Experiment set15IT055 for Pseudomonas fluorescens SBW25-INTG

Compare to:

Betaine (C)(N); with MOPS

Group: no stress control
Media: MME_noNitrogen_noCarbon + Betaine (10 mM) + Betaine (10 mM), pH=7
Culturing: PseudoSBW25_INTG_ML3, 96 deep-well microplate; 1.2 mL volume, Aerobic, at 33 (C), shaken=1200 rpm
By: Joshua Elmore on September 1, 2021
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)

Specific Phenotypes

For 2 genes in this experiment

For no stress control Betaine in Pseudomonas fluorescens SBW25-INTG

For no stress control Betaine across organisms

SEED Subsystems

Subsystem #Specific
Chorismate: Intermediate for synthesis of PAPA antibiotics, PABA, anthranilate, 3-hydroxyanthranilate and more. 1
Folate Biosynthesis 1
Molybdenum cofactor biosynthesis 1
Riboflavin, FMN and FAD metabolism 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-glutamine degradation I 1 1 1
L-glutamate biosynthesis I 2 2 1
4-aminobenzoate biosynthesis I 2 2 1
ammonia assimilation cycle III 3 3 1
L-asparagine biosynthesis III (tRNA-dependent) 4 4 1
glutaminyl-tRNAgln biosynthesis via transamidation 4 3 1
flavin biosynthesis I (bacteria and plants) 9 8 2
flavin biosynthesis III (fungi) 9 7 2
6-hydroxymethyl-dihydropterin diphosphate biosynthesis III (Chlamydia) 5 3 1
L-glutamate and L-glutamine biosynthesis 7 6 1
toxoflavin biosynthesis 7 3 1
L-citrulline biosynthesis 8 8 1
chloramphenicol biosynthesis 9 1 1
superpathway of tetrahydrofolate biosynthesis 10 8 1
flavin biosynthesis II (archaea) 10 5 1
superpathway of candicidin biosynthesis 11 4 1
superpathway of tetrahydrofolate biosynthesis and salvage 12 10 1
superpathway of L-citrulline metabolism 12 10 1
superpathway of chorismate metabolism 59 42 1