Experiment set14IT077 for Pseudomonas fluorescens FW300-N2E2

Compare to:

10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine dihydrochloride

200 most detrimental genes:

  gene name fitness t score description  
Pf6N2E2_5346 +2.1 2.9 rRNA small subunit methyltransferase I compare
Pf6N2E2_5936 +2.0 2.1 Low molecular weight protein tyrosine phosphatase (EC 3.1.3.48) compare
Pf6N2E2_623 +1.9 1.1 FIG00954674: hypothetical protein compare
Pf6N2E2_2508 +1.8 7.3 hypothetical protein compare
Pf6N2E2_2518 +1.8 2.1 Cyclohexadienyl dehydrogenase (EC 1.3.1.12)(EC 1.3.1.43) / 5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19) compare
Pf6N2E2_2152 +1.6 2.9 hypothetical protein compare
Pf6N2E2_2597 +1.5 12.1 Flagellar regulatory protein FleQ compare
Pf6N2E2_2578 +1.5 5.0 Flagellar biosynthesis protein FliR compare
Pf6N2E2_180 +1.4 2.6 Transcriptional regulator, MerR family compare
Pf6N2E2_5785 +1.4 2.6 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf6N2E2_2507 +1.4 8.2 Undecaprenyl-phosphate N-acetylglucosaminyl 1-phosphate transferase (EC 2.7.8.-) compare
Pf6N2E2_2790 +1.4 2.9 Helix-turn-helix motif compare
Pf6N2E2_1169 +1.4 2.1 TnpR protein compare
Pf6N2E2_3074 +1.3 1.1 FIG00960973: hypothetical protein compare
Pf6N2E2_2536 +1.3 1.4 Fumarate hydratase class II (EC 4.2.1.2) compare
Pf6N2E2_4797 +1.3 0.8 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase (EC 2.7.6.3) compare
Pf6N2E2_2979 +1.3 5.2 Histone acetyltransferase HPA2 and related acetyltransferases conserved
Pf6N2E2_3696 +1.3 0.9 hypothetical protein compare
Pf6N2E2_4418 +1.3 1.1 Diaminopimelate epimerase (EC 5.1.1.7) compare
Pf6N2E2_2580 +1.3 4.0 Flagellar biosynthesis protein FliP compare
Pf6N2E2_5150 +1.2 7.0 Glucose-6-phosphate isomerase (EC 5.3.1.9) compare
Pf6N2E2_4810 +1.2 7.0 Glucose-1-phosphate thymidylyltransferase (EC 2.7.7.24) compare
Pf6N2E2_2592 +1.2 6.7 Flagellar motor switch protein FliG compare
Pf6N2E2_282 +1.2 0.8 GNAT family acetyltransferase YjcF compare
Pf6N2E2_5695 +1.2 8.2 Flagellar basal-body rod protein FlgC compare
Pf6N2E2_2583 +1.2 7.3 Flagellar motor switch protein FliM compare
Pf6N2E2_2594 +1.2 8.6 Flagellar hook-basal body complex protein FliE compare
Pf6N2E2_1248 +1.2 1.4 MutT/nudix family protein compare
Pf6N2E2_2591 +1.2 5.9 Flagellar assembly protein FliH compare
Pf6N2E2_672 +1.2 1.8 ABC-type polar amino acid transport system, ATPase component compare
Pf6N2E2_2593 +1.1 6.9 Flagellar M-ring protein FliF compare
Pf6N2E2_5405 +1.1 5.6 ABC transporter for D-Alanine, ATPase component (from data) compare
Pf6N2E2_3761 +1.1 8.5 Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-) compare
Pf6N2E2_2590 +1.1 8.2 Flagellum-specific ATP synthase FliI compare
Pf6N2E2_6035 +1.1 2.4 alpha/beta hydrolase fold compare
Pf6N2E2_4710 +1.1 2.6 hypothetical protein compare
Pf6N2E2_613 +1.1 1.2 lipoprotein, putative compare
Pf6N2E2_659 +1.1 1.6 Transcriptional regulatory protein ompR compare
Pf6N2E2_5567 +1.1 1.9 Glutamate Aspartate transport ATP-binding protein GltL (TC 3.A.1.3.4) compare
