Experiment set15H33 for Pseudomonas stutzeri RCH2

RCH2 grown on a colony of ecoli

Group:
Media:
Culturing: psRCH2_ML7c
By: Peter Rademacher on

Specific Phenotypes

For 10 genes in this experiment

SEED Subsystems

Subsystem	#Specific
Leucine Degradation and HMG-CoA Metabolism	3
Serine-glyoxylate cycle	2
Aromatic amino acid interconversions with aryl acids	1
Branched-Chain Amino Acid Biosynthesis	1
Catechol branch of beta-ketoadipate pathway	1
D-gluconate and ketogluconates metabolism	1
Gentisare degradation	1
HMG CoA Synthesis	1
Protocatechuate branch of beta-ketoadipate pathway	1
Salicylate and gentisate catabolism	1
Transport of Iron	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Valine, leucine and isoleucine degradation
• Tyrosine metabolism
• Styrene degradation
• Butanoate metabolism
• Synthesis and degradation of ketone bodies
• Geraniol degradation
• Valine, leucine and isoleucine biosynthesis
• Glutathione metabolism
• 1- and 2-Methylnaphthalene degradation
• Benzoate degradation via CoA ligation
• Propanoate metabolism
• Metabolism of xenobiotics by cytochrome P450
• Drug metabolism - cytochrome P450

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
2-oxobutanoate degradation II	2	1	1
L-tyrosine degradation I	5	5	2
ketolysis	3	3	1
L-isoleucine biosynthesis V	3	3	1
L-leucine degradation I	6	5	2
L-isoleucine degradation III (oxidative Stickland reaction)	3	2	1
L-leucine degradation V (oxidative Stickland reaction)	3	2	1
L-valine degradation III (oxidative Stickland reaction)	3	2	1
4-hydroxy-2-nonenal detoxification	4	1	1
pentachlorophenol degradation	10	3	2
gallate degradation III (anaerobic)	11	5	2
L-isoleucine biosynthesis IV	6	5	1
L-glutamate degradation XI (reductive Stickland reaction)	7	4	1
acetyl-CoA fermentation to butanoate	7	4	1
4-aminobutanoate degradation V	7	3	1
succinate fermentation to butanoate	7	2	1
glutathione-mediated detoxification I	8	3	1
TCA cycle VI (Helicobacter)	9	7	1
gliotoxin biosynthesis	9	1	1
glutathione-mediated detoxification II	9	1	1
L-glutamate degradation V (via hydroxyglutarate)	10	7	1
indole glucosinolate activation (intact plant cell)	12	3	1
camalexin biosynthesis	12	2	1