Experiment set14IT066 for Pseudomonas fluorescens FW300-N2E2

Compare to:

10% P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine dihydrochloride

200 most important genes:

  gene name fitness t score description  
Pf6N2E2_2258 -4.9 -31.1 Transaldolase (EC 2.2.1.2) compare
Pf6N2E2_4509 -4.0 -16.3 Gamma-glutamyl-putrescine synthetase (EC 6.3.1.11) (from data) conserved
Pf6N2E2_4511 -3.7 -16.4 glutamine synthetase family protein compare
Pf6N2E2_71 -3.7 -7.5 Phosphoribosylanthranilate isomerase (EC 5.3.1.24) compare
Pf6N2E2_5156 -3.6 -7.2 Two-component sensor CbrB: intrcellular carbon:nitrogen balance compare
Pf6N2E2_5338 -3.6 -15.7 Ubiquinol--cytochrome c reductase, cytochrome B subunit (EC 1.10.2.2) compare
Pf6N2E2_3782 -3.6 -4.8 Phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19) compare
Pf6N2E2_4826 -3.5 -6.1 Indole-3-glycerol phosphate synthase (EC 4.1.1.48) compare
Pf6N2E2_4825 -3.3 -9.7 Anthranilate phosphoribosyltransferase (EC 2.4.2.18) compare
Pf6N2E2_64 -3.3 -3.6 3-isopropylmalate dehydratase small subunit (EC 4.2.1.33) compare
Pf6N2E2_3610 -3.3 -7.3 ATP phosphoribosyltransferase regulatory subunit (EC 2.4.2.17) compare
Pf6N2E2_4597 -3.2 -8.3 Homoserine O-acetyltransferase (EC 2.3.1.31) compare
Pf6N2E2_3839 -3.2 -7.2 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide isomerase (EC 5.3.1.16) (from data) compare
Pf6N2E2_4515 -3.2 -9.1 Putrescine transport ATP-binding protein PotG (TC 3.A.1.11.2) compare
Pf6N2E2_4187 -3.2 -7.4 Lipid A biosynthesis lauroyl acyltransferase (EC 2.3.1.-) compare
Pf6N2E2_5339 -3.1 -10.3 ubiquinol cytochrome C oxidoreductase, cytochrome C1 subunit compare
Pf6N2E2_4513 -3.1 -11.6 Putrescine ABC transporter putrescine-binding protein PotF (TC 3.A.1.11.2) compare
Pf6N2E2_3842 -3.1 -5.7 Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19) compare
Pf6N2E2_5177 -3.1 -10.6 Ketol-acid reductoisomerase (EC 1.1.1.86) compare
Pf6N2E2_4048 -3.1 -6.1 Tryptophan synthase beta chain (EC 4.2.1.20) compare
Pf6N2E2_3932 -3.1 -6.5 D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95) compare
Pf6N2E2_3253 -2.9 -7.4 ATP phosphoribosyltransferase (EC 2.4.2.17) (from data) compare
Pf6N2E2_3783 -2.9 -6.5 phosphoribosyl-ATP pyrophosphatase (EC 3.6.1.31) (from data) compare
Pf6N2E2_77 -2.9 -4.9 O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) / O-succinylhomoserine sulfhydrylase (EC 2.5.1.48) compare
Pf6N2E2_4516 -2.9 -9.1 Putrescine transport system permease protein PotH (TC 3.A.1.11.2) compare
Pf6N2E2_4512 -2.8 -15.0 Gamma-aminobutyrate:alpha-ketoglutarate aminotransferase (EC 2.6.1.19) (from data) conserved
Pf6N2E2_4391 -2.7 -12.7 D-amino acid dehydrogenase small subunit (EC 1.4.99.1) compare