Pf6N2E2_2628 +1.1 4.9 Flagellar basal-body rod protein FlgF compare
Pf6N2E2_5404 +1.1 7.1 ABC transporter for D-Alanine, permease component 1 (from data) compare
Pf6N2E2_338 +1.1 1.3 Transcriptional regulator, AraC family compare
Pf6N2E2_2577 +1.1 7.3 Flagellar biosynthesis protein FlhB compare
Pf6N2E2_5403 +1.1 7.5 ABC transporter for D-Alanine, permease component 2 (from data) compare
Pf6N2E2_1834 +1.1 1.8 Enoyl-CoA hydratase (EC 4.2.1.17) compare
Pf6N2E2_1470 +1.1 2.9 Transcriptional regulator, LysR family compare
Pf6N2E2_2573 +1.1 6.9 RNA polymerase sigma factor for flagellar operon compare
Pf6N2E2_3373 +1.0 2.3 Outer membrane lipoprotein SmpA, a component of the essential YaeT outer-membrane protein assembly complex compare
Pf6N2E2_2896 +1.0 2.8 DNA-binding response regulator GltR, controls specific porins for the entry of glucose compare
Pf6N2E2_367 +1.0 1.9 Acetylornithine deacetylase (EC 3.5.1.16) compare
Pf6N2E2_546 +1.0 1.3 FIG00966904: hypothetical protein compare
Pf6N2E2_5677 +1.0 0.5 Carbon storage regulator compare
Pf6N2E2_5694 +1.0 5.3 Flagellar basal-body rod protein FlgB compare
Pf6N2E2_5697 +1.0 8.5 Flagellar hook protein FlgE compare
Pf6N2E2_5689 +1.0 3.9 Flagellar biosynthesis protein FlgN compare
Pf6N2E2_698 +1.0 1.9 Heme d1 biosynthesis protein NirH compare
Pf6N2E2_2576 +1.0 7.8 Flagellar biosynthesis protein FlhA compare
Pf6N2E2_1743 +1.0 1.9 Putrescine transport ATP-binding protein PotA (TC 3.A.1.11.1) compare
Pf6N2E2_5671 +1.0 5.5 Succinylglutamate desuccinylase (EC 3.5.1.96) compare
Pf6N2E2_1339 +1.0 1.9 Acetylornithine deacetylase (EC 3.5.1.16) compare
Pf6N2E2_88 +1.0 1.3 hypothetical protein compare
Pf6N2E2_349 +1.0 2.1 hypothetical protein compare
Pf6N2E2_2579 +1.0 5.2 Flagellar biosynthesis protein FliQ compare
Pf6N2E2_3624 +1.0 6.2 Flagellar motor rotation protein MotB compare
Pf6N2E2_1336 +0.9 1.9 AraC family transcription regulator compare
Pf6N2E2_4276 +0.9 4.3 Phosphogluconate repressor HexR, RpiR family compare
Pf6N2E2_6164 +0.9 0.7 tRNA-Val-TAC compare
Pf6N2E2_5494 +0.9 5.9 Pyruvate kinase (EC 2.7.1.40) compare
Pf6N2E2_919 +0.9 1.1 FIG00954373: hypothetical protein compare
Pf6N2E2_2585 +0.9 6.9 Flagellar hook-length control protein FliK compare
Pf6N2E2_2397 +0.9 1.9 Two-component system response regulator QseB compare
Pf6N2E2_5841 +0.9 0.7 GlcG protein compare
Pf6N2E2_1213 +0.9 2.4 hypothetical protein compare
Pf6N2E2_2602 +0.9 6.4 Flagellin protein FlaA compare
Pf6N2E2_2699 +0.9 5.8 Fe(2+)/alpha-ketoglutarate-dependent dioxygenase LpxO compare
Pf6N2E2_5758 +0.9 1.6 FIG00957314: hypothetical protein compare
Pf6N2E2_5774 +0.9 3.3 FIG00954549: hypothetical protein compare
Pf6N2E2_2563 +0.9 7.6 FIG00953562: hypothetical protein compare
Pf6N2E2_5640 +0.9 1.8 FIG00955597: hypothetical protein compare
Pf6N2E2_2626 +0.9 6.2 Flagellar L-ring protein FlgH compare
Pf6N2E2_1027 +0.9 1.6 Cysteine dioxygenase (EC 1.13.11.20) compare
Pf6N2E2_5402 +0.9 5.3 ABC transporter for D-Alanine, periplasmic substrate-binding component (from data) compare
