Experiment set13S57 for Rhodanobacter denitrificans FW104-10B01

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R2A_PIPES with Cobalt chloride hexahydrate 256 uM

Group: stress
Media: R2A_PIPES + Cobalt chloride hexahydrate (256 uM), pH=7
Culturing: rhodanobacter_10B01_ML12, 96 well deep well block, Aerobic, at 23 (C), shaken=700 rpm
Growth: about 4.3 generations
By: Hans and Hira on 10-Jan-25
Media components: 0.5 g/L Bacto Peptone, 0.5 g/L casamino acids, 0.5 g/L Yeast Extract, 0.5 g/L D-Glucose, 0.5 g/L Starch, 0.3 g/L Potassium phosphate dibasic, 0.05 g/L Magnesium Sulfate Heptahydrate, 0.3 g/L Sodium pyruvate, 30 mM PIPES sesquisodium salt

Specific Phenotypes

For 7 genes in this experiment

For stress Cobalt chloride hexahydrate in Rhodanobacter denitrificans FW104-10B01

For stress Cobalt chloride hexahydrate across organisms

SEED Subsystems

Subsystem #Specific
Cobalt-zinc-cadmium resistance 1
Cysteine Biosynthesis 1
Glutathione: Biosynthesis and gamma-glutamyl cycle 1
Utilization of glutathione as a sulphur source 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
glutathione degradation (DUG pathway) 2 1 1
peptido-conjugates in tissue regeneration biosynthesis 17 3 3
γ-glutamyl cycle 6 3 1
leukotriene biosynthesis 6 1 1
hypoglycin biosynthesis 14 4 1