Pf6N2E2_4383 -2.7 -10.5 gamma-glutamyl-gamma-aminobutyraldehyde dehydrogenase (EC 1.2.1.99); 4-guanidinobutyraldehyde dehydrogenase (EC 1.2.1.54) (from data) compare
Pf6N2E2_4059 -2.6 -1.5 DNA-binding protein HU-alpha compare
Pf6N2E2_5337 -2.6 -10.5 Ubiquinol-cytochrome C reductase iron-sulfur subunit (EC 1.10.2.2) compare
Pf6N2E2_3630 -2.6 -3.0 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf6N2E2_2261 -2.5 -16.6 VacJ-like lipoprotein precursor compare
Pf6N2E2_66 -2.5 -8.7 3-isopropylmalate dehydrogenase (EC 1.1.1.85) compare
Pf6N2E2_80 -2.4 -10.1 gamma-glutamylputrescine oxidase (EC 1.4.3.-) (from data) conserved
Pf6N2E2_3260 -2.4 -12.1 Uncharacterized ABC transporter, ATP-binding protein YrbF compare
Pf6N2E2_4596 -2.4 -6.2 Homoserine O-acetyltransferase (EC 2.3.1.31) compare
Pf6N2E2_2073 -2.3 -6.8 Sulfite reductase [NADPH] hemoprotein beta-component (EC 1.8.1.2) compare
Pf6N2E2_3869 -2.3 -8.8 Branched-chain amino acid transport protein AzlC compare
Pf6N2E2_63 -2.3 -4.1 3-isopropylmalate dehydratase large subunit (EC 4.2.1.33) compare
Pf6N2E2_5157 -2.3 -11.1 Two-component sensor CbrA: intrcellular carbon:nitrogen balance compare
Pf6N2E2_3300 -2.3 -3.0 Predicted D-glucarate or D-galactorate regulator, GntR family compare
Pf6N2E2_4803 -2.3 -2.0 Bis(5'-nucleosyl)-tetraphosphatase, symmetrical (EC 3.6.1.41) compare
Pf6N2E2_3257 -2.2 -9.1 Uncharacterized ABC transporter, auxiliary component YrbC compare
Pf6N2E2_4514 -2.2 -9.2 Putrescine ABC transporter putrescine-binding protein PotF (TC 3.A.1.11.2) conserved
Pf6N2E2_156 -2.2 -1.9 Carbon storage regulator compare
Pf6N2E2_4743 -2.1 -1.2 hypothetical protein compare
Pf6N2E2_4564 -2.1 -8.4 Dihydroxy-acid dehydratase (EC 4.2.1.9) compare
Pf6N2E2_2753 -2.1 -2.9 HtrA protease/chaperone protein compare
Pf6N2E2_3248 -2.0 -5.2 Probable transmembrane protein compare
Pf6N2E2_5894 -2.0 -7.0 Putative threonine efflux protein compare
Pf6N2E2_302 -2.0 -5.7 Siroheme synthase / Precorrin-2 oxidase (EC 1.3.1.76) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4) / Uroporphyrinogen-III methyltransferase (EC 2.1.1.107) compare
Pf6N2E2_3258 -2.0 -9.6 Uncharacterized ABC transporter, periplasmic component YrbD compare
Pf6N2E2_3521 -2.0 -7.1 Sensor histidine kinase/response regulator compare
Pf6N2E2_1450 -1.9 -3.0 FIG00960438: hypothetical protein compare
Pf6N2E2_2552 -1.9 -7.4 Cytochrome c heme lyase subunit CcmH compare
Pf6N2E2_5176 -1.8 -1.8 Acetolactate synthase small subunit (EC 2.2.1.6) compare
Pf6N2E2_2318 -1.8 -10.6 Type cbb3 cytochrome oxidase biogenesis protein CcoI; Copper-translocating P-type ATPase (EC 3.6.3.4) compare
Pf6N2E2_5728 -1.8 -4.2 Phosphoribosylglycinamide formyltransferase (EC 2.1.2.2) compare
Pf6N2E2_4351 -1.7 -9.3 Predicted signal transduction protein compare
