Experiment set14IT077 for Pseudomonas fluorescens FW300-N2E2

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10% P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine dihydrochloride

Group: resistance
Media: RCH2_defined_putrescine_20mM + P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine dihydrochloride (10 percent), pH=7
Culturing: pseudo6_N2E2_ML5c, 15 mL tube, aerobic, at 30 (C), shaken=225 rpm
By: Lauren_Andrew on 12/2/2016

Specific Phenotypes

For 10 genes in this experiment

For resistance P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine dihydrochloride in Pseudomonas fluorescens FW300-N2E2

For resistance P. fluorescens FW300-N2C3 spent media from growth in 20 mM putrescine dihydrochloride across organisms

SEED Subsystems

Subsystem #Specific
Polyamine Metabolism 2
Branched-Chain Amino Acid Biosynthesis 1
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis 1
Pyruvate Alanine Serine Interconversions 1
Queuosine-Archaeosine Biosynthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-glutamine biosynthesis I 1 1 1
putrescine degradation II 4 4 2
ammonia assimilation cycle II 2 2 1
β-alanine degradation II 2 2 1
4-aminobutanoate degradation II 2 2 1
putrescine degradation V 2 2 1
ammonia assimilation cycle I 2 2 1
4-aminobutanoate degradation III 2 2 1
4-aminobutanoate degradation I 2 1 1
superpathway of ammonia assimilation (plants) 3 3 1
ammonia assimilation cycle III 3 3 1
L-aspartate degradation III (anaerobic) 3 2 1
superpathway of 4-aminobutanoate degradation 3 2 1
L-aspartate degradation II (aerobic) 3 2 1
superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation 11 9 3
superpathway of ornithine degradation 8 7 2
GABA shunt II 4 2 1
GABA shunt I 4 1 1
superpathway of L-arginine and L-ornithine degradation 13 11 3
β-alanine biosynthesis II 6 5 1
L-glutamate and L-glutamine biosynthesis 7 6 1
4-aminobutanoate degradation V 7 2 1
L-arginine biosynthesis II (acetyl cycle) 10 10 1
superpathway of coenzyme A biosynthesis II (plants) 10 9 1
nicotine degradation I (pyridine pathway) 17 5 1