Experiment set14IT004 for Klebsiella michiganensis M5al
RCH2_defined_noCarbon with Brachypodium distachyon 27-day exudates 3 x
Group: exudatesMedia: RCH2_defined_noCarbon + Brachypodium distachyon 27-day exudates (3 x)
Culturing: Koxy_ML2a, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Trenton on 7/18/2018
Media components: 0.25 g/L Ammonium chloride, 0.1 g/L Potassium Chloride, 0.6 g/L Sodium phosphate monobasic monohydrate, 30 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.03 g/L Magnesium Sulfate Heptahydrate, 0.015 g/L Nitrilotriacetic acid, 0.01 g/L Sodium Chloride, 0.005 g/L Manganese (II) sulfate monohydrate, 0.001 g/L Cobalt chloride hexahydrate, 0.001 g/L Zinc sulfate heptahydrate, 0.001 g/L Calcium chloride dihydrate, 0.001 g/L Iron (II) sulfate heptahydrate, 0.00025 g/L Nickel (II) chloride hexahydrate, 0.0002 g/L Aluminum potassium sulfate dodecahydrate, 0.0001 g/L Copper (II) sulfate pentahydrate, 0.0001 g/L Boric Acid, 0.0001 g/L Sodium Molybdate Dihydrate, 0.003 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.1 mg/L Pyridoxine HCl, 0.05 mg/L 4-Aminobenzoic acid, 0.05 mg/L Lipoic acid, 0.05 mg/L Nicotinic Acid, 0.05 mg/L Riboflavin, 0.05 mg/L Thiamine HCl, 0.05 mg/L calcium pantothenate, 0.02 mg/L biotin, 0.02 mg/L Folic Acid, 0.001 mg/L Cyanocobalamin)
Growth plate: Plate 1 A4
Specific Phenotypes
For 4 genes in this experiment
For exudates Brachypodium distachyon 27-day exudates in Klebsiella michiganensis M5al
For exudates Brachypodium distachyon 27-day exudates across organisms
SEED Subsystems
Subsystem | #Specific |
---|---|
Glycerol and Glycerol-3-phosphate Uptake and Utilization | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
Pathway | #Steps | #Present | #Specific |
---|---|---|---|
glycerophosphodiester degradation | 2 | 2 | 1 |
phosphatidylcholine resynthesis via glycerophosphocholine | 2 | 1 | 1 |
glycerol and glycerophosphodiester degradation | 4 | 4 | 1 |
phospholipid remodeling (phosphatidylethanolamine, yeast) | 4 | 2 | 1 |
anandamide biosynthesis I | 12 | 3 | 1 |