Experiment set14S191 for Phocaeicola vulgatus CL09T03C04

Sodium pyrophosphate dibasic 1 mM phosphorus source

Group: phosphorus source
Media: Varel_Bryant_medium_Glucose_minusP + Sodium pyrophosphate dibasic (1 mM)
Culturing: Bvulgatus_CL09T03C04_ML5, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya on 7/3/25
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, 20 mM D-Glucose, Mineral 3B solution minus phosphorus (6.6 mM Potassium Chloride, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 8 genes in this experiment

For phosphorus source Sodium pyrophosphate dibasic in Phocaeicola vulgatus CL09T03C04

For phosphorus source Sodium pyrophosphate dibasic across organisms

SEED Subsystems

Subsystem	#Specific
Protein degradation	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.