Experiment set14IT015 for Klebsiella michiganensis M5al

Fungal_growth_media_mCAFEs_v1 with Vancomycin Hydrochloride Hydrate 0.01 mg/mL

Group: stress
Media: Fungal_growth_media_mCAFEs_v1 + Vancomycin Hydrochloride Hydrate (0.01 mg/mL)
Culturing: Koxy_ML2a, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Trenton on 7/18/2018
Media components: 20 g/L D-Glucose, 0.2 g/L Magnesium Sulfate Heptahydrate, 0.9 g/L Potassium phosphate dibasic, 0.2 g/L Potassium Chloride, 1 g/L Ammonium Nitrate, 0.002 g/L Iron (II) sulfate heptahydrate, 0.002 g/L Zinc sulfate heptahydrate, 0.002 g/L Manganese (II) chloride tetrahydrate, 0.002 g/L Cobalt chloride hexahydrate
Growth plate: Plate 1 C3

Specific Phenotypes

For 5 genes in this experiment

For stress Vancomycin Hydrochloride Hydrate in Klebsiella michiganensis M5al

For stress Vancomycin Hydrochloride Hydrate across organisms

SEED Subsystems

Subsystem	#Specific
Campylobacter Iron Metabolism	1
Copper homeostasis: copper tolerance	1
Phosphate metabolism	1
Pyridoxin (Vitamin B6) Biosynthesis	1
ZZ gjo need homes	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Vitamin B6 metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
pyridoxal 5'-phosphate biosynthesis I	7	7	1
superpathway of pyridoxal 5'-phosphate biosynthesis and salvage	12	12	1