Experiment set13IT049 for Klebsiella michiganensis M5al

Fungal_growth_media_mCAFEs_v1 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL

Group: mixed community
Media: Fungal_growth_media_mCAFEs_v1 + Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Culturing: Koxy_ML2a, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Trenton on 7/18/2018
Media components: 20 g/L D-Glucose, 0.2 g/L Magnesium Sulfate Heptahydrate, 0.9 g/L Potassium phosphate dibasic, 0.2 g/L Potassium Chloride, 1 g/L Ammonium Nitrate, 0.002 g/L Iron (II) sulfate heptahydrate, 0.002 g/L Zinc sulfate heptahydrate, 0.002 g/L Manganese (II) chloride tetrahydrate, 0.002 g/L Cobalt chloride hexahydrate
Growth plate: Plate 3 A1

Specific Phenotypes

For 10 genes in this experiment

For mixed community Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL in Klebsiella michiganensis M5al

For mixed community Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL across organisms

SEED Subsystems

Subsystem	#Specific
Coenzyme A Biosynthesis	2
Biotin biosynthesis	1
Copper homeostasis: copper tolerance	1
Phosphate metabolism	1
Pyridoxin (Vitamin B6) Biosynthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Pantothenate and CoA biosynthesis
• Urea cycle and metabolism of amino groups
• Puromycin biosynthesis
• Arginine and proline metabolism
• beta-Alanine metabolism
• Nucleotide sugars metabolism
• alpha-Linolenic acid metabolism
• Trinitrotoluene degradation
• Vitamin B6 metabolism
• Biotin metabolism
• Porphyrin and chlorophyll metabolism
• Carotenoid biosynthesis - General
• Alkaloid biosynthesis I
• Insect hormone biosynthesis

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
biotin biosynthesis from 8-amino-7-oxononanoate I	4	4	2
phosphopantothenate biosynthesis I	4	4	2
biotin biosynthesis from 8-amino-7-oxononanoate II	4	3	2
biotin biosynthesis from 8-amino-7-oxononanoate III	5	3	2
biotin biosynthesis II	6	4	2
putrescine degradation II	4	4	1
phosphopantothenate biosynthesis III (archaea)	4	2	1
superpathway of coenzyme A biosynthesis I (bacteria)	9	9	2
superpathway of coenzyme A biosynthesis II (plants)	10	5	2
pyridoxal 5'-phosphate biosynthesis I	7	7	1
biotin biosynthesis I	15	15	2
superpathway of ornithine degradation	8	8	1
superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation	11	11	1
superpathway of pyridoxal 5'-phosphate biosynthesis and salvage	12	12	1
superpathway of L-arginine and L-ornithine degradation	13	13	1