Experiment set14IT002 for Bacteroides thetaiotaomicron VPI-5482

BHIS with Simvastatin 30 uM

Group: stress
Media: BHIS + Simvastatin (30 uM)
Culturing: Btheta_ML6a, 24-well transparent microplate, Anaerobic, at 37 (C), shaken=0 rpm
By: Veronica Escalante on 10/4/19
Media components: 7.7 g/L Calf brains, 9.8 g/L Beef heart, 10 g/L Proteose Peptone, 5 g/L Sodium Chloride, 2.5 g/L Disodium phosphate, 1 g/L L-Cysteine, 2 g/L Sodium bicarbonate, 0.005 g/L Hemin

Specific Phenotypes

For 9 genes in this experiment

For stress Simvastatin in Bacteroides thetaiotaomicron VPI-5482

For stress Simvastatin across organisms

SEED Subsystems

Subsystem	#Specific
Campylobacter Iron Metabolism	1
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Alanine and aspartate metabolism
• Nitrogen metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-glutamate degradation II	2	2	1
L-aspartate degradation II (aerobic)	3	2	1
L-aspartate degradation III (anaerobic)	3	2	1
superpathway of L-aspartate and L-asparagine biosynthesis	4	4	1
N-(1-deoxy-D-fructos-1-yl)-L-asparagine degradation	5	3	1
L-canavanine degradation II	6	1	1