Experiment set13IT009 for Klebsiella michiganensis M5al

Compare to:

Fungal_growth_media_mCAFEs_v1 with Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted 0.8 x and biotin 0.32 ug/ml

Group: supernatant
Media: Fungal_growth_media_mCAFEs_v1 + supernatant from Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted (0.8 x) + biotin (0.32 ug/ml)
Culturing: Koxy_ML2a, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Trenton on 7/18/2018
Media components: 20 g/L D-Glucose, 0.2 g/L Magnesium Sulfate Heptahydrate, 0.9 g/L Potassium phosphate dibasic, 0.2 g/L Potassium Chloride, 1 g/L Ammonium Nitrate, 0.002 g/L Iron (II) sulfate heptahydrate, 0.002 g/L Zinc sulfate heptahydrate, 0.002 g/L Manganese (II) chloride tetrahydrate, 0.002 g/L Cobalt chloride hexahydrate
Growth plate: Plate 1 B3

Specific Phenotypes

For 1 genes in this experiment

For supernatant Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted in Klebsiella michiganensis M5al

For supernatant Supernatant; Trichoderma harzianum T22 grown in Fungal_growth_media_mCAFEs_v1, filtered and pH-adjusted across organisms

SEED Subsystems

Subsystem #Specific
Chorismate: Intermediate for synthesis of PAPA antibiotics, PABA, anthranilate, 3-hydroxyanthranilate and more. 1
Folate Biosynthesis 1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway #Steps #Present #Specific
L-glutamine degradation I 1 1 1
L-glutamate biosynthesis I 2 2 1
4-aminobenzoate biosynthesis I 2 2 1
ammonia assimilation cycle III 3 3 1
glutaminyl-tRNAgln biosynthesis via transamidation 4 3 1
L-asparagine biosynthesis III (tRNA-dependent) 4 3 1
L-glutamate and L-glutamine biosynthesis 7 6 1
L-citrulline biosynthesis 8 6 1
chloramphenicol biosynthesis 9 1 1
superpathway of tetrahydrofolate biosynthesis 10 9 1
superpathway of candicidin biosynthesis 11 3 1
superpathway of tetrahydrofolate biosynthesis and salvage 12 11 1
superpathway of L-citrulline metabolism 12 8 1
superpathway of chorismate metabolism 59 54 1