Experiment set14S48 for Rhodanobacter denitrificans FW104-10B01

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NLDM_defined low iron; stationary

Group: nldm low iron
Media: NLDM_defined_no_iron + Iron (II) sulfate heptahydrate (0.05625 uM)
Culturing: rhodanobacter_10B01_ML12, tube, Aerobic, at 30 (C), shaken=200 rpm
By: Allison on 31-Jul-25
Media components: 1 mM Ammonium chloride, 3.3 mM Potassium Chloride, 0.812 mM Magnesium Sulfate Heptahydrate, 0.68 mM Calcium chloride dihydrate, 4.05 mM Disodium phosphate, 0.95 mM Sodium phosphate monobasic, ATCC Wolfe's mineral mix (5 mg/L EDTA, 3 mg/L Magnesium Sulfate Heptahydrate, 5 mg/L Manganese (II) sulfate monohydrate, 10 mg/L Sodium Chloride, 1 mg/L Iron (II) sulfate heptahydrate, 1 mg/L Cobalt(II) nitrate hexahydrate, 1 mg/L Calcium chloride dihydrate, 1 mg/L Zinc sulfate heptahydrate, 0.1 mg/L Copper (II) sulfate pentahydrate, 0.1 mg/L Aluminum potassium sulfate dodecahydrate, 0.1 mg/L Boric Acid, 0.1 mg/L Sodium Molybdate Dihydrate, 0.01 mg/L Sodium selenite pentahydrate, 0.1 mg/L Sodium tungstate dihydrate, 0.2 mg/L Nickel (II) chloride hexahydrate), ATCC Wolfe's mineral mix minus iron (5 mg/L EDTA, 3 mg/L Magnesium Sulfate Heptahydrate, 5 mg/L Manganese (II) sulfate monohydrate, 10 mg/L Sodium Chloride, 1 mg/L Cobalt(II) nitrate hexahydrate, 1 mg/L Calcium chloride dihydrate, 1 mg/L Zinc sulfate heptahydrate, 0.1 mg/L Copper (II) sulfate pentahydrate, 0.1 mg/L Aluminum potassium sulfate dodecahydrate, 0.1 mg/L Boric Acid, 0.1 mg/L Sodium Molybdate Dihydrate, 0.01 mg/L Sodium selenite pentahydrate, 0.1 mg/L Sodium tungstate dihydrate, 0.2 mg/L Nickel (II) chloride hexahydrate), NLDM_metabolites (875 uM Sucrose, 875 uM D-Glucose, 875 uM D-Trehalose dihydrate, 875 uM m-Inositol, 875 uM D-Xylose, 875 uM D-Mannitol, 875 uM N-Acetyl-D-Glucosamine, 525 uM Sodium L-Lactate, 525 uM D,L-Malic Acid, 525 uM Citric Acid, 525 uM Sodium succinate dibasic hexahydrate, 525 uM Sodium pyruvate, 525 uM a-Ketoglutaric acid disodium salt hydrate, 525 uM L-Citrulline, 175 uM L-Alanine, 175 uM L-Arginine, 175 uM L-Asparagine, 175 uM L-Aspartic Acid, 175 uM L-Cysteine, 175 uM L-Glutamic acid, 175 uM L-Glutamine, 175 uM Glycine, 175 uM L-Histidine, 175 uM L-Isoleucine, 175 uM L-Leucine, 175 uM L-Lysine, 175 uM L-Methionine, 175 uM L-Phenylalanine, 175 uM L-Proline, 175 uM L-Serine, 175 uM L-Threonine, 175 uM L-Tryptophan, 175 uM L-tyrosine, 175 uM L-Valine, 17.5 uM 5-methyluridine, 17.5 uM Adenine hydrochloride hydrate, 17.5 uM Adenosine, 17.5 uM Cytidine, 17.5 uM Cytosine, 17.5 uM Guanine, 17.5 uM Guanosine, 17.5 uM Hypoxanthine, 17.5 uM Inosine, 17.5 uM Thymine, 17.5 uM Uracil, 17.5 uM Uridine, 17.5 uM Xanthine, 17.5 uM Xanthosine, 17.5 uM Gamma-Aminobutyric Acid Hydrochloride, 17.5 uM Ectoine, 17.5 uM Betaine, 17.5 uM N-Acetyl-glutamic acid, 17.5 uM Nicotinamide, 17.5 uM L-Ornithine, 17.5 uM Shikimic Acid, 17.5 uM spermidine, 17.5 uM Taurine, 17.5 uM Trigonelline HCl, 17.5 uM Carnitine Hydrochloride, 17.5 uM Choline chloride, 17.5 uM n-Acetyl-glutamine, 17.5 uM n-Acetyl-lysine, 17.5 uM n-Acetyl-muramic acid, 17.5 uM sn-glycero-3-phosphocholine)

Specific Phenotypes

None in this experiment

For Rhodanobacter denitrificans FW104-10B01 in nldm low iron experiments

For nldm low iron Iron (II) sulfate heptahydrate across organisms