Experiment set13IT008 for Ralstonia sp. UNC404CL21Col
Dictyostellum sp.mixed culture-2
Group: micoeukaryotesMedia: LB (0.1x) + Pelleted after growth overnight in LB+Kan50 + CaCl2, MgSO4 (10mM ), pH=7
Culturing: CL21_ML4, 48-well_plate, Aerobic, at 30 (C), shaken=700 rpm
By: VM on 6/23/21
Media components: 1 g/L Tryptone, 0.5 g/L Yeast Extract, 0.5 g/L Sodium Chloride (final concentrations)
Specific Phenotypes
For 12 genes in this experiment
For micoeukaryotes Pelleted after growth overnight in LB+Kan50 in Ralstonia sp. UNC404CL21Col
For micoeukaryotes Pelleted after growth overnight in LB+Kan50 across organisms
SEED Subsystems
| Subsystem | #Specific |
|---|---|
| Choline and Betaine Uptake and Betaine Biosynthesis | 1 |
| Phosphorylcholine incorporation in LPS | 1 |
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| choline degradation I | 2 | 2 | 1 |
| glycine betaine biosynthesis I (Gram-negative bacteria) | 2 | 2 | 1 |
| glycine betaine biosynthesis II (Gram-positive bacteria) | 2 | 2 | 1 |
| choline-O-sulfate degradation | 3 | 3 | 1 |