Experiment set14S106 for Phocaeicola vulgatus CL09T03C04
Sucrose 10 mM carbon source
Group: carbon sourceMedia: Varel_Bryant_medium + Sucrose (10 mM)
Culturing: Bvulgatus_CL09T03C04_ML5, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya on 6/18/25
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)
Specific Phenotypes
For 2 genes in this experiment
For carbon source Sucrose in Phocaeicola vulgatus CL09T03C04
For carbon source Sucrose across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Folate Biosynthesis | 1 |
| One-carbon metabolism by tetrahydropterines | 1 |
| Potassium homeostasis | 1 |
| Serine-glyoxylate cycle | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| folate transformations III (E. coli) | 9 | 8 | 1 |
| folate transformations II (plants) | 11 | 9 | 1 |
| folate transformations I | 13 | 9 | 1 |