Pf6N2E2_5705 +0.9 1.4 Sensor protein PhoQ (EC 2.7.13.3) compare
Pf6N2E2_4300 +0.9 1.9 Uncharacterized protein similar to VCA0109 compare
Pf6N2E2_460 +0.9 1.3 Glycerate kinase (EC 2.7.1.31) compare
Pf6N2E2_1656 +0.9 1.2 hypothetical protein compare
Pf6N2E2_3268 +0.9 6.1 Ribosome hibernation protein YhbH compare
Pf6N2E2_5534 +0.9 1.2 FIG000859: hypothetical protein YebC compare
Pf6N2E2_2622 +0.8 6.4 Flagellar hook-associated protein FlgL compare
Pf6N2E2_1032 +0.8 1.3 Alkanesulfonates ABC transporter ATP-binding protein / Sulfonate ABC transporter, ATP-binding subunit SsuB compare
Pf6N2E2_4982 +0.8 2.2 UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase (EC 6.3.2.-) compare
Pf6N2E2_2589 +0.8 3.8 Flagellar protein FliJ compare
Pf6N2E2_2572 +0.8 1.8 Chemotaxis regulator - transmits chemoreceptor signals to flagelllar motor components CheY compare
Pf6N2E2_288 +0.8 1.2 Isocitrate dehydrogenase [NADP] (EC 1.1.1.42); Monomeric isocitrate dehydrogenase [NADP] (EC 1.1.1.42) compare
Pf6N2E2_955 +0.8 1.7 FIG00953508: hypothetical protein compare
Pf6N2E2_1176 +0.8 2.0 Mll2356 protein compare
Pf6N2E2_2121 +0.8 1.9 hypothetical protein compare
Pf6N2E2_3060 +0.8 1.9 Outer membrane protein H precursor compare
Pf6N2E2_5696 +0.8 4.4 Flagellar basal-body rod modification protein FlgD compare
Pf6N2E2_3569 +0.8 1.5 hypothetical protein compare
Pf6N2E2_2627 +0.8 4.7 Flagellar basal-body rod protein FlgG compare
Pf6N2E2_2526 +0.8 4.3 Similar to phosphoglycolate phosphatase, clustered with ubiquinone biosynthesis SAM-dependent O-methyltransferase compare
Pf6N2E2_1839 +0.8 1.7 2-hydroxycyclohexanecarboxyl-CoA dehydrogenase (EC 1.1.1.-) compare
Pf6N2E2_5771 +0.8 5.6 FKBP-type peptidyl-prolyl cis-trans isomerase FklB (EC 5.2.1.8) compare
Pf6N2E2_2178 +0.8 4.1 FIG00960144: hypothetical protein compare
Pf6N2E2_330 +0.8 2.4 Mesenchymal stem cell protein DSCD75 compare
Pf6N2E2_5392 +0.8 1.9 Molybdenum cofactor biosynthesis protein MoaC compare
Pf6N2E2_1312 +0.8 1.6 4-hydroxyphenylacetate symporter, major facilitator superfamily (MFS) compare
Pf6N2E2_2623 +0.8 6.1 Flagellar hook-associated protein FlgK compare
Pf6N2E2_2582 +0.8 2.9 Flagellar motor switch protein FliN compare
Pf6N2E2_178 +0.8 0.4 Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20) compare
Pf6N2E2_2247 +0.8 1.7 Transcriptional regulator, AraC family compare
Pf6N2E2_2370 +0.8 2.8 Type III secretion inner membrane channel protein (LcrD,HrcV,EscV,SsaV) compare
Pf6N2E2_101 +0.8 2.3 CigR (Putative inner membrane protein) compare
Pf6N2E2_2063 +0.8 1.4 Predicted cobalt transporter CbtA compare
Pf6N2E2_5691 +0.8 5.2 Flagellar basal-body P-ring formation protein FlgA compare
Pf6N2E2_445 +0.8 6.2 UTP--glucose-1-phosphate uridylyltransferase (EC 2.7.7.9) compare
Pf6N2E2_3298 +0.8 2.8 2-ketoglutaric semialdehyde dehydrogenase (EC 1.2.1.26) compare
Pf6N2E2_4263 +0.8 2.2 Putative metal chaperone, involved in Zn homeostasis, GTPase of COG0523 family compare
