Experiment set14IT022 for Pseudomonas fluorescens SBW25-INTG
Glucose (C) and Ammonium chloride (N); with MOPS; with Sodium chloride
Group: stressMedia: MME_noNitrogen_noCarbon + D-Glucose (10 mM) + Ammonium chloride (10 mM) + Sodium Chloride (400 mM), pH=7
Culturing: PseudoSBW25_INTG_ML3, 96 deep-well microplate; 1.2 mL volume, Aerobic, at 33 (C), shaken=1200 rpm
By: Joshua Elmore on September 1, 2021
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)
Specific Phenotypes
For 10 genes in this experiment
For stress D-Glucose in Pseudomonas fluorescens SBW25-INTG
For stress D-Glucose across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| D-gluconate and ketogluconates metabolism | 1 |
| Experimental tye | 1 |
| Heme and Siroheme Biosynthesis | 1 |
| Phosphate metabolism | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Urea cycle and metabolism of amino groups
- Arginine and proline metabolism
- Phenylalanine metabolism
- Tryptophan metabolism
- Cyanoamino acid metabolism
- Benzoate degradation via CoA ligation
- Styrene degradation
- Porphyrin and chlorophyll metabolism
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Biosynthesis of plant hormones
MetaCyc Pathways
Pathways that contain genes with specific phenotypes: