Experiment set13S57 for Phocaeicola vulgatus CL09T03C04
Columbia 1x carbon source complex media
Group: carbon source complex mediaMedia: Varel_Bryant_medium + 1x Columbia
Culturing: Bvulgatus_CL09T03C04_ML5, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya on 6/18/25
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)
Columbia 1x includes: 5 g/L Proteose Peptone, 10 g/L Tryptone, 5 g/L Yeast Extract, 3 g/L Beef Heart Infusion, 2.5 g/L D-Glucose, 5 g/L Sodium Chloride, 0.6 g/L Sodium carbonate, 0.02 g/L ammonium iron(II) sulfate hexahydrate, 0.1 g/L Magnesium sulfate, 0.1 g/L L-Cysteine hydrochloride monohydrate, 0.83 g/L Tris(hydroxymethyl)aminomethane, 2.86 g/L Tris hydrochloride, 0.005 g/L Hemin, 0.001 g/L Menadione
Specific Phenotypes
None in this experiment
For Phocaeicola vulgatus CL09T03C04 in carbon source complex media experiments