Experiment set13IT064 for Pseudomonas fluorescens FW300-N2E2

Compare to:

LB with Cobalt chloride hexahydrate 0.6 mM

200 most detrimental genes:

  gene name fitness t score description  
Pf6N2E2_3192 +9.2 21.2 tRNA:Cm32/Um32 methyltransferase compare
Pf6N2E2_3942 +4.7 31.7 FIG001592: Phosphocarrier protein kinase/phosphorylase, nitrogen regulation associated compare
Pf6N2E2_2252 +4.2 12.3 NreA-like protein compare
Pf6N2E2_3941 +3.6 5.4 Adenosine (5')-pentaphospho-(5'')-adenosine pyrophosphohydrolase (EC 3.6.1.-) compare
Pf6N2E2_463 +3.6 16.6 Transcriptional regulatory protein RstA compare
Pf6N2E2_4795 +3.6 6.8 Acyl-phosphate:glycerol-3-phosphate O-acyltransferase PlsY compare
Pf6N2E2_4803 +3.5 5.5 Bis(5'-nucleosyl)-tetraphosphatase, symmetrical (EC 3.6.1.41) compare
Pf6N2E2_5160 +3.4 3.8 C4-type zinc finger protein, DksA/TraR family compare
Pf6N2E2_2936 +3.3 24.0 Cysteine synthase (EC 2.5.1.47) conserved
Pf6N2E2_4068 +3.2 26.9 Phosphate regulon metal ion transporter containing CBS domains compare
Pf6N2E2_5508 +3.1 4.3 Formyltetrahydrofolate deformylase (EC 3.5.1.10) compare
Pf6N2E2_4754 +3.1 23.7 Protein rarD compare
Pf6N2E2_464 +3.0 5.4 Sensory histidine kinase QseC compare
Pf6N2E2_2253 +2.8 2.7 hypothetical protein compare
Pf6N2E2_5274 +2.8 15.9 FolM Alternative dihydrofolate reductase 1 conserved
Pf6N2E2_5015 +2.8 13.5 Magnesium and cobalt efflux protein CorC compare
Pf6N2E2_5272 +2.8 10.9 Dihydroneopterin triphosphate epimerase compare
Pf6N2E2_3663 +2.8 14.6 Glycosyl transferase compare
Pf6N2E2_2278 +2.8 22.1 Transcription-repair coupling factor compare
Pf6N2E2_4601 +2.7 4.5 Hypothetical protein YggS, proline synthase co-transcribed bacterial homolog PROSC compare
Pf6N2E2_4204 +2.6 3.5 rRNA small subunit 7-methylguanosine (m7G) methyltransferase GidB compare
Pf6N2E2_3152 +2.4 13.5 Magnesium and cobalt efflux protein CorC conserved
Pf6N2E2_5084 +2.3 16.5 Glutathione-regulated potassium-efflux system ATP-binding protein compare
Pf6N2E2_4353 +2.3 13.1 Hydrogen peroxide-inducible genes activator compare
Pf6N2E2_4454 +2.3 10.3 Exopolyphosphatase (EC 3.6.1.11) compare
Pf6N2E2_2872 +2.2 16.4 Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3) compare
Pf6N2E2_5420 +2.2 10.8 Cytosol aminopeptidase PepA (EC 3.4.11.1) compare
Pf6N2E2_987 +2.2 2.3 hypothetical protein compare
Pf6N2E2_2013 +2.1 1.6 hypothetical protein compare
Pf6N2E2_2254 +2.0 5.0 Cell filamentation protein fic compare
Pf6N2E2_5989 +2.0 15.9 Transcriptional regulator, GntR family domain / Aspartate aminotransferase (EC 2.6.1.1) compare
Pf6N2E2_5794 +1.9 3.7 Ribosomal large subunit pseudouridine synthase A (EC 4.2.1.70) compare
Pf6N2E2_3918 +1.7 8.3 D-cysteine desulfhydrase (EC 4.4.1.15) conserved
Pf6N2E2_5593 +1.7 6.2 Leucyl-tRNA synthetase compare
Pf6N2E2_2067 +1.7 16.3 cis/trans isomerase conserved
Pf6N2E2_5236 +1.7 3.3 hypothetical protein compare
Pf6N2E2_3114 +1.7 10.8 Transcriptional regulator, TetR family compare
Pf6N2E2_2384 +1.6 3.3 CmpX compare
