Experiment set15IT067 for Pseudomonas fluorescens SBW25-INTG

Glucose (C) and L-Glutamine (N); with MOPS; with Sodium chloride

Group: stress
Media: MME_noNitrogen_noCarbon + D-Glucose (10 mM) + L-Glutamine (10 mM) + Sodium Chloride (400 mM), pH=7
Culturing: PseudoSBW25_INTG_ML3, 96 deep-well microplate; 1.2 mL volume, Aerobic, at 8 (C), shaken=1200 rpm
By: Joshua Elmore on September 1, 2021
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)

Specific Phenotypes

For 5 genes in this experiment

For stress D-Glucose in Pseudomonas fluorescens SBW25-INTG

For stress D-Glucose across organisms

SEED Subsystems

Subsystem	#Specific
2-Ketogluconate Utilization	1
Arginine and Ornithine Degradation	1
CBSS-262719.3.peg.410	1
Glutamate dehydrogenases	1
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis	1
Proline Synthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pentose phosphate pathway
• Purine metabolism
• Glutamate metabolism
• Arginine and proline metabolism
• Glutathione metabolism
• Lipopolysaccharide biosynthesis
• Folate biosynthesis
• Nitrogen metabolism
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-glutamate biosynthesis III	1	1	1
glucose degradation (oxidative)	5	5	1
L-glutamate and L-glutamine biosynthesis	7	6	1