Experiment set14IT069 for Klebsiella michiganensis M5al

LB 0.5x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL

Group: mixed community
Media: LB (0.5x) + Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL
Culturing: Koxy_ML2a, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Trenton on 7/18/2018
Media components: 5 g/L Tryptone, 2.5 g/L Yeast Extract, 2.5 g/L Sodium Chloride (final concentrations)
Growth plate: Plate 3 D3

Specific Phenotypes

For 4 genes in this experiment

For mixed community Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL in Klebsiella michiganensis M5al

For mixed community Mixed culture; Trichoderma virens G41 spores at 2.5*10^4 spores/mL across organisms

SEED Subsystems

Subsystem	#Specific
Lysine Biosynthesis DAP Pathway	1
Methionine Biosynthesis	1
Threonine and Homoserine Biosynthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glycine, serine and threonine metabolism
• Lysine biosynthesis
• Biosynthesis of plant hormones
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
adenosine nucleotides degradation III	1	1	1
L-homoserine biosynthesis	3	3	2
superpathway of L-threonine biosynthesis	6	6	2
superpathway of L-homoserine and L-methionine biosynthesis	8	8	2
dipicolinate biosynthesis	4	3	1
spermidine biosynthesis II	4	2	1
L-methionine biosynthesis IV	4	2	1
superpathway of S-adenosyl-L-methionine biosynthesis	9	9	2
superpathway of L-methionine biosynthesis (transsulfuration)	9	9	2
ectoine biosynthesis	5	3	1
superpathway of L-methionine biosynthesis (by sulfhydrylation)	12	10	2
norspermidine biosynthesis	6	4	1
superpathway of L-isoleucine biosynthesis I	13	13	2
L-lysine biosynthesis III	7	6	1
L-lysine biosynthesis VI	7	6	1
3-dehydroquinate biosynthesis II (archaea)	7	3	1
cremeomycin biosynthesis	7	2	1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II	15	13	2
superpathway of polyamine biosynthesis III	8	4	1
grixazone biosynthesis	8	2	1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I	18	18	2
L-lysine biosynthesis I	9	9	1
L-lysine biosynthesis II	9	6	1
peptidoglycan recycling II	10	7	1
chorismate biosynthesis II (archaea)	12	8	1
aspartate superpathway	25	24	2
peptidoglycan recycling I	14	14	1
platensimycin biosynthesis	26	6	1