Pf6N2E2_3493 +1.6 5.0 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100) compare
Pf6N2E2_6077 +1.6 7.2 Cysteine desulfurase (EC 2.8.1.7) compare
Pf6N2E2_4624 +1.6 2.1 Phosphoribosyl-dephospho-CoA transferase (EC 2.7.7.-) compare
Pf6N2E2_5030 +1.6 8.9 Transcriptional regulator, AsnC family compare
Pf6N2E2_4638 +1.5 4.2 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20) compare
Pf6N2E2_3828 +1.5 4.6 tRNA (cytidine(34)-2'-O)-methyltransferase (EC 2.1.1.207) compare
Pf6N2E2_423 +1.5 1.5 Putative phosphatase YfbT compare
Pf6N2E2_3317 +1.5 10.8 Ornithine cyclodeaminase (EC 4.3.1.12) compare
Pf6N2E2_3134 +1.5 2.3 hypothetical protein compare
Pf6N2E2_2845 +1.5 9.5 RNA polymerase associated protein RapA (EC 3.6.1.-) compare
Pf6N2E2_2329 +1.5 9.2 diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s) compare
Pf6N2E2_1089 +1.4 1.9 Delta 1-piperideine-2-carboxylate reductase (EC 1.5.1.21) / Delta 1-pyrroline-2-carboxylate reductase (EC 1.5.1.1) compare
Pf6N2E2_3300 +1.4 2.0 Predicted D-glucarate or D-galactorate regulator, GntR family compare
Pf6N2E2_5132 +1.4 10.1 NADH dehydrogenase (EC 1.6.99.3) conserved
Pf6N2E2_3144 +1.4 9.9 Thiol:disulfide interchange protein DsbC conserved
Pf6N2E2_2465 +1.4 6.0 Cys regulon transcriptional activator CysB compare
Pf6N2E2_144 +1.4 13.4 Uncharacterized protein YtfM precursor compare
Pf6N2E2_145 +1.4 11.6 putative exported protein compare
Pf6N2E2_1169 +1.4 2.9 TnpR protein compare
Pf6N2E2_5242 +1.4 2.2 Malate:quinone oxidoreductase (EC 1.1.5.4) compare
Pf6N2E2_3074 +1.4 2.1 FIG00960973: hypothetical protein compare
Pf6N2E2_5448 +1.3 5.9 hypothetical protein compare
Pf6N2E2_2466 +1.3 6.6 Universal stress protein family compare
Pf6N2E2_5988 +1.3 2.9 hypothetical protein compare
Pf6N2E2_5491 +1.3 9.5 FOG: GGDEF domain conserved
Pf6N2E2_3335 +1.3 10.7 Uncharacterized iron-regulated membrane protein; Iron-uptake factor PiuB compare
Pf6N2E2_178 +1.3 1.2 Phenylalanyl-tRNA synthetase beta chain (EC 6.1.1.20) compare
Pf6N2E2_847 +1.3 4.0 Phosphoribosyl transferase domain protein compare
Pf6N2E2_1203 +1.3 2.9 Isoquinoline 1-oxidoreductase alpha subunit (EC 1.3.99.16) compare
Pf6N2E2_291 +1.3 3.8 ATP-dependent Clp protease adaptor protein ClpS compare
Pf6N2E2_5761 +1.2 2.5 Transcriptional regulator, TetR family compare
Pf6N2E2_988 +1.2 3.4 4-hydroxyphenylpyruvate dioxygenase (EC 1.13.11.27) compare
Pf6N2E2_2217 +1.2 4.9 ATP-dependent Clp protease ATP-binding subunit ClpX compare
Pf6N2E2_5237 +1.2 2.6 Hypoxanthine-guanine phosphoribosyltransferase (EC 2.4.2.8) compare
Pf6N2E2_2587 +1.2 9.2 Serine phosphatase RsbU, regulator of sigma subunit compare
Pf6N2E2_1718 +1.2 2.5 Anthranilate synthase, amidotransferase component (EC 4.1.3.27) compare
Pf6N2E2_5207 +1.2 2.9 hypothetical protein compare
Pf6N2E2_156 +1.2 2.5 Carbon storage regulator compare
Pf6N2E2_3328 +1.2 1.6 transcriptional regulator compare
Pf6N2E2_4640 +1.2 2.0 hypothetical protein compare
Pf6N2E2_2438 +1.2 1.6 hypothetical protein compare
