Experiment set14IT001 for Burkholderia phytofirmans PsJN

Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered; 0.5X; growth supplemented with 0.4X LB

Group: supernatant
Media: LB (0.4x) + supernatant from Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered (0.5 X)
Culturing: BFirm_ML3a, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Cat_Manuel_Trenton on 3/3/21
Media components: 4 g/L Tryptone, 2 g/L Yeast Extract, 2 g/L Sodium Chloride (final concentrations)

Specific Phenotypes

For 1 genes in this experiment

For supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered in Burkholderia phytofirmans PsJN

For supernatant Supernatant; Trichoderma atroviride IMI grown in Vogels_fungal_media, filtered across organisms

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Citrate cycle (TCA cycle)
• Glyoxylate and dicarboxylate metabolism
• Propanoate metabolism
• Reductive carboxylate cycle (CO2 fixation)
• Biosynthesis of phenylpropanoids
• Biosynthesis of terpenoids and steroids
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of plant hormones

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
glyoxylate cycle	6	6	2
partial TCA cycle (obligate autotrophs)	8	7	2
nitrogen remobilization from senescing leaves	8	6	2
TCA cycle V (2-oxoglutarate synthase)	9	8	2
TCA cycle IV (2-oxoglutarate decarboxylase)	9	7	2
TCA cycle II (plants and fungi)	9	7	2
TCA cycle VII (acetate-producers)	9	7	2
TCA cycle VI (Helicobacter)	9	6	2
2-methylcitrate cycle I	5	5	1
TCA cycle I (prokaryotic)	10	8	2
TCA cycle III (animals)	10	7	2
reductive TCA cycle I	11	6	2
superpathway of glyoxylate bypass and TCA	12	10	2
2-methylcitrate cycle II	6	5	1
reductive TCA cycle II	12	6	2
superpathway of glyoxylate cycle and fatty acid degradation	14	11	2
mixed acid fermentation	16	13	2
methylaspartate cycle	19	11	2
superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle	22	17	2
ethene biosynthesis V (engineered)	25	18	2
superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass	26	22	2