Experiment set15IT064 for Paraburkholderia graminis OAS925

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Plant=no_plant; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_liquid; Collection=outgrowth_R2A_with_Tween_and_Cycloheximide; Time=21_days

Group: in planta
Media: + Plant=no_plant; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_liquid; Collection=outgrowth_R2A_with_Tween_and_Cycloheximide; Time=21_days
Culturing: Burkholderia_OAS925_ML2 (AMD1412), pot, at 26 (C), (Solid)
By: Marta Torres on 18-Apr-22

Specific Phenotypes

For 43 genes in this experiment

For in planta Plant=no_plant; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_liquid; Collection=outgrowth_R2A_with_Tween_and_Cycloheximide; Time=21_days in Paraburkholderia graminis OAS925

For in planta Plant=no_plant; PlantTreatment=None; Sample=soil; GrowthSubstrate=0.5X_MS_liquid; Collection=outgrowth_R2A_with_Tween_and_Cycloheximide; Time=21_days across organisms

SEED Subsystems

Subsystem #Specific
Polyamine Metabolism 5
DNA-replication 4
Acetyl-CoA fermentation to Butyrate 1
Bacterial hemoglobins 1
Butanol Biosynthesis 1
Cobalt-zinc-cadmium resistance 1
Coenzyme B12 biosynthesis 1
Colanic acid biosynthesis 1
DNA Repair Base Excision 1
DNA repair, bacterial RecFOR pathway 1
Folate Biosynthesis 1
Isoleucine degradation 1
Lacto-N-Biose I and Galacto-N-Biose Metabolic Pathway 1
Lactose and Galactose Uptake and Utilization 1
Multidrug Resistance Efflux Pumps 1
N-linked Glycosylation in Bacteria 1
Polyhydroxybutyrate metabolism 1
Proline, 4-hydroxyproline uptake and utilization 1
Protein degradation 1
Rhamnose containing glycans 1
Soluble cytochromes and functionally related electron carriers 1
Valine degradation 1
ZZ gjo need homes 1
linker unit-arabinogalactan synthesis 1
n-Phenylalkanoic acid degradation 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
UDP-α-D-galactose biosynthesis 1 1 1
UDP-N-acetyl-D-galactosamine biosynthesis I 1 1 1
L-glutamine degradation I 1 1 1
fatty acid β-oxidation III (unsaturated, odd number) 1 1 1
L-asparagine biosynthesis III (tRNA-dependent) 4 4 3
glutaminyl-tRNAgln biosynthesis via transamidation 4 3 3
benzoyl-CoA biosynthesis 3 3 2
L-glutamate biosynthesis I 2 2 1
UDP-α-D-galactofuranose biosynthesis 2 1 1
oleate β-oxidation (thioesterase-dependent, yeast) 2 1 1
oleate β-oxidation 35 29 16
fatty acid β-oxidation I (generic) 7 5 3
adipate degradation 5 5 2
adipate biosynthesis 5 4 2
fatty acid β-oxidation II (plant peroxisome) 5 3 2
glutaryl-CoA degradation 5 3 2
fatty acid β-oxidation IV (unsaturated, even number) 5 3 2
fatty acid β-oxidation V (unsaturated, odd number, di-isomerase-dependent) 5 2 2
pyruvate fermentation to hexanol (engineered) 11 7 4
(8E,10E)-dodeca-8,10-dienol biosynthesis 11 6 4
2-methyl-branched fatty acid β-oxidation 14 9 5
ammonia assimilation cycle III 3 3 1
fatty acid salvage 6 5 2
valproate β-oxidation 9 6 3
L-isoleucine degradation I 6 4 2
pyruvate fermentation to butanol II (engineered) 6 4 2
2-chloroacrylate degradation I 3 2 1
methyl ketone biosynthesis (engineered) 6 3 2
propanoate fermentation to 2-methylbutanoate 6 3 2
oleate β-oxidation (reductase-dependent, yeast) 3 1 1
D-galactose detoxification 3 1 1
benzoyl-CoA degradation I (aerobic) 7 5 2
Arg/N-end rule pathway (eukaryotic) 14 8 4
pyruvate fermentation to butanoate 7 4 2
fatty acid β-oxidation VI (mammalian peroxisome) 7 3 2
L-valine degradation I 8 5 2
pyruvate fermentation to butanol I 8 3 2
Salmonella enterica serotype O:8 O antigen biosynthesis 8 2 2
oleate β-oxidation (isomerase-dependent, yeast) 4 1 1
phenylacetate degradation I (aerobic) 9 7 2
superpathway of Clostridium acetobutylicum acidogenic fermentation 9 6 2
benzoate biosynthesis I (CoA-dependent, β-oxidative) 9 5 2