Pf6N2E2_4418 +1.2 0.9 Diaminopimelate epimerase (EC 5.1.1.7) compare
Pf6N2E2_2707 +1.1 3.9 Allophanate hydrolase 2 subunit 1 (EC 3.5.1.54) compare
Pf6N2E2_5504 +1.1 4.3 INTEGRAL MEMBRANE PROTEIN (Rhomboid family) compare
Pf6N2E2_3171 +1.1 1.3 hypothetical protein compare
Pf6N2E2_4073 +1.1 6.6 Phosphate transport system permease protein PstA (TC 3.A.1.7.1) compare
Pf6N2E2_3630 +1.1 4.7 Phosphoserine phosphatase (EC 3.1.3.3) compare
Pf6N2E2_2674 +1.1 1.6 FIG00953856: hypothetical protein compare
Pf6N2E2_2508 +1.1 3.1 hypothetical protein compare
Pf6N2E2_701 +1.1 3.7 Heme d1 biosynthesis protein NirD / Heme d1 biosynthesis protein NirL compare
Pf6N2E2_985 +1.1 7.0 Multidrug resistance transporter, Bcr/CflA family compare
Pf6N2E2_2133 +1.1 4.8 Putative sulfate permease compare
Pf6N2E2_4987 +1.1 1.0 MaoC-like domain protein compare
Pf6N2E2_2312 +1.1 1.3 Cytochrome c oxidase subunit CcoQ (EC 1.9.3.1) compare
Pf6N2E2_295 +1.1 2.8 Leucyl/phenylalanyl-tRNA--protein transferase (EC 2.3.2.6) compare
Pf6N2E2_2469 +1.1 4.4 hypothetical protein compare
Pf6N2E2_819 +1.1 1.8 Response regulator compare
Pf6N2E2_3676 +1.0 7.9 Phosphodiesterase/alkaline phosphatase D-like compare
Pf6N2E2_5860 +1.0 7.7 Branched-chain amino acid transport system carrier protein compare
Pf6N2E2_4121 +1.0 3.4 Ribosomal RNA small subunit methyltransferase B (EC 2.1.1.-) compare
Pf6N2E2_4072 +1.0 6.4 Phosphate transport ATP-binding protein PstB (TC 3.A.1.7.1) compare
Pf6N2E2_2471 +1.0 7.4 Glutamate synthase [NADPH] large chain (EC 1.4.1.13) compare
Pf6N2E2_2886 +1.0 8.3 Phosphogluconate repressor HexR, RpiR family compare
Pf6N2E2_3901 +1.0 1.7 Ribosome-associated heat shock protein implicated in the recycling of the 50S subunit (S4 paralog) compare
Pf6N2E2_3507 +1.0 3.3 FIG014328: hypothetical protein compare
Pf6N2E2_1129 +1.0 2.8 Sensor histidine kinase compare
Pf6N2E2_490 +1.0 2.4 PhaK-like protein compare
Pf6N2E2_2712 +1.0 5.0 Permeases of the major facilitator superfamily compare
Pf6N2E2_2750 +1.0 5.3 Putative permease PerM (= YfgO) compare
Pf6N2E2_3606 +1.0 6.2 23S rRNA (guanosine-2'-O-) -methyltransferase rlmB (EC 2.1.1.-) conserved
Pf6N2E2_4802 +1.0 5.3 Thiosulfate sulfurtransferase GlpE (EC 2.8.1.1) compare
Pf6N2E2_4753 +1.0 4.0 Glycine cleavage system transcriptional antiactivator GcvR compare
Pf6N2E2_2480 +1.0 1.1 FIG00957636: hypothetical protein compare
Pf6N2E2_587 +1.0 4.5 Hydroxyacylglutathione hydrolase (EC 3.1.2.6) compare
Pf6N2E2_3672 +1.0 6.0 Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42) compare
Pf6N2E2_1182 +1.0 2.4 Transcriptional regulator, LysR family compare
Pf6N2E2_3480 +1.0 1.4 Transcriptional regulator, ArsR family compare
Pf6N2E2_3346 +1.0 4.8 Quinolinate phosphoribosyltransferase [decarboxylating] (EC 2.4.2.19) compare
Pf6N2E2_5510 +1.0 2.1 FIG00955324: hypothetical protein compare
Pf6N2E2_3807 +0.9 4.7 Histidine utilization repressor compare
Pf6N2E2_4439 +0.9 5.7 Glutathione-regulated potassium-efflux system ATP-binding protein compare
