Experiment set13IT006 for Pseudomonas fluorescens SBW25-INTG
Glucose (C) and Ammonium chloride (N); with MOPS; with Sodium chloride
Group: stressMedia: MME_noNitrogen_noCarbon + D-Glucose (10 mM) + Ammonium chloride (10 mM) + Sodium Chloride (400 mM), pH=7
Culturing: PseudoSBW25_INTG_ML3, 96 deep-well microplate; 1.2 mL volume, Aerobic, at 30 (C), shaken=1200 rpm
By: Joshua Elmore on September 1, 2021
Media components: 9.1 mM Potassium phosphate dibasic trihydrate, 20 mM 3-(N-morpholino)propanesulfonic acid, 4.3 mM Sodium Chloride, 0.41 mM Magnesium Sulfate Heptahydrate, 0.07 mM Calcium chloride dihydrate, MME Trace Minerals (0.5 mg/L EDTA tetrasodium tetrahydrate salt, 2 mg/L Ferric chloride, 0.05 mg/L Boric Acid, 0.05 mg/L Zinc chloride, 0.03 mg/L copper (II) chloride dihydrate, 0.05 mg/L Manganese (II) chloride tetrahydrate, 0.05 mg/L Diammonium molybdate, 0.05 mg/L Cobalt chloride hexahydrate, 0.05 mg/L Nickel (II) chloride hexahydrate)
Specific Phenotypes
For 11 genes in this experiment
For stress D-Glucose in Pseudomonas fluorescens SBW25-INTG
For stress D-Glucose across organisms
SEED Subsystems
Metabolic Maps
Color code by fitness: see overview map or list of maps.
Maps containing gene(s) with specific phenotypes:
- Pentose phosphate pathway
- Tryptophan metabolism
- Glutathione metabolism
- One carbon pool by folate
- Methane metabolism
- Folate biosynthesis
- Biosynthesis of phenylpropanoids
- Biosynthesis of alkaloids derived from histidine and purine
- Biosynthesis of alkaloids derived from terpenoid and polyketide
- Biosynthesis of plant hormones
MetaCyc Pathways
Pathways that contain genes with specific phenotypes:
| Pathway | #Steps | #Present | #Specific |
|---|---|---|---|
| dTMP de novo biosynthesis (mitochondrial) | 3 | 3 | 1 |
| tetrahydrofolate biosynthesis I | 3 | 3 | 1 |
| pentose phosphate pathway (oxidative branch) I | 3 | 3 | 1 |
| pentose phosphate pathway | 8 | 8 | 1 |
| folate transformations III (E. coli) | 9 | 9 | 1 |
| superpathway of tetrahydrofolate biosynthesis | 10 | 8 | 1 |
| folate transformations II (plants) | 11 | 10 | 1 |
| superpathway of tetrahydrofolate biosynthesis and salvage | 12 | 10 | 1 |
| superpathway of glucose and xylose degradation | 17 | 16 | 1 |
| superpathway of chorismate metabolism | 59 | 42 | 1 |