Experiment set14IT088 for Pseudomonas fluorescens SBW25-INTG
Glucose (C) and L-Glutamine (N); with TAPS; with Sodium chloride
Group: stressMedia: MTM_noNitrogen_noCarbon + D-Glucose (10 mM) + L-Glutamine (10 mM) + Sodium Chloride (400 Mm), pH=8.5
Culturing: PseudoSBW25_INTG_ML3, 96 deep-well microplate; 1.2 mL volume, Aerobic, at 33 (C), shaken=1200 rpm
By: Joshua Elmore on September 1, 2021
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM TAPS sodium salt, 4.3 mM Sodium Chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)
Specific Phenotypes
For 8 genes in this experiment
For stress D-Glucose in Pseudomonas fluorescens SBW25-INTG
For stress D-Glucose across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Calvin-Benson cycle | 1 |
| Pentose phosphate pathway | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Pentose phosphate pathway
- Carbon fixation in photosynthetic organisms
- Biosynthesis of ansamycins
- Biosynthesis of phenylpropanoids
- Biosynthesis of alkaloids derived from shikimate pathway
- Biosynthesis of plant hormones
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| pentose phosphate pathway (partial) | 3 | 3 | 2 |
| pentose phosphate pathway (non-oxidative branch) I | 5 | 5 | 2 |
| pentose phosphate pathway (non-oxidative branch) II | 6 | 5 | 2 |
| D-apiose degradation I | 3 | 2 | 1 |
| pentose phosphate pathway | 8 | 8 | 2 |
| formaldehyde assimilation II (assimilatory RuMP Cycle) | 9 | 6 | 2 |
| Rubisco shunt | 10 | 8 | 2 |
| formaldehyde assimilation III (dihydroxyacetone cycle) | 12 | 10 | 2 |
| Calvin-Benson-Bassham cycle | 13 | 10 | 2 |
| superpathway of glucose and xylose degradation | 17 | 16 | 2 |
| oxygenic photosynthesis | 17 | 11 | 2 |
| ethene biosynthesis V (engineered) | 25 | 18 | 2 |
| photosynthetic 3-hydroxybutanoate biosynthesis (engineered) | 26 | 19 | 2 |
| 1-butanol autotrophic biosynthesis (engineered) | 27 | 19 | 2 |
| Bifidobacterium shunt | 15 | 12 | 1 |