Pf6N2E2_4194 +0.9 6.6 Two-component response regulator conserved
Pf6N2E2_5500 +0.9 3.0 MoxR-like ATPases compare
Pf6N2E2_862 +0.9 2.9 Fumarylacetoacetate hydrolase family protein compare
Pf6N2E2_3261 +0.9 5.4 hypothetical protein compare
Pf6N2E2_2552 +0.9 5.0 Cytochrome c heme lyase subunit CcmH compare
Pf6N2E2_3151 +0.9 3.1 Glutamate synthase [NADPH] small chain (EC 1.4.1.13) compare
Pf6N2E2_728 +0.9 2.3 Nitrate/nitrite transporter compare
Pf6N2E2_5722 +0.9 3.4 23S rRNA (Uracil-5-) -methyltransferase RumA (EC 2.1.1.-) ## LSU rRNA m(5)U1939 compare
Pf6N2E2_648 +0.9 1.6 General secretion pathway protein K compare
Pf6N2E2_4437 +0.9 3.4 Twin-arginine translocation protein TatB compare
Pf6N2E2_4029 +0.9 4.3 Biofilm PGA synthesis N-glycosyltransferase PgaC (EC 2.4.-.-) compare
Pf6N2E2_4074 +0.9 6.8 Phosphate transport system permease protein PstC (TC 3.A.1.7.1) compare
Pf6N2E2_1140 +0.9 2.0 Polymyxin resistance protein ArnC, glycosyl transferase (EC 2.4.-.-) compare
Pf6N2E2_4766 +0.9 7.4 Acyl-CoA dehydrogenase (EC 1.3.8.7) compare
Pf6N2E2_5329 +0.9 4.0 Alpha/beta hydrolase compare
Pf6N2E2_5583 +0.9 3.9 Bacterioferritin conserved
Pf6N2E2_3158 +0.9 5.6 tRNA (5-methoxyuridine) 34 synthase compare
Pf6N2E2_3077 +0.9 2.6 FIG138056: a glutathione-dependent thiol reductase compare
Pf6N2E2_3641 +0.9 2.6 ADP-ribose pyrophosphatase (EC 3.6.1.13) compare
Pf6N2E2_1303 +0.9 2.0 TRAP-type transport system, small permease component, predicted N-acetylneuraminate transporter compare
Pf6N2E2_2693 +0.9 0.8 3-oxoacyl-[acyl-carrier-protein] synthase, KASIII (EC 2.3.1.180) compare
Pf6N2E2_70 +0.9 2.4 tRNA pseudouridine synthase A (EC 4.2.1.70) compare
Pf6N2E2_414 +0.9 2.1 Chemotaxis protein CheD compare
Pf6N2E2_218 +0.9 2.2 hypothetical protein compare
Pf6N2E2_4762 +0.9 1.9 Biotin synthesis protein BioH compare
Pf6N2E2_1322 +0.9 2.3 MoaE protein compare
Pf6N2E2_5416 +0.9 4.7 Ribosomal RNA large subunit methyltransferase F (EC 2.1.1.51) compare
Pf6N2E2_2468 +0.9 3.8 2-dehydropantoate 2-reductase (EC 1.1.1.169) compare
Pf6N2E2_5595 +0.9 3.7 Electron transport complex protein RnfE compare
Pf6N2E2_1673 +0.8 1.2 Transcriptional regulator, TetR family compare
Pf6N2E2_2467 +0.8 1.8 5'-nucleotidase (EC 3.1.3.5) compare
Pf6N2E2_3190 +0.8 1.2 Iron-sulfur cluster regulator IscR compare
Pf6N2E2_4734 +0.8 4.9 Acyltransferase family protein compare
Pf6N2E2_5775 +0.8 2.5 FOG: TPR repeat, SEL1 subfamily compare
Pf6N2E2_240 +0.8 3.5 L-proline glycine betaine ABC transport system permease protein ProV (TC 3.A.1.12.1) compare
Pf6N2E2_4028 +0.8 4.8 Biofilm PGA synthesis auxiliary protein PgaD conserved
Pf6N2E2_2984 +0.8 1.9 LysR family transcriptional regulator PA3398 compare
Pf6N2E2_3849 +0.8 2.2 FIG00954739: hypothetical protein compare
Pf6N2E2_5250 +0.8 1.4 Restriction endonuclease compare
Pf6N2E2_3457 +0.8 3.4 Uncharacterized protein YfiR precursor compare
Pf6N2E2_600 +0.8 1.8 Diadenosine tetraphosphatase and related serine/threonine protein phosphatases compare