Pf6N2E2_3940 -1.7 -8.7 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf6N2E2_5545 -1.7 -2.7 Queuosine Biosynthesis QueE Radical SAM compare
Pf6N2E2_3656 -1.7 -5.0 Probable transcription regulator Mig-14 compare
Pf6N2E2_5899 -1.7 -7.1 Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system compare
Pf6N2E2_3665 -1.6 -1.7 serine/threonine protein kinase compare
Pf6N2E2_3786 -1.6 -4.2 Twin-arginine translocation protein TatC compare
Pf6N2E2_3655 -1.6 -12.7 Hypothetical protein FIG015671 in large core OS assembly cluster compare
Pf6N2E2_5158 -1.6 -6.3 FIG00954153: hypothetical protein compare
Pf6N2E2_3170 -1.6 -7.3 2-isopropylmalate synthase (EC 2.3.3.13) compare
Pf6N2E2_4800 -1.6 -11.1 yeaH component of nitrogen-related signalling system yeaH (of yeaGH-ycgB) (from data) compare
Pf6N2E2_2752 -1.5 -7.9 Exported zinc metalloprotease YfgC precursor compare
Pf6N2E2_5257 -1.5 -9.7 Sulfate adenylyltransferase subunit 2 (EC 2.7.7.4) compare
Pf6N2E2_3252 -1.5 -4.9 Histidinol dehydrogenase (EC 1.1.1.23) compare
Pf6N2E2_2869 -1.5 -1.5 Peptide deformylase (EC 3.5.1.88) compare
Pf6N2E2_5258 -1.5 -8.3 Sulfate adenylyltransferase subunit 1 (EC 2.7.7.4) / Adenylylsulfate kinase (EC 2.7.1.25) compare
Pf6N2E2_4799 -1.5 -10.7 ycgB component of nitrogen-related signalling system (of yeaGH-ycgB) (from data) compare
Pf6N2E2_4390 -1.5 -7.8 Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system compare
Pf6N2E2_3899 -1.5 -4.9 Phosphoenolpyruvate carboxykinase [ATP] (EC 4.1.1.49) compare
Pf6N2E2_5160 -1.4 -0.9 C4-type zinc finger protein, DksA/TraR family compare
Pf6N2E2_3720 -1.4 -2.9 FIG027190: Putative transmembrane protein compare
Pf6N2E2_2835 -1.4 -2.6 Beta-ketoadipyl CoA thiolase (EC 2.3.1.-) compare
Pf6N2E2_2317 -1.4 -4.6 Putative analog of CcoH, COG3198 compare
Pf6N2E2_2707 -1.4 -4.6 Allophanate hydrolase 2 subunit 1 (EC 3.5.1.54) compare
Pf6N2E2_2465 -1.4 -5.0 Cys regulon transcriptional activator CysB compare
Pf6N2E2_2588 -1.4 -2.9 Anti-sigma F factor antagonist (spoIIAA-2); Anti-sigma B factor antagonist RsbV compare
Pf6N2E2_510 -1.4 -6.5 Predicted transcriptional regulator of the myo-inositol catabolic operon compare
Pf6N2E2_4050 -1.4 -2.4 FIG00953718: hypothetical protein compare
Pf6N2E2_2442 -1.4 -7.6 sensor histidine kinase/response regulator compare
Pf6N2E2_5679 -1.4 -3.2 hypothetical protein compare
Pf6N2E2_3039 -1.4 -6.3 Protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77) compare
Pf6N2E2_2501 -1.3 -2.5 hypothetical protein compare
Pf6N2E2_2320 -1.3 -3.9 Heavy-metal-associated domain (N-terminus) and membrane-bounded cytochrome biogenesis cycZ-like domain, possible membrane copper tolerance protein compare
Pf6N2E2_4801 -1.3 -10.1 yeaG component of nitrogen-related signalling system (of yeaGH-ycgB) (from data) compare
