Experiment set14S235 for Enterobacter sp. TBS_079

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RCH2_defined_glucose plate, 1:1 coculture with Streptomyces_sp._SAI_041

Group: mixed community
Media: RCH2_defined_glucose + RCH2_defined_glucose plate, 1:1 coculture with Streptomyces_sp._SAI_041
Culturing: Enterobacter_TBS_079_ML3a, agar plate, Aerobic, at 30 (C), (Solid)
By: Robin on 4/17/25
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 20 mM D-Glucose, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

Specific Phenotypes

For 7 genes in this experiment

For mixed community RCH2_defined_glucose plate, 1:1 coculture with Streptomyces_sp._SAI_041 in Enterobacter sp. TBS_079

For mixed community RCH2_defined_glucose plate, 1:1 coculture with Streptomyces_sp._SAI_041 across organisms

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
demethylmenaquinol-4 biosynthesis 1 1 1
demethylmenaquinol-6 biosynthesis I 1 1 1
demethylmenaquinol-9 biosynthesis 1 1 1
demethylmenaquinol-8 biosynthesis I 1 1 1
menaquinol-11 biosynthesis 2 2 1
menaquinol-12 biosynthesis 2 2 1
menaquinol-13 biosynthesis 2 2 1
menaquinol-10 biosynthesis 2 2 1
menaquinol-7 biosynthesis 2 2 1
superpathway of demethylmenaquinol-8 biosynthesis I 9 9 1
superpathway of demethylmenaquinol-6 biosynthesis I 9 8 1
superpathway of demethylmenaquinol-9 biosynthesis 9 8 1
superpathway of menaquinol-8 biosynthesis I 10 10 1
superpathway of menaquinol-7 biosynthesis 10 9 1
superpathway of menaquinol-11 biosynthesis 10 9 1
superpathway of menaquinol-12 biosynthesis 10 9 1
superpathway of menaquinol-10 biosynthesis 10 9 1
superpathway of menaquinol-9 biosynthesis 10 9 1
superpathway of menaquinol-13 biosynthesis 10 9 1
superpathway of menaquinol-6 biosynthesis 10 9 1
superpathway of chorismate metabolism 59 54 1