Pf6N2E2_922 +0.8 1.7 FIG00794167: hypothetical protein compare
Pf6N2E2_4555 +0.8 5.3 Lysophospholipase (EC 3.1.1.5) compare
Pf6N2E2_773 +0.8 3.9 Undecaprenyl-diphosphatase (EC 3.6.1.27) compare
Pf6N2E2_3318 +0.8 3.9 putative non-ribosomal peptide synthetase compare
Pf6N2E2_1438 +0.8 2.0 Methyltransferase (EC 2.1.1.-) compare
Pf6N2E2_5505 +0.8 1.5 hypothetical protein compare
Pf6N2E2_544 +0.8 1.9 Short-chain dehydrogenase/reductase SDR compare
Pf6N2E2_2229 +0.8 3.1 Major facilitator family transporter compare
Pf6N2E2_5502 +0.8 3.9 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases compare
Pf6N2E2_3373 +0.8 2.4 Outer membrane lipoprotein SmpA, a component of the essential YaeT outer-membrane protein assembly complex compare
Pf6N2E2_3319 +0.8 3.5 Peptide synthetase compare
Pf6N2E2_3157 +0.8 3.8 tRNA (uridine-5-oxyacetic acid methyl ester) 34 synthase compare
Pf6N2E2_5133 +0.8 1.5 FIG00953980: hypothetical protein compare
Pf6N2E2_5487 +0.8 2.6 Nitrogen regulation protein NR(I) compare
Pf6N2E2_4225 +0.8 2.9 Nicotinamidase (EC 3.5.1.19) compare
Pf6N2E2_2509 +0.8 0.9 UDP-glucose 4-epimerase (EC 5.1.3.2) compare
Pf6N2E2_1313 +0.8 1.7 2-oxo-hepta-3-ene-1,7-dioic acid hydratase (EC 4.2.-.-) compare
Pf6N2E2_4817 +0.7 6.6 Spermidine Putrescine ABC transporter permease component PotB (TC 3.A.1.11.1) compare
Pf6N2E2_5961 +0.7 3.8 Transcriptional regulator, TetR family compare
Pf6N2E2_1504 +0.7 1.7 putative 2-pyrone-4,6-dicarboxylic acid hydrolase compare
Pf6N2E2_5240 +0.7 3.6 FIG00953775: hypothetical protein compare
Pf6N2E2_440 +0.7 1.7 FIG00954920: hypothetical protein compare
Pf6N2E2_4107 +0.7 5.3 Cytochrome oxidase biogenesis protein Sco1/SenC/PrrC, putative copper metallochaperone compare
Pf6N2E2_522 +0.7 1.4 Inositol transport system sugar-binding protein compare
Pf6N2E2_458 +0.7 4.6 Protein rarD compare
Pf6N2E2_3761 +0.7 6.2 Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-) compare
Pf6N2E2_5681 +0.7 4.9 RhtB family transporter compare
Pf6N2E2_5925 +0.7 4.9 Transglutaminase-like enzymes, putative cysteine proteases compare
Pf6N2E2_4193 +0.7 5.7 Signal transduction histidine kinase conserved
Pf6N2E2_704 +0.7 1.2 Cytochrome c551/c552 compare
Pf6N2E2_2871 +0.7 2.3 FIG00954271: hypothetical protein compare
Pf6N2E2_2978 +0.7 4.1 Ribosomal protein S12p Asp88 (E. coli) methylthiotransferase compare
Pf6N2E2_4362 +0.7 1.3 DNA-directed RNA polymerase omega subunit (EC 2.7.7.6) compare
Pf6N2E2_1859 +0.7 1.7 hypothetical protein compare
Pf6N2E2_2325 +0.7 2.4 Recombination protein RecR compare
Pf6N2E2_74 +0.7 1.6 DedD protein compare
Pf6N2E2_4063 +0.7 3.0 Chorismate--pyruvate lyase (EC 4.1.3.40) compare
Pf6N2E2_1178 +0.7 1.7 3-oxoacyl-(acyl-carrier-protein) synthase compare
Pf6N2E2_1917 +0.7 1.4 hypothetical protein compare
Pf6N2E2_5901 +0.7 3.9 hypothetical protein compare


Specific Phenotypes

For 24 genes in this experiment

For stress Cobalt chloride hexahydrate in Pseudomonas fluorescens FW300-N2E2

For stress Cobalt chloride hexahydrate across organisms