Experiment set14S907 for Pantoea sp. MT58

Survival; Treatment=20250415-M4-SZ1-UF; TreatmentDuration=1week; Outgrowth=LB_Kan30

Group: survival
Media: + Treatment=20250415-M4-SZ1-UF; TreatmentDuration=1week; Outgrowth=LB_Kan30
Culturing: MT058_ML2, tube, Aerobic, at 30 (C)
By: Adams lab on 6/24/25

Specific Phenotypes

For 8 genes in this experiment

For survival Treatment=20250415-M4-SZ1-UF; TreatmentDuration=1week; Outgrowth=LB_Kan30 in Pantoea sp. MT58

For survival Treatment=20250415-M4-SZ1-UF; TreatmentDuration=1week; Outgrowth=LB_Kan30 across organisms

SEED Subsystems

Subsystem	#Specific
Glycerol and Glycerol-3-phosphate Uptake and Utilization	2
Acetyl-CoA fermentation to Butyrate	1
Anaerobic respiratory reductases	1
Biotin biosynthesis	1
Butanol Biosynthesis	1
D-Galacturonate and D-Glucuronate Utilization	1
Glycerol fermenation to 1,3-propanediol	1
Glycerolipid and Glycerophospholipid Metabolism in Bacteria	1
Isobutyryl-CoA to Propionyl-CoA Module	1
Isoleucine degradation	1
MLST	1
Valine degradation	1
n-Phenylalkanoic acid degradation	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Fatty acid metabolism
• Valine, leucine and isoleucine degradation
• Geraniol degradation
• Glycerolipid metabolism
• 1- and 2-Methylnaphthalene degradation
• Benzoate degradation via CoA ligation
• Butanoate metabolism
• Brassinosteroid biosynthesis
• Caprolactam degradation
• Biosynthesis of terpenoids and steroids
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
adenosine nucleotides degradation III	1	1	1
long-chain fatty acid activation	1	1	1
glycerol degradation I	3	3	2
glycerol and glycerophosphodiester degradation	4	4	2
arsenate detoxification III	2	2	1
γ-linolenate biosynthesis II (animals)	2	1	1
linoleate biosynthesis II (animals)	2	1	1
3-methyl-branched fatty acid α-oxidation	6	3	2
oleate biosynthesis I (plants)	3	1	1
alkane biosynthesis II	3	1	1
phytol degradation	4	3	1
long chain fatty acid ester synthesis (engineered)	4	2	1
phosphatidylcholine acyl editing	4	2	1
wax esters biosynthesis II	4	1	1
sporopollenin precursors biosynthesis	18	5	4
sphingosine and sphingosine-1-phosphate metabolism	10	4	2
octane oxidation	5	2	1
stearate biosynthesis II (bacteria and plants)	6	5	1
pyruvate fermentation to butanol II (engineered)	6	4	1
fatty acid salvage	6	4	1
arsenic detoxification (plants)	6	4	1
stearate biosynthesis IV	6	4	1
6-gingerol analog biosynthesis (engineered)	6	2	1
stearate biosynthesis I (animals)	6	1	1
fatty acid β-oxidation I (generic)	7	6	1
ceramide degradation by α-oxidation	7	2	1
icosapentaenoate biosynthesis II (6-desaturase, mammals)	7	1	1
capsaicin biosynthesis	7	1	1
arachidonate biosynthesis III (6-desaturase, mammals)	7	1	1
icosapentaenoate biosynthesis III (8-desaturase, mammals)	7	1	1
2-deoxy-D-ribose degradation II	8	3	1
ceramide and sphingolipid recycling and degradation (yeast)	16	4	2
oleate β-oxidation	35	29	4
suberin monomers biosynthesis	20	3	2
superpathway of fatty acid biosynthesis II (plant)	43	38	4
pyruvate fermentation to hexanol (engineered)	11	7	1
arsenic detoxification (yeast)	12	4	1
palmitate biosynthesis II (type II fatty acid synthase)	31	29	2
cutin biosynthesis	16	2	1
arsenic detoxification (mammals)	17	8	1
superpathway of fatty acids biosynthesis (E. coli)	53	51	2
1-butanol autotrophic biosynthesis (engineered)	27	19	1
palmitate biosynthesis III	29	21	1