Experiment set15IT058 for Desulfovibrio vulgaris Hildenborough JW710
MoLS4 with L-Valine (HPLC) 20mM nitrogen source
Group: nitrogen sourceMedia: MoLS4_no_ammonium + L-Valine (HPLC purified) (20 mM), pH=7.2
Culturing: DvH_JW710, 24 deep-well microplate, Anaerobic, at 30 (C), shaken=0 rpm
By: Valentine on 10/26/2017
Media components: 30 mM Sodium sulfate, 60 mM Sodium D,L-Lactate, 30 mM Tris hydrochloride, 0.12 mM EDTA, 1 mM Sodium sulfide nonahydrate, 8 mM Magnesium chloride hexahydrate, 0.6 mM Calcium chloride, 2 mM Potassium phosphate dibasic, 60 uM Iron (II) chloride tetrahydrate, Desulfovibrio trace elements (15 uM Manganese (II) chloride tetrahydrate, 7.8 uM Cobalt chloride hexahydrate, 9 uM Zinc chloride, 1.26 uM Sodium molybdate, 1.92 uM Boric Acid, 2.28 uM Nickel (II) sulfate hexahydrate, 0.06 uM copper (II) chloride dihydrate, 0.21 uM Sodium selenite pentahydrate, 0.144 uM Sodium tungstate dihydrate), Thauer's vitamin mix (0.01 mg/L Pyridoxine HCl, 0.005 mg/L 4-Aminobenzoic acid, 0.005 mg/L Lipoic acid, 0.005 mg/L Nicotinic Acid, 0.005 mg/L Riboflavin, 0.005 mg/L Thiamine HCl, 0.005 mg/L calcium pantothenate, 0.002 mg/L biotin, 0.002 mg/L Folic Acid, 0.0001 mg/L Cyanocobalamin, 0.2 mg/L Choline chloride)
Specific Phenotypes
For 1 genes in this experiment
For nitrogen source L-Valine (HPLC purified) in Desulfovibrio vulgaris Hildenborough JW710
For nitrogen source L-Valine (HPLC purified) across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Acetyl-CoA fermentation to Butyrate | 1 |
| Isoleucine degradation | 1 |
| Valine degradation | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| (S)-propane-1,2-diol degradation | 5 | 3 | 1 |
| L-threonine degradation I | 6 | 5 | 1 |
| pyruvate fermentation to butanoate | 7 | 3 | 1 |
| superpathway of Clostridium acetobutylicum acidogenic fermentation | 9 | 5 | 1 |
| L-glutamate degradation VII (to butanoate) | 12 | 4 | 1 |
| superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation | 17 | 8 | 1 |
| superpathway of L-threonine metabolism | 18 | 13 | 1 |