulon transcriptional activator CysB compare
Pf6N2E2_737 -3.1 -2.1 Ribonucleotide reductase of class III (anaerobic), activating protein (EC 1.97.1.4) compare
Pf6N2E2_3346 -3.0 -6.7 Quinolinate phosphoribosyltransferase [decarboxylating] (EC 2.4.2.19) compare
Pf6N2E2_2073 -3.0 -6.2 Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2) compare
Pf6N2E2_3264 -2.9 -5.0 Uncharacterized protein YrbK clustered with lipopolysaccharide transporters compare
Pf6N2E2_2189 -2.9 -3.4 Hydroxymethylglutaryl-CoA lyase (EC 4.1.3.4) compare
Pf6N2E2_5080 -2.9 -11.1 Putative metal chaperone, involved in Zn homeostasis, GTPase of COG0523 family compare
Pf6N2E2_431 -2.8 -6.2 Molybdenum transport system permease protein ModB (TC 3.A.1.8.1) compare
Pf6N2E2_2422 -2.8 -9.0 Molybdopterin biosynthesis protein MoeA compare
Pf6N2E2_5666 -2.8 -11.2 Arginine N-succinyltransferase, alpha subunit (EC 2.3.1.109) compare
Pf6N2E2_5175 -2.7 -5.5 Acetolactate synthase large subunit (EC 2.2.1.6) compare
Pf6N2E2_5299 -2.7 -9.7 Benzoate transport protein compare
Pf6N2E2_5520 -2.7 -6.7 Sensory box histidine kinase compare
Pf6N2E2_4813 -2.7 -13.7 Probable two-component sensor, near polyamine transporter compare
Pf6N2E2_2574 -2.7 -1.8 Flagellar synthesis regulator FleN compare
Pf6N2E2_4797 -2.6 -2.5 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase (EC 2.7.6.3) compare
Pf6N2E2_1411 -2.6 -7.5 Coproporphyrinogen III oxidase, oxygen-independent (EC 1.3.99.22) compare
Pf6N2E2_5728 -2.6 -3.3 Phosphoribosylglycinamide formyltransferase (EC 2.1.2.2) compare
Pf6N2E2_3074 -2.6 -1.4 FIG00960973: hypothetical protein compare
Pf6N2E2_1412 -2.6 -3.4 hypothetical protein compare
Pf6N2E2_1294 -2.6 -2.6 Carbon starvation protein A compare
Pf6N2E2_289 -2.5 -13.4 Isocitrate dehydrogenase [NADP] (EC 1.1.1.42) compare
Pf6N2E2_2447 -2.5 -6.3 Probable protease htpX homolog (EC 3.4.24.-) compare
Pf6N2E2_3170 -2.5 -5.9 2-isopropylmalate synthase (EC 2.3.3.13) compare
Pf6N2E2_2389 -2.4 -9.4 Phosphoenolpyruvate synthase (EC 2.7.9.2) compare
Pf6N2E2_3282 -2.4 -1.7 FIG005080: Possible exported protein compare
Pf6N2E2_4049 -2.4 -8.8 Glycine cleavage system transcriptional activator compare
Pf6N2E2_292 -2.4 -9.9 ATP-dependent Clp protease ATP-binding subunit ClpA compare
Pf6N2E2_3940 -2.4 -10.3 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf6N2E2_5328 -2.3 -6.1 FIG00958649: hypothetical protein compare
Pf6N2E2_5308 -2.3 -11.4 Transcriptional regulator, AraC family compare
Pf6N2E2_2424 -2.3 -2.4 Molybdopterin-guanine dinucleotide biosynthesis protein MobA compare
Pf6N2E2_5553 -2.3 -7.1 Quinolinate synthetase (EC 2.5.1.72) compare
