Experiment set13S62 for Rhodanobacter denitrificans FW104-10B01

R2A_PIPES with Manganese (II) chloride tetrahydrate 1 mM

Group: stress
Media: R2A_PIPES + Manganese (II) chloride tetrahydrate (1 mM), pH=7
Culturing: rhodanobacter_10B01_ML12, 96 well deep well block, Aerobic, at 23 (C), shaken=700 rpm
Growth: about 4.5 generations
By: Hans and Hira on 10-Jan-25
Media components: 0.5 g/L Bacto Peptone, 0.5 g/L casamino acids, 0.5 g/L Yeast Extract, 0.5 g/L D-Glucose, 0.5 g/L Starch, 0.3 g/L Potassium phosphate dibasic, 0.05 g/L Magnesium Sulfate Heptahydrate, 0.3 g/L Sodium pyruvate, 30 mM PIPES sesquisodium salt

Specific Phenotypes

For 6 genes in this experiment

For stress Manganese (II) chloride tetrahydrate in Rhodanobacter denitrificans FW104-10B01

For stress Manganese (II) chloride tetrahydrate across organisms

SEED Subsystems

Subsystem	#Specific
Arginine Biosynthesis extended	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Urea cycle and metabolism of amino groups
• Purine metabolism
• Arginine and proline metabolism
• Lipopolysaccharide biosynthesis
• Folate biosynthesis
• Sulfur metabolism
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
assimilatory sulfate reduction III	3	3	1
assimilatory sulfate reduction I	4	4	1
L-ornithine biosynthesis I	5	5	1
L-arginine biosynthesis III (via N-acetyl-L-citrulline)	9	9	1
L-arginine biosynthesis I (via L-ornithine)	9	8	1
superpathway of sulfate assimilation and cysteine biosynthesis	9	8	1
superpathway of sulfur amino acid biosynthesis (Saccharomyces cerevisiae)	10	8	1
superpathway of L-methionine biosynthesis (by sulfhydrylation)	12	11	1
superpathway of arginine and polyamine biosynthesis	17	12	1