Experiment set13IT003 for Klebsiella michiganensis M5al

Compare to:

RCH2_defined_noCarbon with Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days 6 x

Group: supernatant
Media: RCH2_defined_noCarbon (0.5x) + supernatant from Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days (6 x)
Culturing: Koxy_ML2a, 24-well transparent microplate; Multitron, Aerobic, at 30 (C), shaken=700 rpm
By: Trenton on 7/18/2018
Media components: 0.125 g/L Ammonium chloride, 0.05 g/L Potassium Chloride, 0.3 g/L Sodium phosphate monobasic monohydrate, 15 mM PIPES sesquisodium salt, Wolfe's mineral mix (0.015 g/L Magnesium Sulfate Heptahydrate, 0.0075 g/L Nitrilotriacetic acid, 0.005 g/L Sodium Chloride, 0.0025 g/L Manganese (II) sulfate monohydrate, 0.0005 g/L Cobalt chloride hexahydrate, 0.0005 g/L Zinc sulfate heptahydrate, 0.0005 g/L Calcium chloride dihydrate, 0.0005 g/L Iron (II) sulfate heptahydrate, 0.000125 g/L Nickel (II) chloride hexahydrate, 0.0001 g/L Aluminum potassium sulfate dodecahydrate, 5e-05 g/L Copper (II) sulfate pentahydrate, 5e-05 g/L Boric Acid, 5e-05 g/L Sodium Molybdate Dihydrate, 0.0015 mg/L Sodium selenite pentahydrate), Wolfe's vitamin mix (0.05 mg/L Pyridoxine HCl, 0.025 mg/L 4-Aminobenzoic acid, 0.025 mg/L Lipoic acid, 0.025 mg/L Nicotinic Acid, 0.025 mg/L Riboflavin, 0.025 mg/L Thiamine HCl, 0.025 mg/L calcium pantothenate, 0.01 mg/L biotin, 0.01 mg/L Folic Acid, 0.0005 mg/L Cyanocobalamin) (final concentrations)
Growth plate: Plate 1 A3

Specific Phenotypes

None in this experiment

For Klebsiella michiganensis M5al in supernatant experiments

For supernatant Supernatant; Trichoderma harzianum M10 grown as spores in 0.5x MS_media_noCarbon with Brachypodium distachyon roots for 27 days across organisms