Experiment set14IT038 for Sinorhizobium meliloti 1021

Beta-Lactose carbon source

Group: carbon source
Media: RCH2_defined_noCarbon + Beta-Lactose (10 mM)
Culturing: Smeli_ML6a, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Adam on 10-Jan-22
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

Specific Phenotypes

For 9 genes in this experiment

For carbon source Beta-Lactose in Sinorhizobium meliloti 1021

For carbon source Beta-Lactose across organisms

SEED Subsystems

Subsystem	#Specific
Alkanesulfonate assimilation	1
Alkanesulfonates Utilization	1
D-galactonate catabolism	1
Galactosylceramide and Sulfatide metabolism	1
Glycerol and Glycerol-3-phosphate Uptake and Utilization	1
Inositol catabolism	1
Lactose and Galactose Uptake and Utilization	1
Lactose utilization	1
Ribitol, Xylitol, Arabitol, Mannitol and Sorbitol utilization	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Galactose metabolism
• Tryptophan metabolism
• Methane metabolism
• Carotenoid biosynthesis - General
• Alkaloid biosynthesis I
• Ubiquinone and menaquinone biosynthesis
• Puromycin biosynthesis
• Histidine metabolism
• Phenylalanine metabolism
• gamma-Hexachlorocyclohexane degradation
• Bisphenol A degradation
• Cyanoamino acid metabolism
• Other glycan degradation
• Nucleotide sugars metabolism
• Glycosaminoglycan degradation
• alpha-Linolenic acid metabolism
• Sphingolipid metabolism
• Glycosphingolipid biosynthesis - ganglio series
• Toluene and xylene degradation
• 1- and 2-Methylnaphthalene degradation
• Naphthalene and anthracene degradation
• 1,4-Dichlorobenzene degradation
• Benzoate degradation via CoA ligation
• Trinitrotoluene degradation
• Styrene degradation
• Porphyrin and chlorophyll metabolism
• Limonene and pinene degradation
• Brassinosteroid biosynthesis
• Phenylpropanoid biosynthesis
• Flavonoid biosynthesis
• Isoflavonoid biosynthesis
• Insect hormone biosynthesis
• Biosynthesis of phenylpropanoids
• Biosynthesis of terpenoids and steroids
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
lactose degradation III	1	1	1
two-component alkanesulfonate monooxygenase	2	1	1
bacterial bioluminescence	8	4	3
xyloglucan degradation II (exoglucanase)	8	3	3
5,6-dimethylbenzimidazole biosynthesis I (aerobic)	3	3	1
D-galactonate degradation	3	3	1
S-(2-succinyl)-L-cysteine degradation	4	3	1
dibenzothiophene desulfurization	5	1	1
L-glucose degradation	7	3	1
adenosylcobalamin biosynthesis II (aerobic)	33	30	1