Pf6N2E2_1652 -1.3 -2.1 Transcriptional regulator, TetR family compare
Pf6N2E2_3393 -1.3 -5.8 Queuosine Biosynthesis QueC ATPase compare
Pf6N2E2_4517 -1.3 -0.9 Putrescine transport system permease protein PotI (TC 3.A.1.11.2) compare
Pf6N2E2_4159 -1.3 -1.6 Cytochrome c4 compare
Pf6N2E2_291 -1.3 -3.4 ATP-dependent Clp protease adaptor protein ClpS compare
Pf6N2E2_5676 -1.2 -2.0 Aspartokinase (EC 2.7.2.4) compare
Pf6N2E2_1694 -1.2 -1.7 FMN reductase (EC 1.5.1.29) compare
Pf6N2E2_3969 -1.2 -1.1 Membrane-fusion protein compare
Pf6N2E2_2455 -1.2 -4.9 FIG00953957: hypothetical protein compare
Pf6N2E2_3251 -1.2 -7.3 histidinol-phosphate aminotransferase (EC 2.6.1.9) (from data) compare
Pf6N2E2_2927 -1.2 -2.7 Putative signal peptide protein compare
Pf6N2E2_477 -1.2 -4.0 Branched-chain amino acid aminotransferase (EC 2.6.1.42) compare
Pf6N2E2_2675 -1.2 -0.6 ATP-dependent RNA helicase SrmB compare
Pf6N2E2_37 -1.2 -1.5 6-carboxytetrahydropterin synthase (EC 4.1.2.50) @ Queuosine biosynthesis QueD, PTPS-I compare
Pf6N2E2_3349 -1.1 -1.1 Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8) compare
Pf6N2E2_968 -1.1 -2.7 FIG00962395: hypothetical protein compare
Pf6N2E2_4607 -1.1 -2.6 Uracil phosphoribosyltransferase (EC 2.4.2.9) / Pyrimidine operon regulatory protein PyrR compare
Pf6N2E2_3085 -1.1 -1.8 FIG00954614: hypothetical protein compare
Pf6N2E2_384 -1.1 -2.3 Glycine cleavage system transcriptional activator compare
Pf6N2E2_2714 -1.1 -1.2 transcriptional regulator, Crp/Fnr family compare
Pf6N2E2_5749 -1.1 -2.5 hypothetical protein compare
Pf6N2E2_5155 -1.1 -1.7 Poly(A) polymerase (EC 2.7.7.19) compare
Pf6N2E2_987 -1.1 -1.7 hypothetical protein compare
Pf6N2E2_2708 -1.1 -4.3 Lactam utilization protein LamB compare
Pf6N2E2_4451 -1.1 -4.2 FIG139438: lipoprotein B compare
Pf6N2E2_1859 -1.1 -2.4 hypothetical protein compare
Pf6N2E2_4824 -1.1 -3.9 Anthranilate synthase, amidotransferase component (EC 4.1.3.27) @ Para-aminobenzoate synthase, amidotransferase component (EC 2.6.1.85) compare
Pf6N2E2_3807 -1.0 -4.5 Histidine utilization repressor compare
Pf6N2E2_2062 -1.0 -2.1 FIG00955561: hypothetical protein compare
Pf6N2E2_3384 -1.0 -3.6 Predicted L-lactate dehydrogenase, hypothetical protein subunit YkgG compare
Pf6N2E2_2473 -1.0 -1.8 Major outer membrane lipoprotein I compare
Pf6N2E2_3738 -1.0 -2.7 General secretion pathway protein L compare
Pf6N2E2_1534 -1.0 -1.3 Probable transmembrane protein compare
Pf6N2E2_5520 -1.0 -4.7 Sensory box histidine kinase compare
Pf6N2E2_5391 -1.0 -6.2 Predicted ATPase related to phosphate starvation-inducible protein PhoH compare
Pf6N2E2_292 -1.0 -2.6 ATP-dependent Clp protease ATP-binding subunit ClpA compare
