Experiment set13IT027 for Pseudomonas fluorescens SBW25-INTG

Glucose (C) and Sodium nitrate (N); with MOPS

Group: no stress control
Media: MME_noNitrogen_noCarbon + D-Glucose (10 mM) + Sodium nitrate (5 mM), pH=7
Culturing: PseudoSBW25_INTG_ML3, 96 deep-well microplate; 1.2 mL volume, Aerobic, at 30 (C), shaken=1200 rpm
By: Joshua Elmore on September 1, 2021
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)

Specific Phenotypes

For 8 genes in this experiment

For no stress control D-Glucose in Pseudomonas fluorescens SBW25-INTG

For no stress control D-Glucose across organisms

SEED Subsystems

Subsystem	#Specific
Nitrate and nitrite ammonification	5
Ammonia assimilation	1
Coenzyme B12 biosynthesis	1
Dissimilatory nitrite reductase	1
Experimental tye	1
Heme and Siroheme Biosynthesis	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Nitrogen metabolism
• Porphyrin and chlorophyll metabolism
• mTOR signaling pathway

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
siroheme biosynthesis	4	4	3
alkylnitronates degradation	2	2	1
nitrate reduction V (assimilatory)	2	1	1
factor 430 biosynthesis	7	3	3
cob(II)yrinate a,c-diamide biosynthesis I (early cobalt insertion)	15	12	3
cob(II)yrinate a,c-diamide biosynthesis II (late cobalt incorporation)	13	13	2
adenosylcobalamin biosynthesis I (anaerobic)	36	27	3
adenosylcobalamin biosynthesis II (aerobic)	33	30	2