Experiment set13IT072 for Pseudomonas fluorescens FW300-N2E2

Compare to:

LB with Cobalt chloride hexahydrate 0.4 mM

Group: stress
Media: LB + Cobalt chloride hexahydrate (0.4 mM)
Culturing: pseudo6_N2E2_ML5b, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
Growth: about 4.9 generations
By: Adam on 25-Jul-16
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: Plate2 D1

Specific Phenotypes

For 8 genes in this experiment

For stress Cobalt chloride hexahydrate in Pseudomonas fluorescens FW300-N2E2

For stress Cobalt chloride hexahydrate across organisms

SEED Subsystems

Subsystem #Specific
Arginine and Ornithine Degradation 1
Carboxysome 1
DNA repair, bacterial MutL-MutS system 1
Glycine and Serine Utilization 1
Pyruvate Alanine Serine Interconversions 1
Respiratory dehydrogenases 1 1
Threonine anaerobic catabolism gene cluster 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-ornithine degradation I (L-proline biosynthesis) 1 1 1
NADH to cytochrome bo oxidase electron transfer II 2 2 1
NADH to cytochrome bo oxidase electron transfer I 2 2 1
L-proline biosynthesis II (from arginine) 2 1 1
L-arginine degradation VII (arginase 3 pathway) 2 1 1
NADH to nitrate electron transfer 2 1 1
NADH to cytochrome bd oxidase electron transfer I 2 1 1
NADH to cytochrome bd oxidase electron transfer II 2 1 1
nitrate reduction VIIIb (dissimilatory) 2 1 1
NADH to cytochrome aa3 oxidase electron transfer 2 1 1
aerobic respiration III (alternative oxidase pathway) 3 3 1
aerobic respiration II (cytochrome c) (yeast) 4 3 1
aerobic respiration I (cytochrome c) 4 3 1
mitochondrial NADPH production (yeast) 5 4 1
Fe(II) oxidation 6 3 1
NAD(P)/NADPH interconversion 6 3 1
superpathway of NAD/NADP - NADH/NADPH interconversion (yeast) 8 7 1