4-hydroxybenzoate biosynthesis III (plants) 5 4 1
4-chlorocatechol degradation 5 4 1
(R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) 5 3 1
D-galactose degradation I (Leloir pathway) 5 3 1
3-chlorocatechol degradation II (ortho) 5 3 1
L-glutamate degradation V (via hydroxyglutarate) 10 5 2
3-phenylpropanoate degradation 10 5 2
9-cis, 11-trans-octadecadienoyl-CoA degradation (isomerase-dependent, yeast) 10 4 2
3-chlorocatechol degradation I (ortho) 5 2 1
benzoate biosynthesis III (CoA-dependent, non-β-oxidative) 5 2 1
Salmonella enterica serotype O:3,10 O antigen biosynthesis 5 1 1
superpathway of phenylethylamine degradation 11 7 2
superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis 6 4 1
6-gingerol analog biosynthesis (engineered) 6 3 1
L-glutamate degradation VII (to butanoate) 12 5 2
methylthiopropanoate degradation I (cleavage) 6 2 1
Salmonella enterica serotype O:2 O antigen biosynthesis 6 1 1
Salmonella enterica serotype O:4 O antigen biosynthesis (group B1) 6 1 1
Salmonella enterica serotype O:9,46 O antigen biosynthesis 6 1 1
Salmonella enterica serotype O:9 O antigen biosynthesis 6 1 1
Porphyromonas gingivalis O-LPS antigen biosynthesis 6 1 1
superpathway of Clostridium acetobutylicum solventogenic fermentation 13 6 2
L-glutamate and L-glutamine biosynthesis 7 7 1
superpathway of glyoxylate cycle and fatty acid degradation 14 11 2
UDP-N-acetyl-D-galactosamine biosynthesis II 7 5 1
4,5-dichlorocatechol degradation 7 5 1
pyrimidine deoxyribonucleotides de novo biosynthesis II 7 5 1
stachyose degradation 7 2 1
Spodoptera littoralis pheromone biosynthesis 22 4 3
L-tryptophan degradation III (eukaryotic) 15 6 2
L-citrulline biosynthesis 8 7 1
glycerol degradation to butanol 16 10 2
3,5-dichlorocatechol degradation 8 5 1
crotonate fermentation (to acetate and cyclohexane carboxylate) 16 5 2
2-methylpropene degradation 8 2 1
superpathway of dimethylsulfoniopropanoate degradation 8 2 1
superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation 17 9 2
benzoate fermentation (to acetate and cyclohexane carboxylate) 17 6 2
3,4,6-trichlorocatechol degradation 9 7 1
3-hydroxypropanoate/4-hydroxybutanate cycle 18 10 2
1,4-dichlorobenzene degradation 9 5 1
UDP-sugars interconversion 9 3 1
toluene degradation VI (anaerobic) 18 4 2
teichuronic acid biosynthesis (B. subtilis 168) 9 2 1
Salmonella enterica serotype O:9,46,27 O antigen biosynthesis 9 1 1
methyl tert-butyl ether degradation 10 3 1
colanic acid building blocks biosynthesis 11 11 1
gallate degradation III (anaerobic) 11 5 1
superpathway of L-citrulline metabolism 12 9 1
10-trans-heptadecenoyl-CoA degradation (reductase-dependent, yeast) 12 2 1
10-cis-heptadecenoyl-CoA degradation (yeast) 12 2 1
androstenedione degradation I (aerobic) 25 6 2
platensimycin biosynthesis 26 6 2
(4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) 13 2 1
1-butanol autotrophic biosynthesis (engineered) 27 20 2
androstenedione degradation II (anaerobic) 27 4 2
superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) 14 12 1
superpathway of cholesterol degradation I (cholesterol oxidase) 42 9 3
superpathway of testosterone and androsterone degradation 28 6 2
docosahexaenoate biosynthesis III (6-desaturase, mammals) 14 2 1
succinoglycan biosynthesis 14 1 1
superpathway of cholesterol degradation II (cholesterol dehydrogenase) 47 9 3
cholesterol degradation to androstenedione I (cholesterol oxidase) 17 3 1
mycolyl-arabinogalactan-peptidoglycan complex biosynthesis 18 1 1
cholesterol degradation to androstenedione II (cholesterol dehydrogenase) 22 3 1
superpathway of cholesterol degradation III (oxidase) 49 5 2
photosynthetic 3-hydroxybutanoate biosynthesis (engineered) 26 19 1
superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis 33 12 1