Experiment set14IT033 for Sinorhizobium meliloti 1021

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Lactitol carbon source

Group: carbon source
Media: RCH2_defined_noCarbon + Lactitol (10 mM)
Culturing: Smeli_ML6a, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Adam on 10-Jan-22
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)

Specific Phenotypes

For 19 genes in this experiment

For carbon source Lactitol in Sinorhizobium meliloti 1021

For carbon source Lactitol across organisms

SEED Subsystems

Subsystem #Specific
Lactose and Galactose Uptake and Utilization 2
ABC transporter oligopeptide (TC 3.A.1.5.1) 1
Chitin and N-acetylglucosamine utilization 1
Galactosylceramide and Sulfatide metabolism 1
Lactose utilization 1
Maltose and Maltodextrin Utilization 1
Mannose Metabolism 1
Propionyl-CoA to Succinyl-CoA Module 1
Ribitol, Xylitol, Arabitol, Mannitol and Sorbitol utilization 1
Serine-glyoxylate cycle 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
lactose degradation III 1 1 1
trehalose degradation VI (periplasmic) 2 2 1
xyloglucan degradation II (exoglucanase) 8 3 3
propanoyl CoA degradation I 3 3 1
conversion of succinate to propanoate 3 1 1
2-oxobutanoate degradation I 4 3 1
D-galactose degradation I (Leloir pathway) 5 3 1
pyruvate fermentation to propanoate I 7 4 1
anaerobic energy metabolism (invertebrates, mitochondrial) 10 7 1
L-glutamate degradation VIII (to propanoate) 11 4 1
(S)-lactate fermentation to propanoate, acetate and hydrogen 13 7 1
3-hydroxypropanoate cycle 13 7 1
crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered) 14 3 1
superpathway of L-methionine salvage and degradation 16 9 1
superpathway of anaerobic energy metabolism (invertebrates) 17 12 1
3-hydroxypropanoate/4-hydroxybutanate cycle 18 10 1
superpathway of the 3-hydroxypropanoate cycle 18 7 1
methylaspartate cycle 19 12 1