Experiment set13IT090 for Pseudomonas fluorescens FW300-N2E2

LB with Cobalt chloride hexahydrate 0.4 mM

Group: stress
Media: LB + Cobalt chloride hexahydrate (0.4 mM)
Culturing: pseudo6_N2E2_ML5b, 48 well microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Adam on 25-Jul-16
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: Plate2 E5,E6

Specific Phenotypes

For 6 genes in this experiment

For stress Cobalt chloride hexahydrate in Pseudomonas fluorescens FW300-N2E2

For stress Cobalt chloride hexahydrate across organisms

SEED Subsystems

Subsystem	#Specific
Arginine and Ornithine Degradation	1
DNA repair, bacterial MutL-MutS system	1
Glycine and Serine Utilization	1
Pyruvate Alanine Serine Interconversions	1
Threonine anaerobic catabolism gene cluster	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Arginine and proline metabolism

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-ornithine degradation I (L-proline biosynthesis)	1	1	1
L-proline biosynthesis II (from arginine)	2	1	1
L-arginine degradation VII (arginase 3 pathway)	2	1	1