Pf6N2E2_2599 +0.7 4.2 Flagellar biosynthesis protein FliS compare
Pf6N2E2_99 +0.7 2.3 hypothetical protein compare
Pf6N2E2_2724 +0.7 2.2 Nitroreductase family protein compare
Pf6N2E2_4435 +0.7 4.9 Regulator of sigma D conserved
Pf6N2E2_942 +0.7 1.0 Mll6465 protein compare
Pf6N2E2_726 +0.7 2.0 Nitrate/nitrite sensor protein (EC 2.7.3.-) compare
Pf6N2E2_6069 +0.7 2.7 FIG049111: Hypothetical protein in pyoverdin gene cluster compare
Pf6N2E2_443 +0.7 1.8 Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1) compare
Pf6N2E2_2794 +0.7 1.1 putative membrane protein compare
Pf6N2E2_3144 +0.7 5.8 Thiol:disulfide interchange protein DsbC compare
Pf6N2E2_3134 +0.7 0.5 hypothetical protein compare
Pf6N2E2_2624 +0.7 4.6 Flagellar protein FlgJ [peptidoglycan hydrolase] (EC 3.2.1.-) compare
Pf6N2E2_4415 +0.7 1.3 Putative FMN hydrolase (EC 3.1.3.-); 5-Amino-6-(5'-phosphoribitylamino)uracil phosphatase compare
Pf6N2E2_4331 +0.7 1.6 hypothetical protein compare
Pf6N2E2_358 +0.7 1.5 Haloacid dehalogenase, type II compare
Pf6N2E2_2296 +0.7 1.7 FIG00954767: hypothetical protein compare
Pf6N2E2_1987 +0.7 1.3 hypothetical protein compare
Pf6N2E2_2281 +0.7 1.5 Beta N-acetyl-glucosaminidase (EC 3.2.1.52) compare
Pf6N2E2_476 +0.7 1.6 Permease of the drug/metabolite transporter (DMT) superfamily compare
Pf6N2E2_2871 +0.7 1.2 FIG00954271: hypothetical protein compare
Pf6N2E2_2625 +0.7 3.3 Flagellar P-ring protein FlgI compare
Pf6N2E2_234 +0.7 1.2 FIG00454312: hypothetical protein compare
Pf6N2E2_2823 +0.7 2.5 Multidrug efflux RND membrane fusion protein MexC compare
Pf6N2E2_2857 +0.7 1.9 Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1) compare
Pf6N2E2_2175 +0.7 3.3 Transcriptional regulator, RpiR family compare
Pf6N2E2_6007 +0.7 2.1 Glutathione S-transferase (EC 2.5.1.18) compare
Pf6N2E2_2406 +0.7 3.9 Periplasmic binding protein compare
Pf6N2E2_5396 +0.7 4.5 YheO-like PAS domain compare
Pf6N2E2_3702 +0.7 2.1 FIG00953907: hypothetical protein compare
Pf6N2E2_2605 +0.7 6.5 3-oxoacyl-[acyl-carrier-protein] synthase, KASIII (EC 2.3.1.180) compare
Pf6N2E2_1935 +0.7 1.4 Enoyl-CoA hydratase (EC 4.2.1.17) compare
Pf6N2E2_1162 +0.7 0.7 Transmembrane amino acid efflux protein compare
Pf6N2E2_4181 +0.7 0.6 Organic hydroperoxide resistance protein compare
Pf6N2E2_5664 +0.7 2.7 Arginine pathway regulatory protein ArgR, repressor of arg regulon compare
Pf6N2E2_3982 +0.7 1.4 Tryptophan synthase beta chain like (EC 4.2.1.20) compare
Pf6N2E2_868 +0.7 1.4 Transcriptional regulator, HxlR family compare
Pf6N2E2_663 +0.6 1.5 Transcriptional activator of acetoin dehydrogenase operon AcoR compare
Pf6N2E2_4394 +0.6 1.3 Putrescine utilization regulator compare
Pf6N2E2_579 +0.6 0.6 FIG00962753: hypothetical protein compare
Pf6N2E2_1807 +0.6 2.5 hypothetical protein compare
Pf6N2E2_4479 +0.6 0.9 FIG001590: Putative conserved exported protein precursor compare
Pf6N2E2_1022 +0.6 1.3 Butyryl-CoA dehydrogenase (EC 1.3.99.2) compare
Pf6N2E2_3037 +0.6 3.4 RNA polymerase sigma factor RpoS compare
Pf6N2E2_4541 +0.6 0.8 hypothetical protein compare
