Experiment set14IT052 for Klebsiella michiganensis M5al
Fungal_growth_media_mCAFEs_v1 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Group: mixed communityMedia: Fungal_growth_media_mCAFEs_v1 (0.5x) + Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Culturing: Koxy_ML2a, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Trenton on 7/18/2018
Media components: 10 g/L D-Glucose, 0.1 g/L Magnesium Sulfate Heptahydrate, 0.45 g/L Potassium phosphate dibasic, 0.1 g/L Potassium Chloride, 0.5 g/L Ammonium Nitrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Manganese (II) chloride tetrahydrate, 0.001 g/L Cobalt chloride hexahydrate (final concentrations)
Growth plate: Plate 3 A4
Specific Phenotypes
For 2 genes in this experiment
SEED Subsystems
Subsystem | #Specific |
---|---|
Campylobacter Iron Metabolism | 1 |
Pyridoxin (Vitamin B6) Biosynthesis | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
Pathway | #Steps | #Present | #Specific |
---|---|---|---|
pyridoxal 5'-phosphate biosynthesis I | 7 | 7 | 1 |
superpathway of pyridoxal 5'-phosphate biosynthesis and salvage | 12 | 12 | 1 |