Pf6N2E2_4454 -1.0 -3.9 Exopolyphosphatase (EC 3.6.1.11) compare
Pf6N2E2_2865 -1.0 -6.6 Gluconate utilization system Gnt-I transcriptional repressor compare
Pf6N2E2_682 -1.0 -4.0 Predicted regulator PutR for proline utilization, GntR family compare
Pf6N2E2_2706 -1.0 -2.2 Allophanate hydrolase 2 subunit 2 (EC 3.5.1.54) compare
Pf6N2E2_5455 -1.0 -2.2 hypothetical protein compare
Pf6N2E2_831 -1.0 -1.7 Ketoacyl reductase hetN (EC 1.3.1.-) compare
Pf6N2E2_1625 -1.0 -2.6 Ferric reductase (1.6.99.14) compare
Pf6N2E2_2466 -1.0 -5.4 Universal stress protein family conserved
Pf6N2E2_3876 -0.9 -2.8 Agmatine deiminase (EC 3.5.3.12) compare
Pf6N2E2_3397 -0.9 -2.0 Phage protein compare
Pf6N2E2_2447 -0.9 -4.7 Probable protease htpX homolog (EC 3.4.24.-) compare
Pf6N2E2_2586 -0.9 -0.8 Hpt domain protein compare
Pf6N2E2_3708 -0.9 -4.9 predicted biotin regulatory protein BioR (GntR family) compare
Pf6N2E2_4584 -0.9 -1.9 Cell division transporter, ATP-binding protein FtsE (TC 3.A.5.1.1) compare
Pf6N2E2_3938 -0.9 -6.3 Threonine dehydratase biosynthetic (EC 4.3.1.19) compare
Pf6N2E2_4762 -0.9 -0.9 Biotin synthesis protein BioH compare
Pf6N2E2_4999 -0.9 -3.8 Membrane-bound lytic murein transglycosylase B precursor (EC 3.2.1.-) compare
Pf6N2E2_4817 -0.9 -7.0 Spermidine Putrescine ABC transporter permease component PotB (TC 3.A.1.11.1) compare
Pf6N2E2_4761 -0.9 -2.3 8-amino-7-oxononanoate synthase (EC 2.3.1.47) compare
Pf6N2E2_1706 -0.9 -2.1 Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3) compare
Pf6N2E2_5093 -0.9 -6.2 ATP-dependent protease La (EC 3.4.21.53) Type II compare
Pf6N2E2_1774 -0.9 -1.1 DNA-binding response regulator, LuxR family, near polyamine transporter compare
Pf6N2E2_5522 -0.9 -1.8 FIG001943: hypothetical protein YajQ compare
Pf6N2E2_4612 -0.9 -1.5 Glutathione synthetase (EC 6.3.2.3) compare
Pf6N2E2_2080 -0.9 -1.9 hypothetical protein compare
Pf6N2E2_5006 -0.9 -1.0 Muramoyltetrapeptide carboxypeptidase (EC 3.4.17.13) compare
Pf6N2E2_1735 -0.9 -1.1 PhnB protein compare
Pf6N2E2_5012 -0.9 -3.8 FIG00954871: hypothetical protein compare
Pf6N2E2_2587 -0.9 -5.5 Serine phosphatase RsbU, regulator of sigma subunit compare
Pf6N2E2_146 -0.9 -1.0 Transcriptional regulator, MarR family compare
Pf6N2E2_1660 -0.8 -1.6 RND efflux system, membrane fusion protein CmeA compare
Pf6N2E2_946 -0.8 -1.5 Transcriptional regulator, LysR family compare
Pf6N2E2_3533 -0.8 -2.9 Cobalt-precorrin-8x methylmutase (EC 5.4.1.2) compare
Pf6N2E2_4751 -0.8 -1.5 response regulator compare
Pf6N2E2_4804 -0.8 -3.3 ApaG protein compare
Pf6N2E2_114 -0.8 -1.6 Polysaccharide biosynthesis protein compare
Pf6N2E2_1662 -0.8 -3.1 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase. compare