Pf6N2E2_4638 -2.3 -2.2 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20) compare
Pf6N2E2_5176 -2.3 -4.7 Acetolactate synthase small subunit (EC 2.2.1.6) compare
Pf6N2E2_3847 -2.3 -2.2 FIG001341: Probable Fe(2+)-trafficking protein YggX compare
Pf6N2E2_733 -2.2 -2.9 Peptidyl-prolyl cis-trans isomerase PpiD (EC 5.2.1.8) compare
Pf6N2E2_156 -2.2 -3.2 Carbon storage regulator compare
Pf6N2E2_4372 -2.2 -4.1 FIG00460773: hypothetical protein compare
Pf6N2E2_3611 -2.1 -1.9 HflC protein compare
Pf6N2E2_2508 -2.1 -3.7 hypothetical protein compare
Pf6N2E2_2506 -2.1 -9.8 nucleotide sugar epimerase/dehydratase WbpM compare
Pf6N2E2_5990 -2.1 -1.9 Translation initiation factor 2 (IF-2; GTPase) compare
Pf6N2E2_3807 -2.1 -4.8 Histidine utilization repressor compare
Pf6N2E2_5295 -2.0 -5.3 Serine hydroxymethyltransferase (EC 2.1.2.1) compare
Pf6N2E2_3984 -2.0 -11.4 T1SS associated transglutaminase-like cysteine proteinase LapP compare
Pf6N2E2_706 -2.0 -5.5 Nitric oxide reductase activation protein NorQ compare
Pf6N2E2_4187 -2.0 -6.8 Lipid A biosynthesis lauroyl acyltransferase (EC 2.3.1.-) compare
Pf6N2E2_2200 -1.9 -1.8 RNA polymerase sigma-54 factor RpoN compare
Pf6N2E2_3204 -1.9 -2.2 Rho-specific inhibitor of transcription termination (YaeO) compare
Pf6N2E2_4607 -1.9 -4.1 Uracil phosphoribosyltransferase (EC 2.4.2.9) / Pyrimidine operon regulatory protein PyrR compare
Pf6N2E2_4454 -1.9 -5.8 Exopolyphosphatase (EC 3.6.1.11) compare
Pf6N2E2_5262 -1.8 -2.5 FKBP-type peptidyl-prolyl cis-trans isomerase SlyD (EC 5.2.1.8) compare
Pf6N2E2_2507 -1.8 -6.6 Undecaprenyl-phosphate N-acetylglucosaminyl 1-phosphate transferase (EC 2.7.8.-) compare
Pf6N2E2_5884 -1.8 -8.9 Sensor histidine kinase compare
Pf6N2E2_717 -1.8 -7.0 Functional role page for Anaerobic nitric oxide reductase transcription regulator NorR compare
Pf6N2E2_4612 -1.8 -3.4 Glutathione synthetase (EC 6.3.2.3) compare
Pf6N2E2_2825 -1.7 -5.5 RND efflux system, outer membrane lipoprotein CmeC compare
Pf6N2E2_1295 -1.7 -4.5 Sensor histidine kinase compare
Pf6N2E2_2181 -1.7 -3.5 Arginine N-succinyltransferase (EC 2.3.1.109) compare
Pf6N2E2_2822 -1.7 -6.1 Transcription repressor of multidrug efflux pump acrAB operon, TetR (AcrR) family compare
Pf6N2E2_2824 -1.7 -7.8 RND efflux system, inner membrane transporter CmeB compare
Pf6N2E2_4817 -1.7 -12.5 Spermidine Putrescine ABC transporter permease component PotB (TC 3.A.1.11.1) compare
Pf6N2E2_3658 -1.7 -4.8 Lipid A core - O-antigen ligase and related enzymes compare
Pf6N2E2_3248 -1.7 -1.9 Probable transmembrane protein compare
Pf6N2E2_4803 -1.7 -1.1 Bis(5'-nucleosyl)-tetraphosphatase, symmetrical (EC 3.6.1.41) compare
