Experiment set14S149 for Phocaeicola vulgatus CL09T03C04

FSL 10 mg/mL carbon source

Group: carbon source
Media: Varel_Bryant_medium + FSL (10 mg/mL)
Culturing: Bvulgatus_CL09T03C04_ML5, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya on 7/3/25
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 4 genes in this experiment

For carbon source FSL in Phocaeicola vulgatus CL09T03C04

For carbon source FSL across organisms

SEED Subsystems

Subsystem	#Specific
Galactosylceramide and Sulfatide metabolism	1
Lactose and Galactose Uptake and Utilization	1
Lactose utilization	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Other glycan degradation
• Galactose metabolism
• Glycosaminoglycan degradation
• Sphingolipid metabolism
• Glycosphingolipid biosynthesis - ganglio series

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
lactose degradation III	1	1	1
xyloglucan degradation II (exoglucanase)	8	5	5