Experiment set13IT030 for Desulfovibrio vulgaris Hildenborough JW710

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MoLS4 with TEMED 20mM nitrogen source

Group: nitrogen source
Media: MoLS4_no_ammonium + N,N,N′,N′-Tetramethylethylenediamine (20 mM), pH=7.2
Culturing: DvH_JW710, 24 deep-well microplate, Anaerobic, at 30 (C), shaken=0 rpm
By: Valentine on 6/14/2017
Media components: 30 mM Sodium sulfate, 60 mM Sodium D,L-Lactate, 30 mM Tris hydrochloride, 0.12 mM EDTA, 1 mM Sodium sulfide nonahydrate, 8 mM Magnesium chloride hexahydrate, 0.6 mM Calcium chloride, 2 mM Potassium phosphate dibasic, 60 uM Iron (II) chloride tetrahydrate, Desulfovibrio trace elements (15 uM Manganese (II) chloride tetrahydrate, 7.8 uM Cobalt chloride hexahydrate, 9 uM Zinc chloride, 1.26 uM Sodium molybdate, 1.92 uM Boric Acid, 2.28 uM Nickel (II) sulfate hexahydrate, 0.06 uM copper (II) chloride dihydrate, 0.21 uM Sodium selenite pentahydrate, 0.144 uM Sodium tungstate dihydrate), Thauer's vitamin mix (0.01 mg/L Pyridoxine HCl, 0.005 mg/L 4-Aminobenzoic acid, 0.005 mg/L Lipoic acid, 0.005 mg/L Nicotinic Acid, 0.005 mg/L Riboflavin, 0.005 mg/L Thiamine HCl, 0.005 mg/L calcium pantothenate, 0.002 mg/L biotin, 0.002 mg/L Folic Acid, 0.0001 mg/L Cyanocobalamin, 0.2 mg/L Choline chloride)

Specific Phenotypes

For 10 genes in this experiment

For nitrogen source N,N,N′,N′-Tetramethylethylenediamine in Desulfovibrio vulgaris Hildenborough JW710

For nitrogen source N,N,N′,N′-Tetramethylethylenediamine across organisms

SEED Subsystems

Subsystem #Specific
DNA repair, UvrABC system 2
Bacterial Chemotaxis 1
DNA-replication 1
Flagellar motility 1
Glycine and Serine Utilization 1
Glycine cleavage system 1
Photorespiration (oxidative C2 cycle) 1
Transcription factors bacterial 1
Two-component regulatory systems in Campylobacter 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
glycine biosynthesis II 3 3 1
glycine cleavage 3 3 1