Pf6N2E2_4818 -1.6 -5.3 Spermidine Putrescine ABC transporter permease component potC (TC_3.A.1.11.1) compare
Pf6N2E2_5679 -1.6 -3.8 hypothetical protein compare
Pf6N2E2_3039 -1.6 -7.5 Protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77) compare
Pf6N2E2_5665 -1.6 -6.0 Succinylornithine transaminase (EC 2.6.1.81) compare
Pf6N2E2_2201 -1.6 -2.9 Transmembrane regulator protein PrtR compare
Pf6N2E2_4273 -1.6 -6.4 ATP-dependent DNA helicase UvrD/PcrA compare
Pf6N2E2_2509 -1.5 -2.7 UDP-glucose 4-epimerase (EC 5.1.3.2) compare
Pf6N2E2_2681 -1.5 -1.0 3-phosphoglycerate kinase compare
Pf6N2E2_2823 -1.5 -5.1 Multidrug efflux RND membrane fusion protein MexC compare
Pf6N2E2_714 -1.5 -5.0 NnrS protein involved in response to NO compare
Pf6N2E2_1490 -1.5 -2.3 hypothetical protein compare
Pf6N2E2_5111 -1.5 -2.6 FKBP-type peptidyl-prolyl cis-trans isomerase SlpA (EC 5.2.1.8) compare
Pf6N2E2_3340 -1.5 -8.8 Fructose repressor FruR, LacI family compare
Pf6N2E2_709 -1.5 -6.4 Nitric-oxide reductase subunit C (EC 1.7.99.7) compare
Pf6N2E2_4097 -1.5 -10.3 FIG00953365: hypothetical protein compare
Pf6N2E2_291 -1.5 -4.8 ATP-dependent Clp protease adaptor protein ClpS compare
Pf6N2E2_2510 -1.4 -1.9 Lipid carrier : UDP-N-acetylgalactosaminyltransferase (EC 2.4.1.-) / Alpha-1,3-N-acetylgalactosamine transferase PglA (EC 2.4.1.-); Putative glycosyltransferase compare
Pf6N2E2_4414 -1.4 -1.3 FIG00953808: hypothetical protein compare
Pf6N2E2_658 -1.4 -8.2 sensor histidine kinase compare
Pf6N2E2_4993 -1.4 -2.8 Gamma-glutamyl phosphate reductase (EC 1.2.1.41) compare
Pf6N2E2_2012 -1.4 -1.9 hypothetical protein compare
Pf6N2E2_1932 -1.4 -2.1 Transcriptional regulator, IclR family compare
Pf6N2E2_727 -1.4 -4.1 Nitrate/nitrite transporter compare
Pf6N2E2_5894 -1.4 -6.3 Putative threonine efflux protein compare
Pf6N2E2_5843 -1.4 -2.2 hypothetical protein compare
Pf6N2E2_3251 -1.4 -7.4 histidinol-phosphate aminotransferase (EC 2.6.1.9) (from data) compare
Pf6N2E2_4422 -1.4 -2.6 Frataxin homolog CyaY, facilitates iron supply for heme A synthesis or Fe-S cluster assembly compare
Pf6N2E2_3763 -1.4 -1.5 hypothetical protein compare
Pf6N2E2_3027 -1.3 -0.9 Baseplate assembly protein V compare
Pf6N2E2_2180 -1.3 -4.3 Arginine N-succinyltransferase, alpha subunit (EC 2.3.1.109) compare
Pf6N2E2_863 -1.3 -5.6 Transcriptional regulator, IclR family compare
Pf6N2E2_712 -1.3 -2.7 hypothetical protein compare
Pf6N2E2_5471 -1.3 -5.7 Transcriptional activator MetR compare
Pf6N2E2_1673 -1.3 -1.3 Transcriptional regulator, TetR family compare
Pf6N2E2_3521 -1.3 -7.1 Sensor histidine kinase/response regulator compare
