Experiment set13IT060 for Pseudomonas fluorescens FW300-N2E2
LB with Nickel (II) chloride hexahydrate 1 mM
Group: stressMedia: LB + Nickel (II) chloride hexahydrate (1 mM)
Culturing: pseudo6_N2E2_ML5b, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
Growth: about 4.6 generations
By: Adam on 25-Jul-16
Media components: 10 g/L Tryptone, 5 g/L Yeast Extract, 5 g/L Sodium Chloride
Growth plate: Plate1 A2
Specific Phenotypes
For 3 genes in this experiment
For stress Nickel (II) chloride hexahydrate in Pseudomonas fluorescens FW300-N2E2
For stress Nickel (II) chloride hexahydrate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Alginate metabolism | 1 |
| Mannose Metabolism | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| D-mannose degradation II | 2 | 1 | 1 |
| D-mannose degradation I | 2 | 1 | 1 |
| β-1,4-D-mannosyl-N-acetyl-D-glucosamine degradation | 3 | 2 | 1 |
| mannitol biosynthesis | 3 | 1 | 1 |
| GDP-mannose biosynthesis | 4 | 4 | 1 |
| mannitol degradation II | 4 | 2 | 1 |
| 1,5-anhydrofructose degradation | 5 | 2 | 1 |
| β-(1,4)-mannan degradation | 7 | 2 | 1 |
| colanic acid building blocks biosynthesis | 11 | 9 | 1 |
| superpathway of GDP-mannose-derived O-antigen building blocks biosynthesis | 14 | 7 | 1 |