Pf6N2E2_4422 +0.6 1.4 Frataxin homolog CyaY, facilitates iron supply for heme A synthesis or Fe-S cluster assembly compare
Pf6N2E2_4362 +0.6 0.8 DNA-directed RNA polymerase omega subunit (EC 2.7.7.6) compare
Pf6N2E2_4254 +0.6 2.6 HflK protein compare
Pf6N2E2_1552 +0.6 2.1 Transcriptional regulator, LysR family compare
Pf6N2E2_28 +0.6 1.3 4-carboxymuconolactone decarboxylase domain/alkylhydroperoxidase AhpD family core domain protein compare
Pf6N2E2_2693 +0.6 1.6 3-oxoacyl-[acyl-carrier-protein] synthase, KASIII (EC 2.3.1.180) compare
Pf6N2E2_21 +0.6 1.6 Sigma-70 factor FpvI (ECF subfamily), controling pyoverdin biosynthesis compare
Pf6N2E2_3763 +0.6 0.9 hypothetical protein compare
Pf6N2E2_3625 +0.6 4.7 Flagellar motor rotation protein MotA compare
Pf6N2E2_3753 +0.6 5.2 DamX, an inner membrane protein involved in bile resistance compare
Pf6N2E2_2584 +0.6 3.4 Flagellar biosynthesis protein FliL compare
Pf6N2E2_649 +0.6 1.2 General secretion pathway protein L compare
Pf6N2E2_4581 +0.6 3.1 FIG015547: peptidase, M16 family compare
Pf6N2E2_4969 +0.6 1.1 hypothetical protein compare
Pf6N2E2_1844 +0.6 1.7 MaoC-related acyl dehydratase compare
Pf6N2E2_5073 +0.6 0.6 Large-conductance mechanosensitive channel compare
Pf6N2E2_309 +0.6 0.9 FIG00957702: hypothetical protein compare
Pf6N2E2_69 +0.6 4.2 Probable type IV pilus assembly FimV-related transmembrane protein compare
Pf6N2E2_2600 +0.6 4.7 Flagellar hook-associated protein FliD compare
Pf6N2E2_237 +0.6 1.4 hypothetical protein compare
Pf6N2E2_612 +0.6 1.9 Ketoglutarate semialdehyde dehydrogenase (EC 1.2.1.26) (from data) compare
Pf6N2E2_6130 +0.6 1.5 5S RNA compare
Pf6N2E2_667 +0.6 1.6 Dihydrolipoamide acetyltransferase component (E2) of acetoin dehydrogenase complex (EC 2.3.1.-) compare
Pf6N2E2_3871 +0.6 4.3 FIG00553872: hypothetical protein compare
Pf6N2E2_861 +0.6 1.8 Transcriptional regulator, LysR family compare
Pf6N2E2_103 +0.6 2.1 Various polyols ABC transporter, ATP-binding component compare
Pf6N2E2_4072 +0.6 1.4 Phosphate transport ATP-binding protein PstB (TC 3.A.1.7.1) compare
Pf6N2E2_2402 +0.6 0.9 hypothetical protein compare
Pf6N2E2_5987 +0.6 2.2 OmpA family protein compare
Pf6N2E2_2075 +0.6 2.3 Peptidase, U7 family compare
Pf6N2E2_4928 +0.6 0.5 Permeases of the major facilitator superfamily compare
Pf6N2E2_2267 +0.6 1.5 Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-) compare
Pf6N2E2_1808 +0.6 2.1 Transcriptional regulator compare
Pf6N2E2_3204 +0.6 1.4 Rho-specific inhibitor of transcription termination (YaeO) compare
Pf6N2E2_4162 +0.6 0.7 DNA polymerase I (EC 2.7.7.7) compare
Pf6N2E2_2256 +0.6 2.0 hypothetical protein compare
Pf6N2E2_728 +0.6 1.5 Nitrate/nitrite transporter compare


Specific Phenotypes

For 10 genes in this experiment

For resistance P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine dihydrochloride in Pseudomonas fluorescens FW300-N2E2

For resistance P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine dihydrochloride across organisms