Experiment set13IT051 for Klebsiella michiganensis M5al

Compare to:

Fungal_growth_media_mCAFEs_v1 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL

Group: mixed community
Media: Fungal_growth_media_mCAFEs_v1 + Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Culturing: Koxy_ML2a, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Trenton on 7/18/2018
Media components: 20 g/L D-Glucose, 0.2 g/L Magnesium Sulfate Heptahydrate, 0.9 g/L Potassium phosphate dibasic, 0.2 g/L Potassium Chloride, 1 g/L Ammonium Nitrate, 0.002 g/L Iron (II) sulfate heptahydrate, 0.002 g/L Zinc sulfate heptahydrate, 0.002 g/L Manganese (II) chloride tetrahydrate, 0.002 g/L Cobalt chloride hexahydrate
Growth plate: Plate 3 A3

Specific Phenotypes

For 8 genes in this experiment

For mixed community Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL in Klebsiella michiganensis M5al

For mixed community Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL across organisms

SEED Subsystems

Subsystem #Specific
Campylobacter Iron Metabolism 2
Biotin biosynthesis 1
Copper homeostasis: copper tolerance 1
Iron acquisition in Vibrio 1
Phosphate metabolism 1
Pyridoxin (Vitamin B6) Biosynthesis 1
Transport of Iron 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
biotin biosynthesis from 8-amino-7-oxononanoate I 4 4 2
biotin biosynthesis from 8-amino-7-oxononanoate II 4 3 2
biotin biosynthesis from 8-amino-7-oxononanoate III 5 3 2
biotin biosynthesis II 6 4 2
pyridoxal 5'-phosphate biosynthesis I 7 7 1
biotin biosynthesis I 15 15 2
superpathway of pyridoxal 5'-phosphate biosynthesis and salvage 12 12 1