Pf6N2E2_2388 -0.8 -2.2 Esterase/lipase/thioesterase family protein compare
Pf6N2E2_2124 -0.8 -1.2 Mg(2+) transport ATPase protein C compare
Pf6N2E2_2071 -0.8 -1.6 hypothetical protein compare
Pf6N2E2_78 -0.8 -3.3 Oxidoreductase, short chain dehydrogenase/reductase family compare
Pf6N2E2_2013 -0.8 -1.0 hypothetical protein compare
Pf6N2E2_3696 -0.8 -0.4 hypothetical protein compare
Pf6N2E2_5175 -0.8 -2.2 Acetolactate synthase large subunit (EC 2.2.1.6) compare
Pf6N2E2_1716 -0.8 -3.2 Phenylacetate-coenzyme A ligase (EC 6.2.1.30) compare
Pf6N2E2_5956 -0.8 -3.5 FIG004453: protein YceG like compare
Pf6N2E2_5986 -0.8 -1.3 Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1) compare
Pf6N2E2_3293 -0.8 -1.8 4-carboxymuconolactone decarboxylase (EC 4.1.1.44) compare
Pf6N2E2_3785 -0.7 -2.2 Twin-arginine translocation protein TatB compare
Pf6N2E2_4985 -0.7 -2.6 Nucleoside-diphosphate-sugar epimerases compare
Pf6N2E2_5929 -0.7 -2.2 FIG00954461: hypothetical protein compare
Pf6N2E2_4759 -0.7 -2.7 Biotin synthase (EC 2.8.1.6) compare
Pf6N2E2_1696 -0.7 -1.9 Alkanesulfonates-binding protein compare
Pf6N2E2_5103 -0.7 -0.4 Glutamate 5-kinase (EC 2.7.2.11) / RNA-binding C-terminal domain PUA compare
Pf6N2E2_939 -0.7 -1.2 hypothetical protein compare
Pf6N2E2_1065 -0.7 -1.8 Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system compare
Pf6N2E2_3834 -0.7 -2.9 Lipoprotein NlpD compare
Pf6N2E2_5950 -0.7 -1.4 Phosphate:acyl-ACP acyltransferase PlsX compare
Pf6N2E2_5020 -0.7 -1.8 FIG00955339: hypothetical protein compare
Pf6N2E2_4047 -0.7 -0.8 Tryptophan synthase alpha chain (EC 4.2.1.20) compare
Pf6N2E2_4715 -0.7 -2.2 Low-specificity L-threonine aldolase (EC 4.1.2.48) compare
Pf6N2E2_3762 -0.7 -2.7 NADP-dependent malic enzyme (EC 1.1.1.40) compare
Pf6N2E2_238 -0.7 -0.8 hypothetical protein compare
Pf6N2E2_3524 -0.7 -2.4 hypothetical protein compare
Pf6N2E2_1110 -0.7 -1.6 FIG00964414: hypothetical protein compare
Pf6N2E2_1131 -0.7 -2.3 RNA polymerase sigma-70 factor, ECF subfamily compare
Pf6N2E2_2316 -0.7 -4.7 Type cbb3 cytochrome oxidase biogenesis protein CcoG, involved in Cu oxidation compare
Pf6N2E2_3868 -0.7 -3.6 Transcriptional regulator, LysR family compare
Pf6N2E2_1500 -0.7 -2.7 LysR family transcriptional regulator lrhA compare
Pf6N2E2_1056 -0.7 -0.3 Quinone oxidoreductase (EC 1.6.5.5) compare
Pf6N2E2_1321 -0.7 -2.5 MoaF protein precursor compare
Pf6N2E2_895 -0.7 -1.5 Transcriptional regulator, HxlR family compare


Specific Phenotypes

For 10 genes in this experiment

For resistance P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine dihydrochloride in Pseudomonas fluorescens FW300-N2E2

For resistance P. fluorescens FW300-N1B4 spent media from growth in 20 mM putrescine dihydrochloride across organisms