Experiment set14IT008 for Klebsiella michiganensis M5al

Compare to:

Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml

Group: supernatant
Media: Fungal_growth_media_mCAFEs_v1 + supernatant from Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted (0.8 x) + biotin (0.32 ug/ml)
Culturing: Koxy_ML2a, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Trenton on 7/18/2018
Media components: 20 g/L D-Glucose, 0.2 g/L Magnesium Sulfate Heptahydrate, 0.9 g/L Potassium phosphate dibasic, 0.2 g/L Potassium Chloride, 1 g/L Ammonium Nitrate, 0.002 g/L Iron (II) sulfate heptahydrate, 0.002 g/L Zinc sulfate heptahydrate, 0.002 g/L Manganese (II) chloride tetrahydrate, 0.002 g/L Cobalt chloride hexahydrate
Growth plate: Plate 1 B2

Specific Phenotypes

For 6 genes in this experiment

For supernatant Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted in Klebsiella michiganensis M5al

For supernatant Supernatant; Trichoderma harzianum M10 grown in Fungal_growth_media_mCAFEs_v2, filtered and pH-adjusted across organisms

SEED Subsystems

Subsystem #Specific
Methionine Biosynthesis 1
Potassium homeostasis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
S-adenosyl-L-methionine salvage II 3 3 1
S-adenosyl-L-methionine salvage I 4 4 1
L-methionine biosynthesis III 4 2 1
L-methionine biosynthesis I 5 5 1
seleno-amino acid biosynthesis (plants) 5 3 1
L-methionine biosynthesis II 6 5 1
superpathway of L-homoserine and L-methionine biosynthesis 8 8 1
superpathway of L-methionine biosynthesis (transsulfuration) 9 9 1
superpathway of S-adenosyl-L-methionine biosynthesis 9 9 1
superpathway of L-methionine biosynthesis (by sulfhydrylation) 12 10 1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis II 15 13 1
superpathway of L-lysine, L-threonine and L-methionine biosynthesis I 18 18 1
superpathway of seleno-compound metabolism 19 6 1
aspartate superpathway 25 24 1