Pf6N2E2_2513 -1.3 -1.5 UDP-glucose dehydrogenase (EC 1.1.1.22) compare
Pf6N2E2_6099 -1.3 -0.9 tRNA-Glu-TTC compare
Pf6N2E2_1072 -1.3 -4.0 LmbE-like protein compare
Pf6N2E2_5950 -1.3 -3.2 Phosphate:acyl-ACP acyltransferase PlsX compare
Pf6N2E2_2752 -1.3 -0.9 Exported zinc metalloprotease YfgC precursor compare
Pf6N2E2_5298 -1.3 -3.9 Sarcosine oxidase beta subunit (EC 1.5.3.1) compare
Pf6N2E2_3908 -1.3 -0.9 Glutamate--cysteine ligase (EC 6.3.2.2) compare
Pf6N2E2_2986 -1.3 -2.9 Transcriptional regulator, LuxR family compare
Pf6N2E2_895 -1.3 -3.2 Transcriptional regulator, HxlR family compare
Pf6N2E2_1043 -1.3 -2.5 Transcriptional regulator, IclR family compare
Pf6N2E2_4181 -1.2 -0.8 Organic hydroperoxide resistance protein compare
Pf6N2E2_193 -1.2 -3.0 hypothetical protein compare
Pf6N2E2_2186 -1.2 -4.3 Isovaleryl-CoA dehydrogenase (EC 1.3.8.4) compare
Pf6N2E2_264 -1.2 -2.7 Transcriptional regulator, TetR family compare
Pf6N2E2_2758 -1.2 -8.9 L-aspartate oxidase (EC 1.4.3.16) compare
Pf6N2E2_3134 -1.2 -1.2 hypothetical protein compare
Pf6N2E2_3948 -1.2 -7.4 Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4) compare
Pf6N2E2_302 -1.2 -3.2 Siroheme synthase / Precorrin-2 oxidase (EC 1.3.1.76) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4) / Uroporphyrinogen-III methyltransferase (EC 2.1.1.107) compare
Pf6N2E2_510 -1.2 -7.2 Predicted transcriptional regulator of the myo-inositol catabolic operon compare
Pf6N2E2_5899 -1.2 -5.7 Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system compare
Pf6N2E2_434 -1.2 -2.4 hypothetical protein compare
Pf6N2E2_4298 -1.2 -3.3 Uncharacterized protein ImpB compare
Pf6N2E2_2423 -1.2 -3.1 Molybdenum cofactor biosynthesis protein MoaB compare
Pf6N2E2_4987 -1.2 -1.4 MaoC-like domain protein compare
Pf6N2E2_3659 -1.2 -2.5 Protein fixF compare
Pf6N2E2_710 -1.2 -5.8 Nitric-oxide reductase subunit B (EC 1.7.99.7) conserved
Pf6N2E2_3933 -1.1 -5.8 D-2-hydroxyglutarate dehydrogenase compare
Pf6N2E2_5014 -1.1 -0.5 Apolipoprotein N-acyltransferase (EC 2.3.1.-) / Copper homeostasis protein CutE compare
Pf6N2E2_2865 -1.1 -7.1 Gluconate utilization system Gnt-I transcriptional repressor compare
Pf6N2E2_4360 -1.1 -6.3 Bona fide RidA/YjgF/TdcF/RutC subgroup compare
Pf6N2E2_3206 -1.1 -1.2 hypothetical protein compare
Pf6N2E2_5740 -1.1 -2.2 Adenosylcobinamide-phosphate synthase (EC 6.3.1.10) compare
Pf6N2E2_285 -1.1 -2.6 FIG002903: a protein of unknown function perhaps involved in purine metabolism compare


Specific Phenotypes

For 28 genes in this experiment

For denitrifying Sodium pyruvate in Pseudomonas fluorescens FW300-N2E2

For denitrifying Sodium pyruvate across organisms