Experiment set14IT021 for Klebsiella michiganensis M5al

Compare to:

Fungal_growth_media_mCAFEs_v1 with Sodium nitrate 20 mM

Group: stress
Media: Fungal_growth_media_mCAFEs_v1 + Sodium nitrate (20 mM)
Culturing: Koxy_ML2a, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Trenton on 7/18/2018
Media components: 20 g/L D-Glucose, 0.2 g/L Magnesium Sulfate Heptahydrate, 0.9 g/L Potassium phosphate dibasic, 0.2 g/L Potassium Chloride, 1 g/L Ammonium Nitrate, 0.002 g/L Iron (II) sulfate heptahydrate, 0.002 g/L Zinc sulfate heptahydrate, 0.002 g/L Manganese (II) chloride tetrahydrate, 0.002 g/L Cobalt chloride hexahydrate
Growth plate: Plate 1 D3

Specific Phenotypes

For 13 genes in this experiment

For stress Sodium nitrate in Klebsiella michiganensis M5al

For stress Sodium nitrate across organisms

SEED Subsystems

Subsystem #Specific
Biotin biosynthesis 3
Coenzyme A Biosynthesis 3
DNA repair, UvrABC system 3
Iron acquisition in Vibrio 1
Lipid A modifications 1
Orphan regulatory proteins 1
Pyridoxin (Vitamin B6) Biosynthesis 1
Transport of Iron 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
biotin biosynthesis from 8-amino-7-oxononanoate I 4 4 4
β-alanine biosynthesis III 1 1 1
biotin biosynthesis from 8-amino-7-oxononanoate II 4 3 3
biotin biosynthesis from 8-amino-7-oxononanoate III 5 3 3
phosphopantothenate biosynthesis I 4 4 2
biotin biosynthesis II 6 4 3
superpathway of coenzyme A biosynthesis I (bacteria) 9 9 3
biotin biosynthesis I 15 15 4
phosphopantothenate biosynthesis III (archaea) 4 2 1
superpathway of coenzyme A biosynthesis II (plants) 10 5 2
pyridoxal 5'-phosphate biosynthesis I 7 7 1
superpathway of pyridoxal 5'-phosphate biosynthesis and salvage 12 12 1