Experiment set14IT047 for Klebsiella michiganensis M5al
Fungal_growth_media_mCAFEs_v1 with Phosphomycin disodium salt 0.01 mg/mL
Group: stressMedia: Fungal_growth_media_mCAFEs_v1 + Phosphomycin disodium salt (0.01 mg/mL)
Culturing: Koxy_ML2a, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Trenton on 7/18/2018
Media components: 20 g/L D-Glucose, 0.2 g/L Magnesium Sulfate Heptahydrate, 0.9 g/L Potassium phosphate dibasic, 0.2 g/L Potassium Chloride, 1 g/L Ammonium Nitrate, 0.002 g/L Iron (II) sulfate heptahydrate, 0.002 g/L Zinc sulfate heptahydrate, 0.002 g/L Manganese (II) chloride tetrahydrate, 0.002 g/L Cobalt chloride hexahydrate
Growth plate: Plate 2 D5
Specific Phenotypes
For 11 genes in this experiment
For stress Phosphomycin disodium salt in Klebsiella michiganensis M5al
For stress Phosphomycin disodium salt across organisms
SEED Subsystems
Subsystem | #Specific |
---|---|
Bacterial Cytoskeleton | 2 |
Bacterial Cell Division | 1 |
KDO2-Lipid A biosynthesis | 1 |
Orphan regulatory proteins | 1 |
Pyridoxin (Vitamin B6) Biosynthesis | 1 |
Serine-glyoxylate cycle | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Ascorbate and aldarate metabolism
- Ubiquinone and menaquinone biosynthesis
- Nucleotide sugars metabolism
- Glycosaminoglycan degradation
- Glyoxylate and dicarboxylate metabolism
- Vitamin B6 metabolism
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
Pathway | #Steps | #Present | #Specific |
---|---|---|---|
glyoxylate cycle | 6 | 6 | 1 |
pyridoxal 5'-phosphate biosynthesis I | 7 | 7 | 1 |
peptidoglycan recycling II | 10 | 7 | 1 |
superpathway of pyridoxal 5'-phosphate biosynthesis and salvage | 12 | 12 | 1 |
superpathway of glyoxylate bypass and TCA | 12 | 11 | 1 |
peptidoglycan recycling I | 14 | 14 | 1 |
superpathway of glyoxylate cycle and fatty acid degradation | 14 | 11 | 1 |
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass | 26 | 25 | 1 |