Experiment set13S50 for Phocaeicola vulgatus CL09T03C04

Sucrose 10 mM carbon source

Group: carbon source
Media: Varel_Bryant_medium + Sucrose (10 mM)
Culturing: Bvulgatus_CL09T03C04_ML5, 96 deep-well microplate; 1.2 mL volume, Anaerobic, at 37 (C), shaken=0 rpm
By: Surya on 6/18/25
Media components: 15 uM Hemin, 134 uM L-Methionine, 15 uM Iron (II) sulfate heptahydrate, 8.25 mM L-Cysteine, 23.8 mM Sodium bicarbonate, Mineral 3B solution (6.6 mM Potassium phosphate monobasic, 15.4 mM Sodium Chloride, 98 uM Magnesium chloride hexahydrate, 176.5 uM Calcium chloride dihydrate, 4.2 uM Cobalt chloride hexahydrate, 50.5 uM Manganese (II) chloride tetrahydrate, 9.3 mM Ammonium chloride, 1.75 mM Sodium sulfate)

Specific Phenotypes

For 3 genes in this experiment

For carbon source Sucrose in Phocaeicola vulgatus CL09T03C04

For carbon source Sucrose across organisms

SEED Subsystems

Subsystem	#Specific
Fatty Acid Biosynthesis FASII	1
Potassium homeostasis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Fatty acid biosynthesis
• 1,1,1-Trichloro-2,2-bis(4-chlorophenyl)ethane (DDT) degradation
• Phenylalanine metabolism
• gamma-Hexachlorocyclohexane degradation
• Fluorobenzoate degradation
• Tryptophan metabolism
• Fluorene degradation
• Propanoate metabolism
• Isoflavonoid biosynthesis
• Biosynthesis of unsaturated fatty acids

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
fatty acid elongation -- saturated	5	5	2
gondoate biosynthesis (anaerobic)	4	4	1
palmitate biosynthesis II (type II fatty acid synthase)	31	29	7
palmitoleate biosynthesis I (from (5Z)-dodec-5-enoate)	9	8	2
oleate biosynthesis IV (anaerobic)	14	13	3
superpathway of fatty acids biosynthesis (E. coli)	53	47	11
superpathway of unsaturated fatty acids biosynthesis (E. coli)	20	17	4
cis-vaccenate biosynthesis	5	4	1
8-amino-7-oxononanoate biosynthesis IV	5	4	1
superpathway of fatty acid biosynthesis I (E. coli)	16	14	3
superpathway of fatty acid biosynthesis II (plant)	43	37	8
8-amino-7-oxononanoate biosynthesis I	11	9	2
odd iso-branched-chain fatty acid biosynthesis	34	25	6
even iso-branched-chain fatty acid biosynthesis	34	25	6
anteiso-branched-chain fatty acid biosynthesis	34	25	6
(5Z)-dodecenoate biosynthesis I	6	5	1
stearate biosynthesis II (bacteria and plants)	6	5	1
(5Z)-dodecenoate biosynthesis II	6	4	1
streptorubin B biosynthesis	34	20	5
biotin biosynthesis I	15	13	2
mycolate biosynthesis	205	23	4
superpathway of mycolate biosynthesis	239	24	4