Experiment set13IT015 for Klebsiella michiganensis M5al
Fungal_growth_media_mCAFEs_v1 with Vancomycin Hydrochloride Hydrate 0.01 mg/mL
Group: stressMedia: Fungal_growth_media_mCAFEs_v1 + Vancomycin Hydrochloride Hydrate (0.01 mg/mL)
Culturing: Koxy_ML2a, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Trenton on 7/18/2018
Media components: 20 g/L D-Glucose, 0.2 g/L Magnesium Sulfate Heptahydrate, 0.9 g/L Potassium phosphate dibasic, 0.2 g/L Potassium Chloride, 1 g/L Ammonium Nitrate, 0.002 g/L Iron (II) sulfate heptahydrate, 0.002 g/L Zinc sulfate heptahydrate, 0.002 g/L Manganese (II) chloride tetrahydrate, 0.002 g/L Cobalt chloride hexahydrate
Growth plate: Plate 1 C3
Specific Phenotypes
For 4 genes in this experiment
For stress Vancomycin Hydrochloride Hydrate in Klebsiella michiganensis M5al
For stress Vancomycin Hydrochloride Hydrate across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Coenzyme A Biosynthesis | 1 |
| Copper homeostasis: copper tolerance | 1 |
| Iron acquisition in Vibrio | 1 |
| Phosphate metabolism | 1 |
| Transport of Iron | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Urea cycle and metabolism of amino groups
- Arginine and proline metabolism
- beta-Alanine metabolism
- Pantothenate and CoA biosynthesis
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| phosphopantothenate biosynthesis I | 4 | 4 | 1 |
| putrescine degradation II | 4 | 4 | 1 |
| superpathway of ornithine degradation | 8 | 8 | 1 |
| superpathway of coenzyme A biosynthesis I (bacteria) | 9 | 9 | 1 |
| superpathway of coenzyme A biosynthesis II (plants) | 10 | 5 | 1 |
| superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation | 11 | 11 | 1 |
| superpathway of L-arginine and L-ornithine degradation | 13 | 13 | 1 |