Experiment set13IT079 for Pseudomonas fluorescens FW300-N2E2

LB with Nickel (II) chloride hexahydrate 1.5 mM

Group: stress
Media: LB + Nickel (II) chloride hexahydrate (1.5 mM)
Culturing: pseudo6_N2E2_ML5b, 48 well microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Adam on 25-Jul-16
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: Plate1 A5,A6

Specific Phenotypes

For 5 genes in this experiment

For stress Nickel (II) chloride hexahydrate in Pseudomonas fluorescens FW300-N2E2

For stress Nickel (II) chloride hexahydrate across organisms

SEED Subsystems

Subsystem	#Specific
Alginate metabolism	1
Copper homeostasis: copper tolerance	1
Folate Biosynthesis	1
Glutamate dehydrogenases	1
Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis	1
Glycerolipid and Glycerophospholipid Metabolism in Bacteria	1
Mannose Metabolism	1
Phosphate metabolism	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Glycerophospholipid metabolism
• Fructose and mannose metabolism
• Fatty acid biosynthesis
• Glutamate metabolism
• Lysine degradation
• Arginine and proline metabolism
• Histidine metabolism
• Tyrosine metabolism
• Phenylalanine metabolism
• Tryptophan metabolism
• Nucleotide sugars metabolism
• Lipopolysaccharide biosynthesis
• Glycerolipid metabolism
• Ether lipid metabolism
• Glycosphingolipid biosynthesis - ganglio series
• 1- and 2-Methylnaphthalene degradation
• Benzoate degradation via CoA ligation
• Ethylbenzene degradation
• Butanoate metabolism
• One carbon pool by folate
• Methane metabolism
• Folate biosynthesis
• Limonene and pinene degradation
• Diterpenoid biosynthesis
• Carotenoid biosynthesis - General
• Nitrogen metabolism
• Anthocyanin biosynthesis
• Alkaloid biosynthesis I
• Alkaloid biosynthesis II
• Biosynthesis of unsaturated fatty acids
• Biosynthesis of type II polyketide backbone
• Biosynthesis of phenylpropanoids
• Biosynthesis of terpenoids and steroids
• Biosynthesis of alkaloids derived from terpenoid and polyketide

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
L-glutamate degradation I	1	1	1
D-mannose degradation I	2	1	1
D-mannose degradation II	2	1	1
dTMP de novo biosynthesis (mitochondrial)	3	3	1
tetrahydrofolate biosynthesis I	3	3	1
L-alanine degradation II (to D-lactate)	3	3	1
β-1,4-D-mannosyl-N-acetyl-D-glucosamine degradation	3	2	1
oleate biosynthesis III (cyanobacteria)	3	2	1
mannitol biosynthesis	3	1	1
ethene biosynthesis IV (engineered)	3	1	1
CDP-diacylglycerol biosynthesis I	4	4	1
GDP-mannose biosynthesis	4	4	1
CDP-diacylglycerol biosynthesis II	4	4	1
tetrahydromonapterin biosynthesis	4	3	1
mannitol degradation II	4	2	1
phosphatidate biosynthesis (yeast)	5	3	1
1,5-anhydrofructose degradation	5	2	1
phosphatidylglycerol biosynthesis I	6	6	1
phosphatidylglycerol biosynthesis II	6	6	1
superpathway of phospholipid biosynthesis III (E. coli)	12	11	2
palmitoyl ethanolamide biosynthesis	6	2	1
superpathway of stearidonate biosynthesis (cyanobacteria)	6	2	1
diacylglycerol and triacylglycerol biosynthesis	7	3	1
L-glutamate degradation XI (reductive Stickland reaction)	7	3	1
stigma estolide biosynthesis	7	2	1
4-aminobutanoate degradation V	7	2	1
β-(1,4)-mannan degradation	7	2	1
anandamide biosynthesis II	8	2	1
folate transformations III (E. coli)	9	9	1
superpathway of tetrahydrofolate biosynthesis	10	8	1
L-glutamate degradation V (via hydroxyglutarate)	10	6	1
folate transformations II (plants)	11	10	1
colanic acid building blocks biosynthesis	11	9	1
superpathway of tetrahydrofolate biosynthesis and salvage	12	10	1
anandamide biosynthesis I	12	3	1
superpathway of cardiolipin biosynthesis (bacteria)	13	10	1
superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis	14	7	1
superpathway of phospholipid biosynthesis II (plants)	28	10	2
methylaspartate cycle	19	9	1
superpathway of chorismate metabolism	59	43	1