Experiment set12S329 for Pantoea sp. MT58

TNT nitrogen source

Group: nitrogen source
Media: M9 minimal media_noNitrogen + Trinitrotoluene (0.44 mM)
Culturing: MT058_ML2
By: Alex Rivier on 01/09/2025
Media components: 4 g/L D-Glucose, 2 mM Magnesium sulfate, 0.1 mM Calcium chloride, 12.8 g/L Sodium phosphate dibasic heptahydrate, 3 g/L Potassium phosphate monobasic, 0.5 g/L Sodium Chloride

Specific Phenotypes

For 23 genes in this experiment

For nitrogen source Trinitrotoluene in Pantoea sp. MT58

For nitrogen source Trinitrotoluene across organisms

SEED Subsystems

Subsystem	#Specific
Multidrug Resistance Efflux Pumps	2
Bacterial hemoglobins	1
CMP-N-acetylneuraminate Biosynthesis	1
DNA-binding regulatory proteins, strays	1
Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)	1
Protein degradation	1
Proteolysis in bacteria, ATP-dependent	1
Sialic Acid Metabolism	1
Transcription factors bacterial	1

Metabolic Maps

Color code by fitness: see overview map or list of maps.

Maps containing gene(s) with specific phenotypes:

• Nucleotide sugars metabolism
• Aminosugars metabolism
• Fructose and mannose metabolism
• Ascorbate and aldarate metabolism
• C21-Steroid hormone metabolism
• Glycine, serine and threonine metabolism
• Bisphenol A degradation
• Polyketide sugar unit biosynthesis
• Linoleic acid metabolism
• Tetrachloroethene degradation
• Benzoate degradation via CoA ligation
• Butanoate metabolism
• Retinol metabolism
• Alkaloid biosynthesis I
• Insect hormone biosynthesis
• Biosynthesis of unsaturated fatty acids
• Biosynthesis of type II polyketide products
• Biosynthesis of alkaloids derived from shikimate pathway
• mTOR signaling pathway

MetaCyc Pathways

Pathways that contain genes with specific phenotypes:

Pathway	#Steps	#Present	#Specific
N-end rule pathway I (prokaryotic)	2	2	2
UDP-N-acetyl-α-D-mannosaminouronate biosynthesis	1	1	1
UDP-N-acetyl-α-D-mannosamine biosynthesis	1	1	1
di-trans,poly-cis-undecaprenyl phosphate biosynthesis	2	2	1
CMP-N-acetylneuraminate biosynthesis II (bacteria)	3	1	1
microcin B17 biosynthesis	3	1	1
polyisoprenoid biosynthesis (E. coli)	5	5	1
Salmonella enterica serotype O:3,10 O antigen biosynthesis	5	1	1
CMP-N-acetylneuraminate biosynthesis I (eukaryotes)	5	1	1
Salmonella enterica serotype O:9,46 O antigen biosynthesis	6	1	1
Salmonella enterica serotype O:9 O antigen biosynthesis	6	1	1
Porphyromonas gingivalis O-LPS antigen biosynthesis	6	1	1
Salmonella enterica serotype O:4 O antigen biosynthesis (group B1)	6	1	1
Salmonella enterica serotype O:2 O antigen biosynthesis	6	1	1
Salmonella enterica serotype O:8 O antigen biosynthesis	8	1	1
Salmonella enterica serotype O:9,46,27 O antigen biosynthesis	9	1	1
superpathway of enterobacterial common antigen biosynthesis	10	8	1
superpathway of UDP-N-acetylglucosamine-derived O-antigen building blocks biosynthesis	24	7	2
succinoglycan biosynthesis	14	1	1
superpathway of CMP-sialic acids biosynthesis	15	2	1